We narrowed to 7,293 results for: ef1a

1. pcDNA5FRT-EF-Pdgfrbeta-EGFPN

   Plasmid

   #66790

   Purpose

   Expression of murine PDGFRbeta tagged with GFP under the control of EF1a promoter

   Depositor

   Lotte Pedersen

   Article

   Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

   Insert

   PDGFRbeta (Pdgfrb Mouse)
   

   Use

   Tags

   C-term EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. MCP-NMS

   Plasmid

   #210705

   Purpose

   This plasmid express EF1a promoter driven MCP-NMS transactivation module

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   MCP fused to transactivation domain from Human transcription factor NRF2, MRTF-A and STAT1
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N55K in MCP

   Promoter

   EF1a

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCTIP2-N174

   Plasmid

   #60858

   Purpose

   Expresses CTIP2 under the EF1a promoter

   Depositor

   Andrew Yoo

   Article

   Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

   Insert

   CTIP2 isoform b (Bcl11b Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-mCherry-hCdt1(1-100)ΔCy

   Plasmid

   #193759

   Purpose

   Expresses N-CRL4Cdt2 reporter(mCherry-hCdt1(1-100)ΔCy)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   mCherry-hCdt1(1-100)ΔCy (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

   Promoter

   EF1a

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLL8 lenti-D10Acas9-3xflag-blast

   Plasmid

   #112134

   Purpose

   lenti vector encoding D10Acas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-D10ACas9-3Xflag-T2A-Blast-WPRE)

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   D10Acas9-3xflag-blast
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A mutant in Cas9

   Promoter

   EF1a

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-mTurquoise-MLC-IRES-neo

   Plasmid

   #85145

   Purpose

   lentiviral expression of myosin light chain (MLC, MYL9), N-terminally tagged with mTurquoise

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   mTurquoise-MLC (myosin regulatory light chain, N-terminally tagged with mTurquoise (MYL9 Human)
   

   Use

   Lentiviral

   Tags

   mTurquoise

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-IV-GFPL10

   Plasmid

   #98704

   Purpose

   Cre-dependently express GFPL10 fusion using Introvert Switch

   Depositor

   Alexander Nectow, Jeffrey Friedman

   Article

   Nectow et al Cell. 2017 Jul 27;170(3):429-442.e11. do

   Insert

   GFPL10
   

   Use

   AAV and Cre/Lox

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   L10: 2609 C->T to remove EcoRI site

   Promoter

   EF1a

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. crTet in pSLQ8453

   Plasmid

   #183965

   Purpose

   U6-crTet-EF1a-Puro-T2A-BFP-WPRE

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Insert

   crTet
   

   Use

   Lentiviral

   Tags

   EF1a-Puro-T2A-BFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. MCP-3x9aaTAD

   Plasmid

   #210707

   Purpose

   This Plasmid express EF1a promoter driven MCP-3x 9aa transactivation module

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   MCP fused to 3x 9aa transactivation domain derived from MRTF-B and MYOCD1
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N55K in MCP

   Promoter

   EF1a

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-puro-N-Flag-Snrnp40

    Plasmid

    #134253

    Purpose

    Lentivector encoding Flag-tagged Snrnp40

    Depositor

    Bruce Beutler

    Article

    Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

    Insert

    Snrp40 (Snrnp40 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PB-iRFP-STOP-ReNL

    Plasmid

    #113965

    Purpose

    Piggybac transposon plasmid CRISPR gene deletion activatable fluorescence. Constitutive iRFP670 under EF1A promoter, CMV promoter with two SV40 polyA followed by red-enhanced nanolantern (ReNL)

    Depositor

    Jordan Green

    Article

    Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

    Inserts

    iRFP670
    ReNL
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV - SV40-PolyA - SV40-PolyA and EF1A

    Available since

    Dec. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti NbALFA-miRFP680 hygro

    Plasmid

    #193969

    Purpose

    lentivector coding for miRFP680 fused to a nanobody targeting the ALFA tag, hygromycin resistance

    Depositor

    Raphael Gaudin

    Insert

    NbALFA-miRFP680
    

    Use

    Lentiviral

    Tags

    miRFP680

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti NbALFA-miRFP680 puro

    Plasmid

    #193972

    Purpose

    lentivector coding for miRFP680 fused to a nanobody targeting the ALFA tag, puromycin resistance

    Depositor

    Raphael Gaudin

    Insert

    NbALFA-miRFP680
    

    Use

    Lentiviral

    Tags

    miRFP680

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Lenti NbALFA-mNeonGreen puro

    Plasmid

    #193973

    Purpose

    lentivector coding for mNeonGreen fused to a nanobody targeting the ALFA tag, puromycin resistance

    Depositor

    Raphael Gaudin

    Insert

    NbALFA-mNeonGreen
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Lenti NbALFA-mNeonGreen hygro

    Plasmid

    #193974

    Purpose

    lentivector coding for mNeonGreen fused to a nanobody targeting the ALFA tag, hygromycin resistance

    Depositor

    Raphael Gaudin

    Insert

    NbALFA-mNeonGreen
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. ER-P-TAG (pGS594)

    Plasmid

    #160039

    Purpose

    pLV-A4-UCOE-EF1a-CP450TMD-APEX2-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2

    Depositor

    David Sabatini

    Insert

    CP450TMD-APEX2-3xHA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDY0551-Luciferase RADARS

    Plasmid

    #182538

    Purpose

    Mammalian cell expression plasmid of Gaussia luciferase RADARS in a dual luciferase expression back bone with Cypridina luciferase as the control gene.

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Insert

    RADARS Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. piggyBAC-U6-GFP-WPRE

    Plasmid

    #200030

    Purpose

    piggyBAC vector backbone with GFP for cloning DNA Ticker Tapes and U6 for pegRNAs

    Depositor

    Jay Shendure

    Type

    Empty backbone

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. CROP-seq-opti-dsRed

    Plasmid

    #201999

    Purpose

    Based on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.

    Depositor

    Daniel Geschwind

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    dsRed

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. TK4-EGFP

    Plasmid

    #239047

    Purpose

    PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation

    Depositor

    Marius Wernig

    Inserts

    EGFP
    puroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and EF1a

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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