We narrowed to 3,959 results for: LYC

1. pGEX2T-TUBG1

   Plasmid

   #171967

   Purpose

   pGEX2T-TUBG1 bacterial expression plasmid for production of recombinant GST fusion protein

   Depositor

   Maria Alvarado-Kristensson

   Article

   Corvaisier et al Commun Biol. 2021 Jun 22;4(1):767. doi:

   Insert

   TUBG1 (TUBG1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   R275H (Please see depositor comments)

   Promoter

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. LENTICRISPR-HNRNPD_sgRNA1

   Plasmid

   #201598

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   HNRNPD (HNRNPD Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. LAMP1-bio-His

   Plasmid

   #51809

   Purpose

   Expresses full-length Lysosome-associated membrane glycoprotein 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   LAMP1 (LAMP1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. LENTICRISPR-ALDOA_sgRNA2

   Plasmid

   #201591

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

   Insert

   ALDOA (ALDOA Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFPC2-gephyrin S268A/270A

   Plasmid

   #199608

   Purpose

   Encodes N-terminal eGFP-tagged P1-gephyrin S268/270A for expression in mammalian cells.

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S268A/S270A

   Promoter

   CMV

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. GP1BA-bio-His

   Plasmid

   #51907

   Purpose

   Expresses full-length Platelet glycoprotein Ib alpha chain precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   GP1BA (GP1BA Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Flag-Avi-ires-CHD5

   Plasmid

   #68878

   Purpose

   Transient mammalian expression of CHD5 for BirA tagging

   Depositor

   Adrian Bracken

   Article

   Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

   Insert

   CHD5 (CHD5 Human)
   

   Use

   Tags

   FLAG/AVI

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pCDH-puro/Flag PD-L1 N35Q

   Plasmid

   #121449

   Purpose

   expression of human PD-L1 mutant in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   CD274 (CD274 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N35Q

   Promoter

   CMV

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR3-FLAG-Gephyrin E

   Plasmid

   #68819

   Purpose

   Flag tagged mammalian expression of E domain

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   internal EcoRI site has silent mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pUDP044

    Plasmid

    #101168

    Purpose

    pUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Insert

    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-GST-CHD5(Chromos)-FRAG

    Plasmid

    #68875

    Purpose

    IPTG inducible bacterial expression of CHD5 fragment

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    only the two chromodomains of CHD5

    Promoter

    tac

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCMV-FLAG-CHD5-WT-331-672aa

    Plasmid

    #68870

    Purpose

    Mammalian expression of CHD5 histone binding domain (331-672aa)

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    331-672aa

    Promoter

    CMV

    Available since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

13. GP1BBm-bio-His

    Plasmid

    #52314

    Purpose

    Expresses full-length Platelet glycoprotein Ib beta chain precursor monomer ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    GP1BBm (GP1BB Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. GP1BAm-bio-His

    Plasmid

    #53344

    Purpose

    Expresses full-length Platelet glycoprotein Ib alpha chain precursor monomer ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    P07359 (GP1BA Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV-FFluc-GPD1L-3'UTR Full mut

    Plasmid

    #32489

    Depositor

    Pere Puigserver

    Article

    Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

    Insert

    GPD1L 3'UTR (Gpd1l Mouse)
    

    Use

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutated in the entire miR-210 binding region

    Promoter

    CMV

    Available since

    Nov. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pPB-cT3G-cERP2-HHEX

    Plasmid

    #222928

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of HHEX

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    HHEX (HHEX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV5 n-myc mPirh2

    Plasmid

    #212994

    Purpose

    expresses mouse PIRH2

    Depositor

    Sheng-cai Lin

    Article

    Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

    Insert

    pirh2 (Rchy1 Mouse)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Stx5L(m55V)-GFP

    Plasmid

    #209845

    Purpose

    Expresses Stx5 M55V mutant fused to GFP in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    STX5 (STX5 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Changed Methionine 55 to Valine, causes loss of S…

    Promoter

    CMV

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-EFNB2-RBD-Fc (D62Q)

    Plasmid

    #200979

    Purpose

    Mammalian expression plasmid for soluble EFNB2 (D62Q specificity mutant) fused to IgG1 Fc

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EFNB2 (EFNB2 Human)
    

    Use

    Tags

    IgG1 Fc

    Expression

    Mammalian

    Mutation

    Soluble Receptor-Binding Domain; D62Q mutation fo…

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCEP4-myc-EFNB2 (D62Q)

    Plasmid

    #200976

    Purpose

    Mammalian expression plasmid for myc-tagged EFNB2 (D62Q specificity mutant)

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EFNB2 (EFNB2 Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    D62Q (increases specificity towards henipaviral G…

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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