We narrowed to 7,293 results for: ef1a

1. lentiCRISPRv2_ZsG gRNA_4

   Plasmid

   #192688

   Purpose

   Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

   Depositor

   Jeffrey Miner

   Insert

   Cas9, ZsGreen targeting sgRNA #4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a, U6

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPRv2_ZsG gRNA_1

   Plasmid

   #192685

   Purpose

   Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

   Depositor

   Jeffrey Miner

   Insert

   Cas9, ZsGreen targeting sgRNA #1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a, U6

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCRISPRv2_ZsG gRNA_2

   Plasmid

   #192686

   Purpose

   Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

   Depositor

   Jeffrey Miner

   Insert

   Cas9, ZsGreen targeting sgRNA #2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a, U6

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. lentiCRISPRv2_ZsG gRNA_3

   Plasmid

   #192687

   Purpose

   Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

   Depositor

   Jeffrey Miner

   Insert

   Cas9, ZsGreen targeting sgRNA #3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a, U6

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiMPH v2

   Plasmid

   #89308

   Purpose

   lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.

   Depositor

   Feng Zhang

   Article

   Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

   Insert

   MS2-P65-HSF1_2A_Hygro (RELA Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   N55K in MS2

   Promoter

   EF1a

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. phDLX2-N174

   Plasmid

   #60860

   Purpose

   2nd generation lentiviral transfer plasmid. Expresses DLX2 under the EF1a promoter

   Depositor

   Andrew Yoo

   Article

   Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

   Insert

   DLX2 (DLX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. Human USP7-3X FLAG

   Plasmid

   #225334

   Purpose

   Lentiviral expression of human USP7 with 3x FLAG tag in mammalian cells

   Depositor

   Huda Zoghbi

   Article

   Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

   Insert

   USP7 (USP7 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Human USP7_H456A-3X FLAG

   Plasmid

   #225335

   Purpose

   Lentiviral expression of human mutant USP7 with 3x FLAG tag in mammalian cells

   Depositor

   Huda Zoghbi

   Article

   Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

   Insert

   USP7 (USP7 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   H456A

   Promoter

   EF1a

   Available since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AYBEv3

   Plasmid

   #193967

   Purpose

   An engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   ecTadA8e-nSpCas9(D10A)-MPG(v3)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G163R, N169S, S198A, K202A, G203A, S206A, K210A

   Promoter

   hU6, EF1a, CBh

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ10704

    Plasmid

    #183957

    Purpose

    U6-SKSKSO(crRNA array)-EF1a-PuroR-T2A-BFP

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Insert

    SKSKSO array
    

    Use

    Lentiviral

    Tags

    EF1a-Puro-T2A-TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLL7 lenti-dcas9-3xflag-blast

    Plasmid

    #112133

    Purpose

    lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    dCas9(D10A, N863A)-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A mutants in Cas9

    Promoter

    EF1a

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-FLEX-EGFPL10a

    Plasmid

    #98747

    Purpose

    Cre-dependently expresses the fusion protein EGFPL10a

    Depositor

    Alexander Nectow, Eric Schmidt, Nathaniel Heintz

    Article

    Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi

    Has Service

    AAV Retrograde and AAV5

    Insert

    EGFPL10a
    

    Use

    AAV and Cre/Lox

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCKC775

    Plasmid

    #226626

    Purpose

    piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT Human, S. pyogenes)
    EF1a promoter, sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    7xTCF-LEF and EF1a

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCKC764

    Plasmid

    #226625

    Purpose

    piggyBAC pCAG-hCas9-T2A-Unbiased3

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT Human, Chicken, S. pyogenes)
    EF1a promoter, blasticidin resistance
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and EF1a

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCKC776

    Plasmid

    #226627

    Purpose

    piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT Human, S. pyogenes)
    EF1a promoter, mCherry
    

    Use

    Synthetic Biology

    Tags

    oxygen dependent degron (ODD)

    Expression

    Mammalian

    Mutation

    Promoter

    4xHRE_YB TATA and EF1a

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. MCP-MSN

    Plasmid

    #210704

    Purpose

    This plasmid express EF1a promoter driven MCP-MSN transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    MCP fused to transactivation domain from Human transcription factor MRTF-A, STAT-1 and NRF2
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    EF1a

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. gRNA-10Xcapture-PuroR

    Plasmid

    #211496

    Purpose

    Plasmid for the expression of SAM-compatible gRNA for CRISPRa with capturer sequence for 10X Genomics sequencing

    Depositor

    Lesley Forrester, Antonella Fidanza

    Article

    Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

    Inserts

    U6-gRNA
    Ef1a-PuroR
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and U6

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMK1254

    Plasmid

    #133057

    Purpose

    Lentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mRuby2 fusion protein.

    Depositor

    Martin Kampmann

    Article

    Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

    Insert

    Tau.K18(LM)
    

    Use

    Lentiviral

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MCP-eN3x9

    Plasmid

    #210706

    Purpose

    This plasmid express EF1a promoter driven MCP-eN3x9 transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    MCP fused to transactivation domain comprising eNRF2 and 3x 9aa Transactivation domain
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    EF1a

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMK1253

    Plasmid

    #133058

    Purpose

    Lentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-Clover2 fusion protein.

    Depositor

    Martin Kampmann

    Article

    Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

    Insert

    Tau.K18(LM)
    

    Use

    Lentiviral

    Tags

    mClover2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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