We narrowed to 13,735 results for: TIM;

1. PT7lacO-His6-AlaRS

   Plasmid

   #224362

   Purpose

   Express AlaRS protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   AlaRS (alaS E. coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFA6a-link-yomCherry-Kan

   Plasmid

   #44903

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mCherry with Kan selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. eMagBF-TgRFPT

   Plasmid

   #162253

   Purpose

   Blue-light dependent heterodimerization system

   Depositor

   Pietro De Camilli

   Article

   Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

   Insert

   eMagBF-TgRFPT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. DL016:CMV::eGFP-iPAK4

   Plasmid

   #177881

   Purpose

   eGFP-IPAK4 fusion with CMV promoter, cloned into a lentivirus backbone

   Depositor

   Adam Cohen

   Article

   Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   iPAK4
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pWRS3001_VCR1_WCR3

   Plasmid

   #192206

   Purpose

   VCR1 and WCR3 tracrRNAs cloned into pTWIST AMP high copy backbone

   Depositor

   William Sellers

   Article

   Li et al Nat Commun. 2022 May 5;13(1):2469. doi:

   Insert

   VCR1-WCR3 tracrRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NeonGreen-C1-ORP3

   Plasmid

   #220070

   Purpose

   overexpresses ORP3 with a neonGreen Fluorophore

   Depositor

   Gerry Hammond

   Article

   Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

   Insert

   OSBPL3 (OSBPL3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. DL017:CMV::HaloTag-iPAK4

   Plasmid

   #177882

   Purpose

   HT-iPAK4 fusion with CMV promoter, cloned into a lentivirus backbone

   Depositor

   Adam Cohen

   Article

   Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   iPAK4
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. NeonGreen-C1-ORP2

   Plasmid

   #220069

   Purpose

   overexpresses ORP2 with a neonGreen fluorophore

   Depositor

   Gerry Hammond

   Article

   Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

   Insert

   OSBPL2 (OSBPL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA5/FRT/TO_NES_HaloTag9

   Plasmid

   #175537

   Purpose

   Strict cytosolic expression of HaloTag9 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   NES-HaloTag9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pnos-Cas9-nos

    Plasmid

    #62208

    Purpose

    Expresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster.

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    cas9
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    nanos

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-HIFR

    Plasmid

    #192946

    Purpose

    Lentiviral production of HIF-1alpha reporter

    Depositor

    Markus Covert

    Article

    Jeknic et al J Biol Chem. 2023 Mar 10:104599. doi: 10

    Insert

    HIF-1alpha reporter
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mCMV

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFastBac_Dual_SNF2L-opt_p48

    Plasmid

    #102243

    Purpose

    Expression of human codon optimized human SNF2L and pRbAp48 from pFastBac dual

    Depositor

    Joe Landry

    Inserts

    SNF2L (SMARCA1 Human)
    pRbAp48 (RBBP4 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    codon optimized for expression

    Promoter

    Polyhedrin and p10

    Available since

    Nov. 1, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. NeonGreen-C1-ORP6

    Plasmid

    #220072

    Purpose

    overexpresses ORP6 with a neonGreen fluorophore

    Depositor

    Gerry Hammond

    Article

    Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

    Insert

    OSBPL6 (OSBPL6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTubulin-Electra2

    Plasmid

    #184930

    Purpose

    Tubulin labeling with blue fluorescent protein Electra2

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    TUBA1B-Electra2
    

    Use

    Tags

    Electra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Myc-PSF-WT

    Plasmid

    #35183

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

    Insert

    PSF (SFPQ Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTubulin-mTagBFP2

    Plasmid

    #184931

    Purpose

    Tubulin labeling with blue fluorescent protein mTagBFP2

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    TUBA1B-mTagBFP2
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHT-ads

    Plasmid

    #47382

    Purpose

    Expresses ads under Pgrac promoter (inducible by IPTG) in bacteria

    Depositor

    Heng-Phon Too

    Article

    Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

    Insert

    ads
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pgrac

    Available since

    Oct. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV_AncBE4max_3xHA

    Plasmid

    #112097

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    AncBE4max_3xHA
    

    Use

    CRISPR

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1(-)-qmTQ2-ATP

    Plasmid

    #225248

    Purpose

    mammalian expression of qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-ATP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(+)-OTI-TCRA_p2_TCRB

    Plasmid

    #131033

    Purpose

    Expresses OT-I TCRA linked to TCRB with the P2A self-cleaving peptide

    Depositor

    Jeff Hammerbacher

    Article

    Aksoy et al bioRxiv

    Inserts

    TCRA (Tcra Mouse)
    TCRB (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/T7

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge