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pMAP L2B

Plasmid

#153357

Purpose

Mobius Assembly cloning and expression vector for plants with high-copy number origin of replication

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Type

Empty backbone

Expression

Plant

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR_TiTRE

Plasmid

#172128

Purpose

Entry vector for tightly controlled gene expression by a tetracycline-responsive promoter

Depositor

Kenji Kontani

Article

Araki et al J Biol Chem. 2021 Nov 18:101428. doi: 10

Type

Empty backbone

Use

Gateway Cloning

Expression

Mammalian

Promoter

tight TRE

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV200

Plasmid

#110719

Purpose

Lentiviral system for reduced ectopic expression.

Depositor

Kevin Dean, Reto Fiolka

Article

Dean et al Biophys J. 2016 Mar 29;110(6):1456-65. d

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pNIC-TolB-NHSTIIT-GG

Plasmid

#215792

Purpose

Cloning of target gene with N terminal TolB SRP pathway signal peptide N terminal His6 Twin Strep tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBXC3H

Plasmid

#47068

Purpose

FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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p7XC3GH

Plasmid

#47066

Purpose

FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Bacterial

Promoter

T7

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pJMP2849

Plasmid

#160677

Purpose

Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI

Plasmid

#44020

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Type

Empty backbone

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCVD433

Plasmid

#63756

Purpose

Derivative of pACYC184 M1uI linkers inserted into EcoRV site inactivating Tc resistance gene. Now Tcs

Depositor

James Kaper

Article

Donnenberg et al Infect Immun. 1990 Jun;58(6):1565-71.

Type

Empty backbone

Expression

Bacterial

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

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pAW091

Plasmid

#113253

Purpose

pUC19 backbone with S. pyogenes CRISPR array

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes CRISPR array

Use

Integration substrate

Promoter

NA

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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