We narrowed to 3,263 results for: pET28

1. p360_DG

   Plasmid

   #234703

   Purpose

   Golden gate domesticated pET28b for scarless cloning with His10-mNeongreen-bdNEDD8

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   His10-mNeongreen-bdNEDD8

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p366_DG

   Plasmid

   #234687

   Purpose

   Golden gate domesticated pET28b with His10-bdNEDD8 and Avi tag

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   Avi tag and His10-bdNEDD8

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p371_DG

   Plasmid

   #234694

   Purpose

   Golden gate domesticated pET28b with Intein-Chitin binding domain

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   Intein-Chitin binding domain

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 8R6A

   Plasmid

   #228446

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Article

   Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

   Insert

   DTX1 (DTX1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. DTX2

   Plasmid

   #228447

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Article

   Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

   Insert

   DTX2 (DTX2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. RNF146

   Plasmid

   #228448

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Article

   Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

   Insert

   RNF146 (RNF146 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AF10_OM-LZ

   Plasmid

   #111753

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Article

   Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

   Insert

   MLLT10 (MLLT10 Human)
   

   Use

   Tags

   6x His - TEV

   Expression

   Bacterial

   Mutation

   Contains the OM-LZ region of AF10 (residues 720-7…

   Promoter

   T7

   Available since

   June 14, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. DOT1L-CC3

   Plasmid

   #111754

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Article

   Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

   Insert

   DOT1L (DOT1L Human)
   

   Use

   Tags

   8xHis-Thrombin-SUMO-TEV

   Expression

   Bacterial

   Mutation

   Contains coiled coil #3 (residues 613-661)

   Promoter

   T7

   Available since

   June 14, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. DOT1L-CC1

   Plasmid

   #111756

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Article

   Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

   Insert

   DOT1L (DOT1L Human)
   

   Use

   Tags

   8xHis-Thrombin-SUMO-TEV

   Expression

   Bacterial

   Mutation

   contains coiled coil #1 (residues 506-555)

   Promoter

   T7

   Available since

   June 14, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. DOT1L-CC2

    Plasmid

    #111755

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Article

    Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

    Insert

    DOT1L (DOT1L Human)
    

    Use

    Tags

    8xHis-Thrombin-SUMO-TEV

    Expression

    Bacterial

    Mutation

    Contains coiled coil #2 (residues 560-608)

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. AB-GFP

    Plasmid

    #72203

    Purpose

    screen AB42 variants for aggregation

    Depositor

    Michael Hecht

    Article

    Wurth et al J Mol Biol. 2002 Jun 21;319(5):1279-90.

    Insert

    AB42 (APP Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pZM3[I:FruR-L,I:RbsR-L]

    Plasmid

    #60777

    Purpose

    Contains PI driving expression FruR-L, and PI driving expression of RbsR-L.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Inserts

    FruR-L
    RbsR-L
    

    Use

    Tags

    Expression

    Mutation

    LacI/GalR repressor chimera. LacI DBD with FruR L…

    Promoter

    Available since

    July 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 3I91

    Plasmid

    #62514

    Purpose

    Bacterial expression plasmid for use in structural determination.

    Depositor

    Cheryl Arrowsmith

    Article

    Kaustov et al J Biol Chem. 2011 Jan 7;286(1):521-9. do

    Insert

    CBX8 (CBX8 Human)
    

    Use

    Tags

    6xHis and TEV cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. HOXB4 (human) HA-TAT-tag pET

    Plasmid

    #8526

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB4 (HOXB4 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    replace T7 and HIS tags of pET with an HA tag;

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

15. His 3C Kinase dead MKK6 1-334

    Plasmid

    #87882

    Purpose

    E. coli expression of MKK6 kinase dead

    Depositor

    Peter Mace

    Article

    Weijman et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    MKK6 (MAP2K6 Human)
    

    Use

    Tags

    3C protease site and 6xHis

    Expression

    Bacterial

    Mutation

    K82A, kinase dead mutant

    Promoter

    T7

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 6MIW

    Plasmid

    #127291

    Purpose

    Bacterial expression for structure determination. May not contain entire coding region of gene

    Depositor

    Cheryl Arrowsmith

    Article

    Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    6xHis-TEV (MHHHHHHSSGRENLYFQG)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. TRIP12-V2_A755-A841

    Plasmid

    #194759

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Article

    Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

    Insert

    TRIP12 (TRIP12 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 19, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pJK635

    Plasmid

    #72473

    Purpose

    pET28a modified to contain TEV protease fusion tag instead of T7 tag

    Depositor

    T. J. Kappock

    Type

    Empty backbone

    Use

    Tags

    His6 and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAM75

    Plasmid

    #224249

    Purpose

    pET28a MWG-ubiquitin-G77-C78

    Depositor

    Andreas Martin

    Article

    Xie et al Methods Mol Biol. 2023;2591:79-100. doi:

    Insert

    MWG-ubiquitin-G77-C78
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pH6-mNeon

    Plasmid

    #178460

    Purpose

    The mNeonGreen fluorescent protein with an N-terminal 6xHis-tag in pET28a.

    Depositor

    Siegfried Musser

    Article

    Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

    Insert

    mNeonGreen
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge