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pVC-Ds-DrU6a:sgRNA-com-Ds

Plasmid

#119077

Purpose

To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loops

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:2xCom-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:sgRNA-MS2-Ds

Plasmid

#119076

Purpose

To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loops

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:2xMS2-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pTY152 EGFP-T2A-ORF6 SARS-CoV-1

Plasmid

#204974

Purpose

Co-express EGFP and SARS-CoV-1 ORF6

Depositor

Tim Mitchison

Article

Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

Open Reading Frame 6 (ORF6 SARS-CoV-1)

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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mIFP12-sEspin-C-18

Plasmid

#56262

Purpose

Localization: Actin Bundling, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

sEspin

Tags

mIFP12

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mCerulean3-EB1-C-18

Plasmid

#55415

Purpose

Localization: MT End Binding Protein, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

EB1 (MAPRE1 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
pEJS436-pCSDest2-AcrIIC2Nme

Plasmid

#85712

Purpose

Mammalian expression of Type II-C anti-CRISPR protein AcrIIC2Nme from Neisseria meningitidis

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

Type II-C anti-CRISPR AcrIIC2Nme

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pEJS481-pCDest2-AcrE2-mTagBFP2-IRES

Plasmid

#85749

Purpose

Expresses anti-CRISPR protein from Type I-E and mTagBFP2

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Inserts

Type I-E anti-CRISPR
mTagBFP2

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEJS443-pCSDest2-AcrIIC3Nme

Plasmid

#85713

Purpose

Mammalian expression of Type II-C anti-CRISPR protein AcrIIC3Nme from Neisseria meningitidis

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

Type II-C anti-CRISPR AcrIIC3Nme

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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mIFP-CytERM-N-17

Plasmid

#56217

Purpose

Localization: Endoplasmic Reticulum, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

CytERM

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP-Paxillin-22

Plasmid

#56234

Purpose

Localization: Focal Adhesions, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Paxillin

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP-MyosinIIC-N-18

Plasmid

#56232

Purpose

Localization: Cytoskeleton, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

MyosinIIC

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mIFP-MannII-N-10

Plasmid

#56229

Purpose

Localization: Mannosidase/Golgi, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

MannII

Tags

mIFP

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pEJS430-pCSDest2-AcrIIC1Boe

Plasmid

#85678

Purpose

Mammalian expression of Type II-C anti-CRISPR protein AcrIIC1Boe from Brackiella oedipodis

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

Type II-C anti-CRISPR AcrIIC1Boe

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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mCerulean3-H2B-C-10

Plasmid

#55422

Purpose

Localization: Nucleus/Histones, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

H2B (H2BC11 Human)

Tags

mCerulean3

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
mCerulean3-Dectin1A-N-10

Plasmid

#55414

Purpose

Localization: Membrane, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Dectin1A (CLEC7A Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Dectin1A-C-10

Plasmid

#55413

Purpose

Localization: Membrane, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Dectin1A (CLEC7A Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
BII-C3H2B

Plasmid

#133387

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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mIFP12-CAF1-C-10

Plasmid

#56246

Purpose

Localization: Chromatin Assembly Factor 1, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

CAF1

Tags

mIFP12

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mIFP24-CAF1-C-10

Plasmid

#56266

Purpose

Localization: Chromatin Assembly Factor 1, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

CAF1

Tags

mIFP24

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-N1-TFE3

Plasmid

#38120

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFE3 (TFE3 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only

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