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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,289 results for: pcr

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Showing: 2021 - 2040 of 7289 results

  1. pAAH3

    Plasmid
    #41827
    Purpose
    Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for phleomycin resistance.
    Depositor
    Melissa Moore
    Publication
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Expression
    Yeast
    Promoter
    TEF
    Available since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    41827-plasmid-map-sequence-id-69168

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  4. pIRE4-Bpa

    Plasmid
    #155342
    Purpose
    Plasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.
    Depositor
    Irene Coin
    Publication
    Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15
    Insert
    humanized EBpaRS/BstYam
    Expression
    Mammalian
    Promoter
    PGK/U6
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    155342-plasmid-map-sequence-id-310519

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  5. pENTR221-sEFp-Blast-P2A-IKZF3d

    Plasmid
    #251485
    Purpose
    This vector serves as a PCR template to generate donor DNA that is used for knocking-in IKZF3d for conditional protein degradation. Short EF promoter enhances the expression of IKZF3d-tagged protein.
    Depositor
    Tomoharu Kanie
    Publication
    Liu et al bioRxiv 2026.05.19.726384
    Insert
    IKZF3d
    Use
    Synthetic Biology
    Promoter
    short EF Promoter
    Available since
    May 26, 2026
    Availability
    Academic Institutions and Nonprofits only
    251485-plasmid-map-sequence-id-501126

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  6. pENTR221-sEFp-Blast-P2A-HaloTag

    Plasmid
    #251486
    Purpose
    This vector serves as a PCR template to generate donor DNA that is used for knocking-in HaloTag for conditional protein degradation. Short EF promoter enhances the expression of Halo-tagged protein.
    Depositor
    Tomoharu Kanie
    Publication
    Liu et al bioRxiv 2026.05.19.726384
    Insert
    HaloTag7
    Use
    Synthetic Biology
    Promoter
    short EF Promoter
    Available since
    May 26, 2026
    Availability
    Academic Institutions and Nonprofits only
    251486-plasmid-map-sequence-id-501114

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  7. pENTR221-miniCMVp-Blast-P2A-dTAG

    Plasmid
    #251481
    Purpose
    This vector serves as a PCR template to generate donor DNA that is used for knocking-in dTAG for conditional protein degradation. MiniCMV promoter may enhance the expression of dTAG-tagged protein.
    Depositor
    Tomoharu Kanie
    Publication
    Liu et al bioRxiv 2026.05.19.726384
    Insert
    dTAG
    Use
    Synthetic Biology
    Promoter
    minimal CMV Promoter
    Available since
    May 26, 2026
    Availability
    Academic Institutions and Nonprofits only
    251481-plasmid-map-sequence-id-501112

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  8. pENTR221-miniCMVp-Blast-P2A-IKZF3d

    Plasmid
    #251482
    Purpose
    This vector serves as a PCR template to generate donor DNA that is used for knocking-in IKZF3d for conditional protein degradation. MiniCMV promoter may enhance the expression of IKZF3d-tagged protein
    Depositor
    Tomoharu Kanie
    Publication
    Liu et al bioRxiv 2026.05.19.726384
    Insert
    IKZF3d
    Use
    Synthetic Biology
    Promoter
    minimal CMV Promoter
    Available since
    May 26, 2026
    Availability
    Academic Institutions and Nonprofits only
    251482-plasmid-map-sequence-id-501111

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  9. pENTR221-miniCMVp-Blast-P2A-HaloTag

    Plasmid
    #251483
    Purpose
    This vector serves as a PCR template to generate donor DNA that is used for knocking-in HaloTag for conditional protein degradation. MiniCMV promoter may enhance the expression of Halo-tagged protein.
    Depositor
    Tomoharu Kanie
    Publication
    Liu et al bioRxiv 2026.05.19.726384
    Insert
    HaloTag7
    Use
    Synthetic Biology
    Promoter
    minimal CMV Promoter
    Available since
    May 26, 2026
    Availability
    Academic Institutions and Nonprofits only
    251483-plasmid-map-sequence-id-501113

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  10. pENTR221-sEFp-Blast-P2A-dTAG

    Plasmid
    #251484
    Purpose
    This vector serves as a PCR template to generate donor DNA that is used for knocking-in dTAG for conditional protein degradation. Short EF promoter enhances the expression of dTAG-tagged protein.
    Depositor
    Tomoharu Kanie
    Publication
    Liu et al bioRxiv 2026.05.19.726384
    Insert
    dTAG
    Use
    Synthetic Biology
    Promoter
    short EF Promoter
    Available since
    May 26, 2026
    Availability
    Academic Institutions and Nonprofits only
    251484-plasmid-map-sequence-id-501127

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  12. FFA1-Tango

    Plasmid
    #66280
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FFA1 (FFAR1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  15. S1PR2-Tango

    Plasmid
    #66497
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S1PR2 (S1PR2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    66497-plasmid-map-sequence-id-344626

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  18. GPRC5A-Tango

    Plasmid
    #66382
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPRC5A (GPRC5A Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    66382-plasmid-map-sequence-id-203070

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  20. pcDNA3.1-Beta3

    Plasmid
    #140988
    Purpose
    Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GBeta3 (GNB3 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    140988-plasmid-map-sequence-id-301591

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Showing: 2021 - 2040 of 7289 results