Addgene

We narrowed to 4,764 results for: IRES

Showing: 2001 - 2020 of 4764 results

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pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S/C288S

Plasmid

#62460

Purpose

expresses C151S and C288S double mutant human Keap1 protein

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Use

Tags

His-Halo-Tev

Expression

Mammalian

Mutation

Changed Cysteines 151 and 288 of Keap1 to respect…

Promoter

CMV

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE

Plasmid

#89587

Purpose

For nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

nuclear localization signal (nls) turboRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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rAAV-syn::FLEX-rev::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

Plasmid

#32475

Purpose

Cre-dependent expression of PSAM-5HT3 HC (Q79G,Q139G) neuronal activator, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAMQ79G,Q139G-5HT3HC

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

IRES-EGFP

Expression

Mutation

Promoter

Synapsin

Available since

Dec. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pLL3.7 K122 -ires-GFP-Osteocalcin-promoter-H2B mCherry reporter

Plasmid

#68714

Depositor

Yutaka Hata

Insert

H2B mCherry and GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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Chx10 (164bp crit reg+SV40 bas prom)-GFP-IRES-AP

Plasmid

#18808

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

Chx10 (164bp crit reg+SV40 bas prom)-GFP-IRES-AP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only
NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro

Plasmid

#89994

Purpose

Donor template for generation of NGN3-ntdTomato reporter cell lines

Depositor

Timo Otonkoski

Insert

NGN3-T2A-NLS-TdTomato-ires-puro-BGHpA-PGK-puro (NEUROG3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

none

Available since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-mCherry

Plasmid

#122959

Purpose

Lentiviral expression of NOTCH2NL delta C terminus under CAG promoter with mCherry expression

Depositor

Pierre Vanderhaeghen

Article

Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

Insert

NOTCH2NL-delta C terminus (NOTCH2NLB Human)

Use

Lentiviral

Tags

6xmyc tag

Expression

Mutation

deletion of C terminus from full length NOTCH2NLB

Promoter

CAG promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-EGFP

Plasmid

#122956

Purpose

Lentiviral expression of NOTCH2NL delta C terminus under CAG promoter with EGFP expression

Depositor

Pierre Vanderhaeghen

Article

Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

Insert

NOTCH2NL-delta C terminus (NOTCH2NLB Human)

Use

Lentiviral

Tags

6xhis tag

Expression

Mutation

deletion of C terminus from full length NOTCH2NLB

Promoter

CAG promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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XZ079 (PB)EF1a-CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Plasmid

#233435

Purpose

PiggyBac donor plasmid with CCR6-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Use

Tags

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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LSM042 (PB)EF1a-AVPR-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Plasmid

#233426

Purpose

PiggyBac donor plasmid AVPR2-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

AVPR-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Use

Tags

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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Const.6 EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc-PS9

Plasmid

#231910

Purpose

For packaging EGFP, dTomato, TagBFP and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-rtTA-IRES-nls-EGFP-WPRE-bGH-pA (JDW 410)

Plasmid

#229807

Purpose

A CAGGS driven tet-on transactivator with an nls-EGFP reporter

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

rtTA-IRES-nls-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-nls-mCherry-stop-IRES-myr-EGFP (JDW 1326)

Plasmid

#229812

Purpose

A CAGGS driven expression vector containing an nls-mCherry followed by an IRES and then a myristoylated EGFP for labeling the cell membrane.

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

nls-mCherry

Use

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-Vhh-LaminB-mNeonGreen-IRES-myr-BFP (JDW 1353)

Plasmid

#229845

Purpose

A CAGGS driven expression vector containing a vhh-Lamin nanobody for nuclear lamina labeling follwed by and IRES and a myristoylated TagBFP for labeling the cell membrane.

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Lamin nanobody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-MYC(Δ1-143; Δ340-439)-GFP-IRES-mCherry (ΔTADΔDBD)

Plasmid

#231235

Purpose

MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) and DNA-binding-domain (residues 340-439) for transient expression in mammalian cells

Depositor

Michael Rape

Article

Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

Insert

MYC (MYC Human)

Use

Tags

Expression

Mammalian

Mutation

TAD deleted (delta1-143), DBD deleted (delta340-4…

Promoter

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100)-IRES-puromycin

Plasmid

#223965

Purpose

Dual fluorescent reporter for histone H2B and for Cdt1

Depositor

Hee Won Yang

Article

Zhang et al Nat Commun. 2023 Nov 29;14(1):7847. doi:

Insert

H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100) (H2B, CDT1 Human)

Use

Lentiviral

Tags

iRFP670 and mCerulean

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-miRFP680-NES-p2A-ezrin-T567A-mRuby3-IRES-Neo

Plasmid

#222637

Purpose

Ezrin activation reporter, T567 phosphorylation deficient

Depositor

Arnold Hayer

Article

Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

Insert

miRFP680-NES-p2A-ezrin-T567A-mRuby3 (EZR Human)

Use

Lentiviral

Tags

mRuby3

Expression

Mutation

T567A

Promoter

EF1a

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC msNLRP3(LRRm5)-APEX2-3xFLAG-IRES-eGFP (H781E_Q782R_F785A)

Plasmid

#218636

Purpose

Proximity biotinylation of msNLRP3(LRRm5, H781E_Q782R_F785A)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

H781E, Q782R, F785A

Promoter

UBC

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC msNLRP3(LRRm2)-APEX2-3xFLAG-IRES-eGFP (N1008R_R1009E_E1010R_R1013E)

Plasmid

#218635

Purpose

Proximity biotinylation of msNLRP3(LRRm2, N1008R_R1009E_E1010R_R1013E)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

N1008R, R1009E, E1010R, R1013E

Promoter

UBC

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pmCherry2-GNB1-T2A-mCherry2-GNG2-IRES-GNAI1-mEYFP(Q69K)

Plasmid

#190755

Purpose

Expression of trimeric G protein with mCherry2 (GNB1 and GNG2) or mEYFP(Q69K) (GNAI1) tags.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Inserts

GNB1 (GNB1 Human)
GNG2 (GNG2 Human)
GNAI1 (GNAI1 Human)

Use

Tags

mCherry2 and mEYFP(Q69K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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