We narrowed to 2,375 results for: pcas9

1. pJT78_GalL_nCDA1Δ195-BE3

   Plasmid

   #145044

   Purpose

   Expresses nCDA1Δ195-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   nCDA1Δ195-BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   pmCDA1(1-195AA); spCas9(D10A)

   Promoter

   GalL

   Available Since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. LMX1B-gRNA-C-del

   Plasmid

   #131516

   Purpose

   gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-N-del

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   LMX1B-gRNA-C-del
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

3. pGES201

   Plasmid

   #190197

   Purpose

   For CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selection

   Depositor

   Yuefeng Guan

   Article

   Bai et al Plant Biotechnol J. 2020 Mar;18(3):721-7

   Insert

   spCas9
   

   Use

   CRISPR

   Tags

   3xFlag

   Expression

   Plant

   Mutation

   plant-codon optimized

   Promoter

   Glycine max elongation factor 1A(pM4)

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRN3P_T3_ABE8e_IVT

   Plasmid

   #201676

   Purpose

   Plasmid to be used as DNA template for in vitro RNA transcription of the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid contains optimised 5'UTR and 3'UTR to improve protein expression.

   Depositor

   Timo Otonkoski

   Article

   Jalil et al Am J Hum Genet. 2024 Apr 4;111(4):714-72

   Insert

   ecTadA(8e)-nSpCas9
   

   Use

   CRISPR; Vector for in-vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T3 promoter

   Available Since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. iCas

   Plasmid

   #84232

   Purpose

   Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

   Depositor

   Meng How Tan

   Article

   Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

   Insert

   Cas9
   

   Use

   Tags

   2A-OFP co-expression and ERT2-ERT2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDicAID_nCas9-PmCDA_NptII_Della

   Plasmid

   #91694

   Purpose

   Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A for nickase Cas9

   Promoter

   PcUbi

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-CANX

   Plasmid

   #227279

   Purpose

   Expresses SpCas9 and a sgRNA targeting the C-terminus of CANX for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of CANX (CANX Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPBT/EFS-Cas9-PuroTk

   Plasmid

   #170542

   Purpose

   Expresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinase

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Insert

   PuroTK
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-PITCh-TOMM20

   Plasmid

   #207789

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TOMM20 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of TOMM20 (TOMM20 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-PITCh-VIM

    Plasmid

    #227301

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of VIM for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of VIM (VIM Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-PITCh-MAPRE1

    Plasmid

    #207793

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of MAPRE1 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of MAPRE1 (MAPRE1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-CEP135

    Plasmid

    #227286

    Purpose

    Expresses SpCas9 and a sgRNA targeting the N-terminus of CEP135 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of CEP135 (CEP135 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-PITCh-GOLGA2

    Plasmid

    #207791

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of GOLGA2 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of GOLGA2 (GOLGA2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)

    Plasmid

    #199604

    Purpose

    Expression of cell permeable spCas9 in E. Coli.

    Depositor

    Junwei Shi

    Article

    Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

    Insert

    Cas9-T6N
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Hygro-Cas9 donor

    Plasmid

    #86883

    Purpose

    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection

    Depositor

    Danwei Huangfu

    Article

    Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3xFlag, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330A-nBRD4/PITCh

    Plasmid

    #91794

    Purpose

    PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4

    Depositor

    Behnam Nabet, James Bradner

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken β-actin promoter

    Available Since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPBT-PE2-PuroTK-pegRNA_GG

    Plasmid

    #173222

    Purpose

    piggyBac transposon containing both PE2 and a pegRNA cloning site.

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

    Inserts

    PE2
    PuroTK
    pegRNA cloning cassette
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, P2A, and hU6

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCPmacroH2A.1-GS3

    Plasmid

    #204759

    Purpose

    Expression of Cas9 and human macroH2A.1

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    macroH2A1 (MACROH2A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-U6-gRNA-UbC-DsRed-P2A-Bsr

    Plasmid

    #83919

    Purpose

    Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    DsRed-Express2
    Bsr
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A

    Expression

    Mutation

    Promoter

    hUbC

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRubiG-T2A-Cas9

    Plasmid

    #75348

    Purpose

    Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR and Retroviral

    Tags

    GFP (via t2a)

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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