We narrowed to 7,174 results for: ef1a

1. pSBbi-Puro_LPAR_AKAR-WT

   Plasmid

   #125206

   Purpose

   SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporter

   Depositor

   Eric Davis

   Insert

   LPAR_AKAR-WT
   

   Use

   Transposon

   Tags

   EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV V5-Senp2

   Plasmid

   #175118

   Purpose

   Lentiviral expression of V5-tagged mouse Senp2

   Depositor

   Larry Gerace

   Insert

   Senp2 (Senp2 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-puro-N-3HA-SREBP1-C-Flag

   Plasmid

   #134286

   Purpose

   Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP1

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   SREBP1 (Srebf1 Mouse)
   

   Use

   Lentiviral

   Tags

   3x HA and Flag

   Expression

   Mammalian

   Mutation

   mouse SREBP1 isoform a precursor

   Promoter

   EF1a

   Available since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. gGBEv6.3-SpCas9

   Plasmid

   #202629

   Purpose

   vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.

   Insert

   nSpCas9(D10A)-MPGv6.3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   MPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202…

   Promoter

   hU6, EF1a, CBh

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. gCBEv2

   Plasmid

   #220617

   Purpose

   vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv2) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

   Insert

   hUNG2-nSpCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)

   Promoter

   hU6, EF1a

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. gTBEv3

   Plasmid

   #220619

   Purpose

   vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv3) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

   Insert

   hUNG2-nSpCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

   Promoter

   hU6, EF1a

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. gGBEv6.3-SpG

   Plasmid

   #202630

   Purpose

   vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpG nickase driven by EF1a promoter, guide RNA compatible with SpG driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.

   Insert

   nSpG(D10A)-MPGv6.3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SpG = D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…

   Promoter

   hU6, EF1a, CBh

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-nEF Con/Foff hChR2(H134R)-EYFP

   Plasmid

   #55647

   Purpose

   Cre-on/Flp-off ChR2-EYFP under the short Ef1a promoter

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   ChR2(H134R)-EYFP
   

   Use

   AAV; Cre on/flp off chr2-eyfp

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Short Ef1a

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiU6-LacZ-GFP-Puro (BB)

   Plasmid

   #170459

   Purpose

   For cloning gRNA block

   Depositor

   Yonglun Luo

   Article

   Xiang et al Nat Commun. 2021 May 28;12(1):3238. doi:

   Insert

   hU6-BB-LacZ and EF1A-EGFP-2A-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6 for gRNA and EF1A for EGFP-2A-Puro

   Available since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS191

    Plasmid

    #197140

    Purpose

    scCloneSelect C-to-T reporter encoding BC-C1 barcode (EF1a promoter version)

    Depositor

    Nozomu Yachie

    Article

    Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    EGFP(M1V)

    Promoter

    EF1a

    Available since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-Hyg-Snrnp40-skywarp-CRISPR-resistant

    Plasmid

    #134252

    Purpose

    Lentivector encoding CRISPR-resistant skywarp form of Snrnp40 (missing exon 5)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

    Insert

    Snrp40 (Snrnp40 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    deleted amino acids 179-219, and mutated coding s…

    Promoter

    EF1a

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. dCas9-miniTurbo

    Plasmid

    #219822

    Purpose

    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)

    Depositor

    Philipp Maass

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    miniTurbo

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    EF1a

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-US-ECasE

    Plasmid

    #83961

    Purpose

    Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.

    Depositor

    Eleanor Chen

    Insert

    ERT2-spCas9-ERT2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    please see depositor comments below

    Promoter

    U6 (gRNA), EF1a (ERT2-Cas9-ERT2)

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-Blast_hCOL4A5

    Plasmid

    #198100

    Purpose

    lentiviral expression of hCOL4A5 in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    COL4A5 (COL4A5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV-AausFP1-Z1

    Plasmid

    #135048

    Purpose

    AausFP1 - bright green fluorescent protein expression from A. australis under CMV promoter in minimal backbone plasmid.

    Depositor

    Jordan Green

    Insert

    AausFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-Blast_hCOL4A5-HA

    Plasmid

    #198103

    Purpose

    lentiviral expression of hCOL4A5 in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    COL4A5 (COL4A5 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cofilin1_pLVX-EF1-Cry2-mCherry

    Plasmid

    #64370

    Purpose

    lentiviral expression of Cofilin1 and Cry2-mCherry

    Depositor

    Matthieu Piel

    Insert

    Cofilin1 (CFL1 Human)
    

    Use

    Lentiviral

    Tags

    Cry2-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

18. TK4-NLS-EGFP

    Plasmid

    #239046

    Purpose

    PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation

    Depositor

    Marius Wernig

    Inserts

    EGFP
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2 and c-myc-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and EF1a

    Available since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-Blast_hCOL4A5-HA_C1567R

    Plasmid

    #198105

    Purpose

    lentiviral expression of hCOL4A5_C1567R in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    COL4A5 (COL4A5 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    C1567R

    Promoter

    EF1a

    Available since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-Blast_hCOL4A5-HA_G869R

    Plasmid

    #198104

    Purpose

    lentiviral expression of hCOL4A5_G869R in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    COL4A5 (COL4A5 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    G869R

    Promoter

    EF1a

    Available since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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