We narrowed to 11,784 results for: emb

Showing: 2001 - 2020 of 11784 results

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FLAG-3C-sGFP1-10_pcDNA3.1(-)

Plasmid

#182931

Purpose

For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment

Depositor

William Weis

Publication

Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

Type

Empty backbone

Tags

3C-cleavable split GFP 1-10, FLAG tag, and HA sig…

Expression

Mammalian

Promoter

CMV

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCDNA5/mCherry_pBirA-HA_FKBP8

Plasmid

#154457

Purpose

expresses mCherry_pBirA-HA_FKBP8/biochemical assay

Depositor

Hyun-Woo Rhee

Publication

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

mCherry_pBirA-HA_FKBP8 (FKBP8 Human)

Expression

Mammalian

Promoter

CMV-F

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGASΔN-HA

Plasmid

#130912

Purpose

Expresses human cGASΔN (aa160-522)-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Publication

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGASΔN

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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LV 1-5

Plasmid

#68411

Purpose

Lentiviral backbone for use with GMAP

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Type

Empty backbone

Use

Lentiviral; Gmap

Expression

Mammalian

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDD428

Plasmid

#202081

Purpose

Cas9/sgRNA plasmid targeting the C-terminus of Myh9

Depositor

Daniel Dickinson

Publication

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Myh9 CT sgRNA (Myh9 S. pyogenes (Cas9))

Use

CRISPR

Promoter

EF1a

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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OMM-V5-LOV-Turbo

Plasmid

#199665

Purpose

expresses LOV-Turbo on the mammalian outer mitochondrial membrane, lentiviral vector

Depositor

Alice Ting

Publication

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

AKAP1 transmembrane domain and V5

Expression

Mammalian

Promoter

TRE3G

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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FKBP-GFP-Sec61β

Plasmid

#172442

Purpose

Expression of ER protein Sec61beta with an N-terminal FKBP-GFP tag

Depositor

Stephen Royle

Publication

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Protein transport protein Sec61 subunit beta

Tags

FKBP-GFP

Expression

Mammalian

Promoter

CMV

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFP.E227

Plasmid

#138249

Purpose

Reporter plasmid - Expresses three fluorescent reporters from three different ECF promoters

Depositor

Baojun Wang

Publication

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

mCherry
Terminator
mTagBFP
Terminator
gfp
Terminator

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P17_up1691, P20_992, and iP16_3622

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3 AIF-mScarlet

Plasmid

#129576

Purpose

Expression of mScarlet fused AIF

Depositor

Stephen Tait

Publication

Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

Insert

AIF (AIFM1 Human)

Expression

Mammalian

Mutation

None

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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TOPO tTR-KRAB-rtTA3-Luc

Plasmid

#68449

Purpose

TOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

tTR-KRAB-2A-rtTA3-2A-Luciferase

Use

Luciferase; Gmap

Expression

Bacterial

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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ECFP(W66A)-FRB-MoA

Plasmid

#67904

Purpose

Expresses a Mitochondria located FRB domain in mammalian cells (the ECFP is not fluorescent)

Depositor

Dorus Gadella

Publication

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

ECFP(W66A)-FRB-MoA

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSLIK-Hygro-Myc-hAgo2

Plasmid

#60234

Purpose

Express HA-tagged wild-type Ago2 in mammalian cells in a Dox-inducible manner

Depositor

Zissimos Mourelatos

Publication

Liu et al Mol Cell. 2012 May 25;46(4):507-17. doi:

Insert

Argonaute2 (AGO2 Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available since

Nov. 12, 2014

Availability

Academic Institutions and Nonprofits only
pTK51_GFP-NuMA-C

Plasmid

#46349

Depositor

Iain Cheeseman

Publication

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

NuMA C-terminal fragment (NUMA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

C-terminal fragment from 1700-2115

Promoter

pBABE

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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Dynamin I-K44A-non-tagged

Plasmid

#32695

Depositor

Robert Lefkowitz

Publication

Ahn et al J Biol Chem. 2002 Jul 19;277(29):26642-5

Insert

Dynamin I (Dnm1 Rat)

Expression

Mammalian

Mutation

K44A

Promoter

CMV

Available since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
rEc2

Bacterial strain

#51946

Purpose

oriT-kan template

Depositor

Farren Isaacs

Publication

Ma et al Nat Protoc. 2014 Oct;9(10):2285-300. doi

Bacterial resistance

Kanamycin

Available since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCDNA5/avitag-apex2-v5-fkbp8

Plasmid

#154460

Purpose

expresses avitag-apex2-v5-fkbp8/biochemical assay

Depositor

Hyun-Woo Rhee

Publication

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

avitag-apex2-v5-fkbp8 (FKBP8 Human)

Expression

Mammalian

Promoter

CMV-F, T7 promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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Gata3-T2A-H2B-eGFP donor

Plasmid

#113119

Purpose

Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Gata3 coding sequence

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Gata3 donor region including homology arms and T2A-H2B-eGFP

Use

Synthetic Biology

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Ctcf-Halo-mAID donor

Plasmid

#113103

Purpose

Donor plasmid for knock-in mAID-Halo fusion to the c-terminal of mouse Ctcf coding sequence

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

c terminal Ctcf donor region including homology arms and mAID-HaloTag (Ctcf Synthetic, Mouse)

Use

Synthetic Biology

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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R4227X-F

Plasmid

#21371

Depositor

Gregory Germino

Publication

Hanaoka et al Nature. . 408(6815):990-4.

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Tags

FLAG (following D4226)

Expression

Mammalian

Mutation

Changed R4227 to stop codon

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
3xDBE_RE:NLuc

Plasmid

#178318

Purpose

FOXO NLuc luciferase reporter

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

FOXO NLuc Reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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