Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 8,813 results for: Ott

Showing: 5051 - 5100 of 8813 results

  1. pUC57-mini v2-HF24_F13

    Plasmid
    #195727
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#13 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195727-plasmid-map-sequence-id-385601

    Enlarge

  2. pUC57-mini v2-HF24_F22

    Plasmid
    #195736
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#22 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195736-plasmid-map-sequence-id-385599

    Enlarge

  3. pUC57-mini v2-HF24_F10

    Plasmid
    #195724
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#10 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195724-plasmid-map-sequence-id-385596

    Enlarge

  4. pUC57-mini v2-HF24_F6

    Plasmid
    #195720
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#6 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195720-plasmid-map-sequence-id-385595

    Enlarge

  5. pUC57-mini v2-HF24_F15

    Plasmid
    #195729
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#15 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195729-plasmid-map-sequence-id-385594

    Enlarge

  6. pUC57-mini v2-HF24_F17

    Plasmid
    #195731
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#17 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195731-plasmid-map-sequence-id-385593

    Enlarge

  7. pUC57-mini v2-HF24_F19

    Plasmid
    #195733
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#19 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195733-plasmid-map-sequence-id-385592

    Enlarge

  8. pUC57-mini v2-HF24_F2

    Plasmid
    #195716
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#2 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195716-plasmid-map-sequence-id-385591

    Enlarge

  9. pUC57-mini v2-HF24_F11

    Plasmid
    #195725
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#11 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195725-plasmid-map-sequence-id-385589

    Enlarge

  10. pUC57-mini v2-HF24_F16

    Plasmid
    #195730
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#16 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195730-plasmid-map-sequence-id-385583

    Enlarge

  11. pUC57-mini v2-HF24_F18

    Plasmid
    #195732
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#18 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195732-plasmid-map-sequence-id-385582

    Enlarge

  12. pUC57-mini v2-HF24_F1

    Plasmid
    #195715
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#1 of a 24 fragment split in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195715-plasmid-map-sequence-id-385580

    Enlarge

  13. pUC57-mini v2-HF24_F9

    Plasmid
    #195723
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#9 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195723-plasmid-map-sequence-id-385578

    Enlarge

  14. pUC57-mini v2-HF24_F23

    Plasmid
    #195737
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#23 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195737-plasmid-map-sequence-id-385577

    Enlarge

  15. pUC57-mini v2-HF24_F21

    Plasmid
    #195735
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#21 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195735-plasmid-map-sequence-id-385575

    Enlarge

  25. pJCC_163 SpCas9 K233A/K234A/K253A/K263A

    Plasmid
    #179527
    Purpose
    For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag
    Depositor
    Jennifer Doudna
    Article
    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-
    Insert
    SpCas9 K233A/K234A/K253A/K263A
    Use
    Tags
    10X His, TEV protease cleavage site, and maltose-…
    Expression
    Bacterial
    Mutation
    changed lysines 233, 234, 253, and 263 to alanine
    Promoter
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    179527-plasmid-map-sequence-id-351733

    Enlarge

  26. pPOLY_new

    Plasmid
    #179276
    Purpose
    Expresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d
    Inserts
    cellodextrin phosphorylase
    cellobiose phosphorylase
    sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)
    Use
    Synthetic Biology
    Tags
    6xHIS and Strep-Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lacO
    Available since
    April 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    179276-plasmid-map-sequence-id-358730

    Enlarge

  27. pBICI_high ori

    Plasmid
    #179273
    Purpose
    Expresses CuCBP and BaSP in E. coli BL21(DE3)
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lacO
    Available since
    April 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    179273-plasmid-map-sequence-id-358714

    Enlarge

  28. pDUBI

    Plasmid
    #179274
    Purpose
    Expresses CcCDP monocistronically and CuCBP and BaSP bicistronically
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d
    Inserts
    cellodextrin phosphorylase
    cellobiose phosphorylase
    C8077_RS00435 sucrose phosphorylase [ Bifidobacterium adolescentis (C8077_RS00435 Bifidobacterium alodescentis)
    Use
    Synthetic Biology
    Tags
    6xHIS and Strep-Tag II
    Expression
    Bacterial
    Mutation
    sucrose phosphorylase
    Promoter
    T7 lacO
    Available since
    April 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    179274-plasmid-map-sequence-id-358736

    Enlarge

  29. p406 – TDH3-ER-FlucDM-GFP11-Ura

    Plasmid
    #177731
    Purpose
    DM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus in yeast plasmid with
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    FlucDM
    Use
    Tags
    GFP11, HA, HDEL, and KAR2 Signal Sequence
    Expression
    Bacterial and Yeast
    Mutation
    R188Q, R261Q (Destabilized Mutant)
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177731-plasmid-map-sequence-id-352614

    Enlarge

  31. CMV-10xMS2 no wild type

    Plasmid
    #158204
    Purpose
    Cassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted form the oligo pool experiment. It does not contain the MS2-wt sequence, therefore QCP cannot bind it.
    Depositor
    Roee Amit
    Article
    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:
    Insert
    10 binding sites of MS2 without the WT sequence from oligo library experiment
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    158204-plasmid-map-sequence-id-311816

    Enlarge

  32. CMV-10xQb no wild type

    Plasmid
    #158205
    Purpose
    Cassette of 10 different binding sites with high affinity to Qb phage coat protein, as extracted form the oligo pool experiment. It does not contain the Qb-wt sequence, therefore MCP cannot bind it.
    Depositor
    Roee Amit
    Article
    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:
    Insert
    10 binding sites of QB coat protein without the WT sequence based on the oligo library experiment
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    158205-plasmid-map-sequence-id-311817

    Enlarge

  40. pMS22

    Plasmid
    #129710
    Purpose
    Expresses Escherichia coli RssB E134G:E135S:E136G:E137S variant as an N-terminal 10His fusion
    Depositor
    Alexandra Deaconescu
    Article
    Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.
    Insert
    Escherichia coli RssB-GSGS variant (rssB Escherichia coli MG1655)
    Use
    Tags
    10-Histidine tag cleavable with PreScission Prote…
    Expression
    Bacterial
    Mutation
    RssB E134G:E135S:E136G:E137S
    Promoter
    T7
    Available since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    129710-plasmid-map-sequence-id-254924

    Enlarge

  41. KL001: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-VPR

    Plasmid
    #121836
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS/VPR scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121836-plasmid-map-sequence-id-237390

    Enlarge

  42. KL101: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-KRAB

    Plasmid
    #121837
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS/KRAB scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121837-plasmid-map-sequence-id-237391

    Enlarge

  43. KL202: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-LSD1

    Plasmid
    #121838
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS/LSD1 scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121838-plasmid-map-sequence-id-237392

    Enlarge

  44. KL401: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-Dnmt3a3L

    Plasmid
    #121840
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS/Dnmt3a-3L scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121840-plasmid-map-sequence-id-237394

    Enlarge

  45. JN511: pMVP (L3-L2) pA; EF1a::eGFP-P2A-TETa-pA

    Plasmid
    #121803
    Purpose
    pMVP L3-L2 entry plasmid, contains polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven GFP-P2A-TETa downstream of gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    polyA + EF1a::eGFP-P2A-TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121803-plasmid-map-sequence-id-237312

    Enlarge

  46. IG806: pMVP (L3-L2) pA; RIP::eGFP-P2A-TETa-pA

    Plasmid
    #121807
    Purpose
    pMVP L3-L2 entry plasmid, contains polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven GFP-P2A-TETa downstream of gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    polyA + RIP::eGFP-P2A-TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121807-plasmid-map-sequence-id-237316

    Enlarge

  47. LF901: pMAGIC (L1-R5) mU6::SaCas9 gRNA scaffold

    Plasmid
    #121811
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121811-plasmid-map-sequence-id-237356

    Enlarge

  48. JI601: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; RIP

    Plasmid
    #121815
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121815-plasmid-map-sequence-id-237362

    Enlarge

  49. JI702: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; RIP

    Plasmid
    #121820
    Purpose
    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121820-plasmid-map-sequence-id-237373

    Enlarge

Showing: 5051 - 5100 of 8813 results