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HA-UBE2E1 WT in pcDNA3.1

Plasmid

#118213

Purpose

Expresses HA-UBE2E1 in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

UBE2E1 (UBE2E1 Human)

Tags

HA

Expression

Mammalian

Mutation

none

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd3

Plasmid

#42255

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 3 (Fzd3 Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
Cry2L348F(1-531)-mCh-BAXS184E

Plasmid

#117239

Purpose

Light activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switch

Depositor

Robert Hughes

Article

Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

Insert

Cry2531.L348F-mCh-BAXS184E

Expression

Mammalian

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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Cry2(1-531)-mCh-BAXS184E

Plasmid

#117238

Purpose

Light activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switch

Depositor

Robert Hughes

Article

Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

Insert

Cry2531-mCh-BAXS184E

Expression

Mammalian

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hRabin8 F201A

Plasmid

#26728

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Rabin8 (RAB3IP Human)

Tags

EGFP

Expression

Mammalian

Mutation

F201A: GEF domain mutant

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-hRabin8 L196A

Plasmid

#26727

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Rabin8 (RAB3IP Human)

Tags

EGFP

Expression

Mammalian

Mutation

L196A: GEF domain mutant.

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only
pENN.AAV.CB7.CI.PM20D1-flag.WPRE.rBG

Plasmid

#132682

Purpose

AAV plasmid encoding mouse PM20D1 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Peptidase M20 domain containing 1 (Pm20d1 Mouse)

Use

AAV

Tags

6xHIS and Flag

Promoter

Chicken b-actin

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLN429 (FuGW-G8p-mKate2)

Plasmid

#105185

Purpose

Module 3 - GAL4 synthetic promoter ( with eight binding sites) drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CB7.CI.PM20D2-flag.WPRE.rBG

Plasmid

#132684

Purpose

AAV plasmid encoding mouse PM20D2 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Peptidase M20 domain containing 2 (Pm20d2 Mouse)

Use

AAV

Tags

Flag

Promoter

Chicken b-actin

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pLN426 (FuGW-G5p-mKate2)

Plasmid

#105183

Purpose

Module 3 - GAL4 synthetic promoter (with five binding sites) drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G5p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-cfRabin8_4

Plasmid

#26713

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rabin8 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

RNAi to dog Rabin8 alpha isoform.

Available Since

Dec. 14, 2010

Availability

Academic Institutions and Nonprofits only
pLN432 (FuGW-G14p-mKate2)

Plasmid

#105187

Purpose

Module 3 - GAL4 synthetic promoter( with fourteen binding sites) drives mKate2

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G14p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pAAV- GfaABC1D::Cas9-HA-sgRNA-Ezr-seq2

Plasmid

#240865

Purpose

GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of sgRNA targeting exon-1 of mouse Ezrin; for knocking-out ezrin in murine astrocytes

Depositor

Mark Verheijen

Article

Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23

Insert

U6 driven sgRNA Targeting exon 1 of EZR (Ezr Mouse)

Use

AAV and CRISPR

Tags

SaCas9 + 3X HA-Tag

Expression

Mammalian

Promoter

GfaABC1D

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV- GfaABC1D::Cas9-HA-sgRNA-Ezr-Seq1

Plasmid

#240094

Purpose

GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of sgRNA targeting exon-1 of mouse Ezrin; for knocking-out ezrin in murine astrocytes

Depositor

Mark Verheijen

Article

Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23

Insert

U6 driven sgRNA Targeting exon 1 of EZR (Ezr Mouse)

Use

AAV and CRISPR

Tags

SaCas9 + 3X HA-Tag

Expression

Mammalian

Promoter

GfaABC1D

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83-GB1_AD

Plasmid

#148514

Purpose

Bacterial Expression of DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83 (Thor Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-675-GB1_AD

Plasmid

#148515

Purpose

Bacterial Expression of DmThor_50-63-DmeIF4Ga_631-675

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmThor_50-63-DmeIF4Ga_631-675 (Thor Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-ChrimsonR-tdT

Plasmid

#59169

Purpose

Expression vector for ChrimsonR-tdTomato

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-tdTomato

Tags

tdTomato

Expression

Mammalian

Mutation

K176R

Promoter

CAG

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ChrimsonR-GFP

Plasmid

#58806

Purpose

AAV expression of ChrimsonR-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

K176R

Promoter

Syn

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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FCK-ChrimsonR-GFP

Plasmid

#59049

Purpose

Lentiviral expression of ChrimsonR under the CaMKII promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-GFP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

K176R

Promoter

CaMKII

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pcmv3-STING-C1471S-FLAG

Plasmid

#198188

Purpose

Mammalian expression of FLAG-tagged mouse STING1 C1471S

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

TMEM173 (Sting1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

The cysteine in STING at position 147 mutates to …

Promoter

CMV

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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