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pBP_sgRNA

Plasmid

#190128

Purpose

Level 0 MoClo plasmid for Golden Gate cloning of sgRNAs

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pHIS3p:Venus-Tub1+3'UTR::HIS3

Plasmid

#50656

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

Venus-Tub1

Use

Yeast expression

Tags

Venus

Promoter

HIS3p

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-mRuby2

Plasmid

#89686

Purpose

pCAG promoter driving mRuby2

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pET-51b-HaloTag11

Plasmid

#175519

Purpose

Expression of HaloTag11 in bacterial cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag11

Tags

His

Expression

Bacterial

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAN3944

Plasmid

#74702

Purpose

Circuit plasmid: 0x1C

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

Circuit 0x1C

Expression

Bacterial

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pFCK(1.3)GW

Plasmid

#27230

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pAN3945

Plasmid

#74699

Purpose

Circuit plasmid: 0x41

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

Circuit 0x41

Expression

Bacterial

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pHIS3p:mRuby2-Tub1+3'UTR::LEU2

Plasmid

#50645

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

mRuby2-Tub1

Use

Yeast expression

Tags

mRuby2

Promoter

HIS3p

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pQacR-Q2

Plasmid

#74690

Purpose

QacR-Q2 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSQ2-QacR-PQacR-YFP

Expression

Bacterial

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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DsRed-Rab5-QL

Plasmid

#29688

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

RAB5A

Tags

DSRed2

Expression

Mammalian

Mutation

Q79L

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only
pAN4037

Plasmid

#74703

Purpose

Output plasmid: PPhlF-PBetI-YFP

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PPhlF-PBetI-YFP

Expression

Bacterial

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2 DN-GSK3-GFP

Plasmid

#29681

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

glycogen synthase kinase 3

Tags

GFP

Expression

Mammalian

Mutation

K85R and K86R

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
pKDT17

Plasmid

#27503

Depositor

Peter Greenberg

Article

Pearson et al Proc Natl Acad Sci U S A. 1994 Jan 4. 91

Insert

LasR, LasB::LacZ fusion (lasR Pseudomonas aeruginosa)

Expression

Bacterial

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only
pAN4023

Plasmid

#74701

Purpose

Output plasmid: PBM3R1-YFP

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PBM3R1-YFP

Expression

Bacterial

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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p5419

Plasmid

#168186

Purpose

bacteria reporter

Depositor

Michael Hensel

Article

Schulte et al Commun Biol. 2021 May 4;4(1):520. doi: 1

Insert

PBAD::tag-rfp-T PtetA::sfgfp

Expression

Bacterial

Mutation

none

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pQacR-Q1

Plasmid

#74689

Purpose

QacR-Q1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSQ1-QacR-PQacR-YFP

Expression

Bacterial

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pHIS3p:CloverGFP-Tub1+3'UTR::LEU2

Plasmid

#50642

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

CloverGFP-Tub1

Use

Yeast expression

Tags

CloverGFP

Promoter

HIS3p

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

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p5084

Plasmid

#168179

Purpose

bacteria reporter

Depositor

Michael Hensel

Article

Schulte et al Commun Biol. 2021 May 4;4(1):520. doi: 1

Insert

PEM7::dsred PmsrA::sfgfp

Expression

Bacterial

Mutation

none

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pHlyIIR-H1

Plasmid

#74681

Purpose

HlyIIR-H1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSH1-HlyIIR-PHlyIIR-YFP

Expression

Bacterial

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pAmtR-A1

Plasmid

#74676

Purpose

AmtR-A1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSA1-AmtR-PAmtR-YFP

Expression

Bacterial

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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