We narrowed to 75,875 results for: Rest
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Plasmid#155220PurposeMonitor changes in the density of membrane-proximal F-actin (MPA)DepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with the CaaX sequence derived from Kras4b
UseTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRV2-GG
Plasmid#105349Purposevirus-induced gene silencingDepositorInsertTRV-RNA2(5')
UseIn planta gene silencing; viralTagsExpressionMutationBsaI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
mc2 mCherry hPGK
Plasmid#206231PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-2
Plasmid#160702Purposegpdh promoter from Trichoderma virens bordered by BsaI restriction sitesDepositorInsertgpdh
UseSynthetic BiologyTagsExpressionMutationIncorporation of BsaI restriction sitesPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
HLA_DRA_01_01
Plasmid#165012PurposeExpression of HLA-DRA*01:01 cDNADepositorInsertHLA-DRA (HLA-DRA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-MPAct-mRuby3-IRES-Puro
Plasmid#155231Purposelentiviral MPAct constructDepositorInsertLentiviral expression of MPAct-mRuby sensor
UseLentiviralTagsmRuby3ExpressionMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
2HA-pDEST-C
Plasmid#118374PurposeGateway compatible mammalian expression vector with C-terminal 2HA to tag proteins of interest.DepositorTypeEmpty backboneUseDestinationTags2HAExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2 Neo
Plasmid#127644PurposeEmpty pLentiCRISPRv2 with NeoRDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBRα-β flap (831-1057)
Plasmid#53734PurposeExpression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorInsertalpha NTD-Beta flap fusion
UseTagsExpressionBacterialMutationPromoterlpp/lacUV5 (tandem promoter)Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
2HA-pDEST-N
Plasmid#118373PurposeGateway compatible mammalian expression vector with N-terminal 2HA to tag proteins of interest.DepositorTypeEmpty backboneUseDestinationTags2HAExpressionMammalianMutationPromoterAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI039
Plasmid#105428PurposeSynthetic biologyDepositorInsertLexA-VP16-ER (transcription factor: inducible expression; estradiol)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
p1D4-hrGFP II
Plasmid#71317Purposeexpression vector with 1D4 epitope added as a C-terminal tag, allowing for in vitro expression of any visual pigment or GPCR of interest.DepositorTypeEmpty backboneUseTags1D4 epitope (TETSQVAPA) and IRES-hrGFPIIExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
mc2 puro
Plasmid#206215PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/p-bacteria
Plasmid#64325PurposeExpresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR; Tetracycline-inducibleTags3xFLAGExpressionBacterialMutationD10A, H840APromoterpLtetO-1Available sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUPD2+p35SoTMV
Plasmid#170876PurposePhytobrick - p35SoTMVDepositorInsertp35SoTMV
UseTagsExpressionPlantMutation214C>T and 229C>T to remove BbsI restrictio…PromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHBV-X/EnhI-Luc
Plasmid#71418PurposeTo see HBV X promoter(overlaped EnhanceI) activity for luciferase assayDepositorInsertHBV X promoter and enhancer I driving Luciferase expression
UseLuciferaseTagsExpressionMammalianMutationPromoterHBV XAvailable sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL4.R_TRE_minPro
Plasmid#211517PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minPro-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterSV40 and minProAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
mc2 mNeon hPGK
Plasmid#206230PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTEI036
Plasmid#105350PurposeSynthetic biologyDepositorInsertLexA operator & 35S min promoter
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-MPAct-mCit-IRES-Neo
Plasmid#155230Purposelentiviral MPAct constructDepositorInsertLentiviral expression of MPAct-mCitrine sensor
UseLentiviralTagsmCitrineExpressionMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only