We narrowed to 2,475 results for: T2A

1. pYPQ-ABE-SpRYn-Act3.0

   Plasmid

   #178959

   Purpose

   It consists of a SpRY nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Insert

   ABE8e-SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pY31

   Plasmid

   #84746

   Purpose

   Expresses huLbCpf1-P2A-puro and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huLbCpf1
   puromycin resistance gene
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCC324

   Plasmid

   #110165

   Purpose

   miniMos vector expresses ERKKTR-mClover and mCherry-H2B in C. elegans VPCs; request with plasmids pCC335 and pCC336 for unphosphorylated and phosphomimetic controls (Contains Neomycin resistance)

   Depositor

   Iva Greenwald

   Article

   de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

   Insert

   ERK-KTR-mClover-T2A-mCherry-H2B
   

   Use

   Minimos transposon

   Tags

   mCherry and mClover

   Expression

   Worm

   Mutation

   R61K change made in ERK-KTR CDS

   Promoter

   lin-31 promoter/enhancer

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. lenti-HA-mBec1 F121A

   Plasmid

   #99506

   Purpose

   Lentiviral expression of mutant Becn1 leading to hyperactive autophagy

   Depositor

   Congcong He

   Article

   Rocchi et al PLoS Genet. 2017 Aug 14;13(8):e1006962.

   Insert

   Beclin 1 (Becn1 Mouse)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   F121A

   Promoter

   CMV

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAX198

   Plasmid

   #173042

   Purpose

   Custom vector for sgRNA library construction

   Depositor

   Brittany Adamson

   Article

   Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

   Insert

   HBB Gene - Second and third exons of ENST00000647020.1 (no intron) and part of the 3' UTR (HBB Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT

   Plasmid

   #216763

   Purpose

   PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposase

   Depositor

   Alex Dunn

   Article

   Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

   Insert

   mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
   

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Available Since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCyCEN_Lisp2mCherry_hsp70_GFP

   Plasmid

   #137169

   Purpose

   Dual fluorescent reporter for liver stage expression in Plasmodium cynomolgi

   Depositor

   Clemens Kocken

   Article

   Voorberg-van der Wel et al Commun Biol. 2020 Jan 3;3:7. doi: 10.103

   Inserts

   mCherry
   Nanoluc
   dihydrofolate reductase
   Green Fluorescent Protein
   

   Use

   Unspecified

   Tags

   T2A

   Promoter

   P. cynomolgi hsp70 and P. cynomolgi lisp2

   Available Since

   May 11, 2020

   Availability

   Academic Institutions and Nonprofits only

8. AAVS1 KanR foundation lox casette

   Plasmid

   #132388

   Purpose

   Foundation cassette containing lox sites for cre-mediated exchange of inducible vectors, confers mammalian G418 resistance, targets AAVS1 locus

   Depositor

   Gerold Schmitt-Ulms

   Article

   Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

   Insert

   G418 resistance
   

   Use

   CRISPR and Cre/Lox

   Expression

   Mammalian

   Promoter

   promoterless

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. lenti-HA-mBec1

   Plasmid

   #99505

   Purpose

   3rd generation lentiviral expression of wild-type Becn1

   Depositor

   Congcong He

   Article

   Rocchi et al PLoS Genet. 2017 Aug 14;13(8):e1006962.

   Insert

   Beclin 1 (Becn1 Mouse)
   

   Use

   Lentiviral

   Tags

   HA

   Promoter

   CMV

   Available Since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Pblu-AAVS1-Cas9-p300-M2rtTA-AAVS1

    Plasmid

    #112261

    Purpose

    Donor plasmid with a Cas9-p300 expression cassette, a M2rtTA expression cassette, a T2A-puro selection cassette, and two gRNA recognition sequences at both ends of the whole insertion DNA fragment.

    Depositor

    Zhili Rong

    Article

    Ma et al FEBS Lett. 2018 Jun 5. doi: 10.1002/1873

    Insert

    spCas9-p300 (EP300 Human, Synthetic)
    

    Expression

    Mammalian

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9(BB)-2A-Neo_h53BP1_gRNA_D

    Plasmid

    #110300

    Purpose

    Expresssion of Cas9-T2A-neomycin resistant gene and a gRNA targeting exon 10 of human 53BP1

    Depositor

    Chris Kok-Lung Chan

    Article

    Addis Jones et al Nat Commun. 2019 Jun 28;10(1):2861. doi:

    Insert

    p53-binding protein 1 (TP53BP1 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB-CAG-Klf2.2A.Nanog-Cherry-pA-pgk-zeo

    Plasmid

    #74919

    Purpose

    Mammalian expression of mKlf2 and mNanog-mCherry

    Depositor

    Jose Silva

    Article

    dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

    Inserts

    Klf2 (Klf2 Mouse)
    Nanog (Nanog Mouse)
    

    Tags

    T2A and mCherry

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

13. PB-U6-CNCB_sgNANOG

    Plasmid

    #242176

    Purpose

    A piggybac-based vector containing mouse U6 promoter-driven NANOG sgRNA and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

    Depositor

    Kazutoshi Takahashi

    Article

    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

    Insert

    NANOG (NANOG Human)
    

    Use

    Piggybac

    Expression

    Mammalian

    Promoter

    mouse U6 promoter

    Available Since

    Oct. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNF-KB-Split PS Intein-C tTA

    Plasmid

    #242035

    Purpose

    C-terminal segment of a blue-light-controlled split PS Intein tTA gene expression system, co-expressing mCherry under an NF-KB-inducible promoter

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

    Insert

    Split PS Intein-C tTA
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    The CMV promoter in the original vector was repla…

    Promoter

    NF-KB

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-U6-CNCB_sgSOX2

    Plasmid

    #242175

    Purpose

    A piggybac-based vector containing mouse U6 promoter-driven SOX2 sgRNA and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

    Depositor

    Kazutoshi Takahashi

    Article

    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Piggybac

    Expression

    Mammalian

    Promoter

    mouse U6 promoter

    Available Since

    Sept. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-U6-CNCB_sgPOU5F1

    Plasmid

    #242174

    Purpose

    A piggybac-based vector containing mouse U6 promoter-driven POU5F1sgRNA and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

    Depositor

    Kazutoshi Takahashi

    Article

    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

    Insert

    POU5F1 (POU5F1 Human)
    

    Use

    Piggybac

    Expression

    Mammalian

    Promoter

    mouse U6 promoter

    Available Since

    Sept. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-HA-CBS(opt)-FLAG

    Plasmid

    #230088

    Purpose

    expressing human CBS

    Depositor

    Xingguo Zhu

    Article

    Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

    Insert

    CBS (CBS Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Multiple wobble mutations

    Promoter

    CMV

    Available Since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHPHS926

    Plasmid

    #228237

    Purpose

    Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible β-globin NMD reporter with premature termination codon for integration into Bxb1 attP_GA landing pad

    Depositor

    Arvind Rasi Subramaniam

    Article

    Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

    Inserts

    mCherry
    Beta globin
    

    Tags

    3xFLAG, HA, and PuroR

    Expression

    Mammalian

    Mutation

    PTC39

    Promoter

    TRE3GV and none

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-U6-E690_guide-CBh-hSpCas9

    Plasmid

    #188545

    Purpose

    Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid E690

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    T2A-EGFP

    Promoter

    U6

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330-U6-W308_guide-CBh-hSpCas9

    Plasmid

    #188546

    Purpose

    Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid W308

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    T2A-EGFP

    Promoter

    U6

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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