We narrowed to 2,779 results for: buc
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Plasmid#89906PurposeBacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.DepositorInsertCas13b
ExpressionBacterialPromoterLacAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1_RAF1 (51-131)
Plasmid#119218Purposepurification of GST-RAF1 RBD from E. coliDepositorInsertRAF1 aa 51-131 (RAF1 Human)
ExpressionBacterialAvailable SinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-SNAP-CD86iso1
Plasmid#223601PurposeLentiviral expression of human CD86 isoform 1 with SNAP tag in mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-Spy-CD86
Plasmid#223598PurposeLentiviral expression of human CD86 with SpyTag003 in mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-AmCyan
Plasmid#138481PurposeFor cloning sgRNA flanked by two ribozyme elements, to subsequently cloned into PL-5LTR-GW-A vector.DepositorInsertAmCyan
ExpressionMammalianAvailable SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
FKBP:FRB-FBAR
Plasmid#223620PurposeTransient expression of human FBP17 with mGFP, FRB, FKBP and TagBFP tags in mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-SNAP-PDL1
Plasmid#223602PurposeLentiviral expression of human PD-L1 with SNAP tag in mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CD80-mTFP1
Plasmid#223605PurposeLentiviral expression of human CD80 with mTFP1 tag in mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-mGFP-Eps15DN
Plasmid#223621PurposeTransient expression of human Eps15 with mGFP tag in mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CD86-mTFP1
Plasmid#223606PurposeLentiviral expression of human CD86 with mTFP1 tag in mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_ZCCHC7_PAX5
Plasmid#205958PurposeExpress mEGFP-tagged fusion protein, ZCCHC7_PAX5 from patient-derived sequenceDepositorAvailable SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(SMARCA2(47))-PGKpuro2ABFP-W
Plasmid#200510PurposeLentiviral vector expressing gRNA targeting human SMARCA2DepositorInsertSMARCA2(47) (SMARCA2 Human)
UseLentiviralAvailable SinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
YA1529: pAAV_hSyn-QuasAr3-P2A-CheRiff
Plasmid#107700Purposein vivo voltage imagingDepositorInsertQuasAr3-P2A-CheRiff
UseAAVExpressionMammalianPromoterhuman synapsin IAvailable SinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-3xFLAG-YAP1-S127A
Plasmid#42239DepositorInsertYAP1 (YAP1 Human)
UseGatewayTags3xFLAGMutationSerine 127 changed to alaninePromoternoneAvailable SinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
SGyA
Plasmid#160292PurposeDrives expression of Cas9 and a TdTomato fluorescent markerDepositorInsertsVas promoter
hSpCas9
tdTomato
CTCF insulator
Gypsy insulator
Left homology arm Y chromosome
Right homology arm Y chromosome
TagsT2A GFPExpressionInsectAvailable SinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag4A-MMP8 Wt
Plasmid#29545DepositorAvailable SinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP/CALR wt-FLAG
Plasmid#204525PurposeRetroviral expression of human CALR wt-FLAGDepositorInsertCALR wt-FLAG (CALR Human)
UseRetroviralAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO_Ollas.S.V5_mCherry-NLS
Plasmid#178211PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsNuclear Localization Signal and Ollas.S.V5PromotereF1aAvailable SinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/DEST/hCx43-E2HA-stop
Plasmid#40909DepositorInsertConnexin 43 internal HA tag (GJA1 Human)
ExpressionMammalianMutationHA tag inserted in second extracellular loopPromoterCMVAvailable SinceNov. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
PL-5LTR-GW-A
Plasmid#138480PurposeFor expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.DepositorInsertGateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes
ExpressionMammalianAvailable SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only