We narrowed to 16,702 results for: nans

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pMSCVpuro-hAIM2-DsRed monomer

Plasmid

#51609

Purpose

Express DsRed monomer-tagged hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Use

Retroviral

Tags

DsRed monomer

Expression

Mammalian

Promoter

MESV

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pMSC234

Plasmid

#216357

Purpose

xhNup93-Nb3t (+2Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup93-Nb3t (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-NLuc-MBP-6xHis

Plasmid

#135931

Purpose

Expresses NanoLuciferase in bacterial cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

NanoLuciferase

Tags

MBP-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pRSETb-rsGreen0.7

Plasmid

#78176

Purpose

Expresses rsGreen0.7 in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen0.7

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

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pMSC226

Plasmid

#216375

Purpose

xhNup358-Nb2t (+3Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup358-Nb2t (+3Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUB-MSP1D1

Plasmid

#161609

Purpose

Bacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

Insert

UD-MSP1D1

Tags

7-His tag

Expression

Bacterial

Mutation

mutant of MSP1D1 ( Q138W, L181W, Y236W)

Promoter

T7

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET24a-AlkB deltaN11

Plasmid

#73622

Purpose

Expresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tag

Depositor

Catherine Drennan

Article

Frick et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

AlkB (alkB E. coli)

Tags

His

Expression

Bacterial

Mutation

deletion of first 11 AA

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
pTP728

Plasmid

#216374

Purpose

xNup358-Nb1t (+3Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xNup358-Nb1t (+3Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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(SUMO)5

Plasmid

#126949

Purpose

Bacterial expression plasmid for (SUMO)5 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

5 repeats of human SUMO3 separated by (GGS)4 (SUMO3 Human)

Expression

Bacterial

Promoter

unknown

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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delCMV-mCherry

Plasmid

#214143

Purpose

Control sensor

Depositor

Leif Dehmelt

Article

Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

Insert

mCherry

Expression

Mammalian

Promoter

delCMV

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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HA-DN-14-3-3 zeta (R56A/R60A)

Plasmid

#116889

Purpose

Expresses HA-tagged human dominant-negative 14-3-3 zeta (R56A/R60A) in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Article

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

DN-14-3-3 zeta (R56A/R60A) (YWHAZ Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-P2A-APEX2-csChBP

Plasmid

#108877

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against mCherry (includes green cytoplasmic reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

EGFP-P2A-APEX2-csChBP

Expression

Mammalian

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-P2A-APEX2-ChBP

Plasmid

#108879

Purpose

Expression of APEX2 fused to a nanobody against mCherry (includes green cytoplasmic reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

EGFP-P2A-APEX2-ChBP

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pre_II_CS3_7653_v2

Plasmid

#126855

Purpose

7653 b scaffold from compatibility group 3, made by method II, containing 1 Zn-dependent self-cleaving DNAzyme cassette for linearisation into 7560 b scaffold

Depositor

Hendrik Dietz

Article

Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

Insert

II_CS3_7653_v2

Use

Synthetic Biology

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pFlag-CMV2-mAIM2

Plasmid

#51540

Purpose

Express Flag-tagged mAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pSN818

Plasmid

#190690

Purpose

Mammalian expression plasmid of anti-kinesin (Mouse). Expresses H2 IgG (heavy chain and light chain).

Depositor

Shinsuke Niwa

Article

Niwa et al Cytoskeleton (Hoboken). 2023 Apr 10. doi

Insert

anti-kinesin (Mus musculus) recombinant mouse monoclonal antibody; IgG heavy and light chains

Expression

Mammalian

Promoter

Dual CMV

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoLAMA-G97

Plasmid

#130705

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-G97

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pFLIPglu-2uDelta13

Plasmid

#12990

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16F D152A Delta13

Use

Plant nanosensor

Tags

ECFP and EYFP

Expression

Plant

Mutation

D152A mutation in binding protein

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-T7-hAIM2-EGFP

Plasmid

#51608

Purpose

Express T7 and GFP-tagged hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Use

Retroviral

Tags

EGFP and T7

Expression

Mammalian

Promoter

MESV

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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RFP-(SUMO FAAA)3

Plasmid

#130703

Purpose

Mammalian expression plasmid for negative control of mCherry-(SUMO)3.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

3 copies of human SUMO1 separated by (GGS)4

Expression

Mammalian

Mutation

FKVK sequence in each SUMO1 module changed to FAAA

Promoter

CMV

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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