We narrowed to 2,465 results for: Kap

1. pcDNA3.1(+)-Mito-CaNARi

   Plasmid

   #64851

   Purpose

   Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CaNARi
   

   Use

   Tags

   Cerulean, N-terminal 30 amino acids from DAKAP1, …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCS2-mCherry_SNAP_SNAPV_PACT_Dead

   Plasmid

   #173073

   Purpose

   Dead SNAP tag at the centrosome

   Depositor

   Heidi Hehnly

   Article

   Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

   Insert

   mCherry-SNAP-SNAP-Varied-PACT
   

   Use

   Tags

   mCherry-SNAP-SNAP-Varied

   Expression

   Mammalian

   Mutation

   AA144 in both SNAP tags changed from a cysteine t…

   Promoter

   CMV IE94

   Available since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSVE Grb2 myc 49L

   Plasmid

   #26085

   Depositor

   Bob Weinberg

   Article

   Egan et al Nature. 1993 May 6. 363(6424):45-51.

   Insert

   Grb2 P49L (GRB2 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   P49L

   Promoter

   Available since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

4. ch-Tog

   Plasmid

   #28290

   Depositor

   Linda Wordeman

   Article

   Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

   Insert

   ch-TOG (CKAP5 Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   K19R, E118G

   Promoter

   Available since

   April 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDNA3 IKKe T7

   Plasmid

   #27238

   Depositor

   Tom Maniatis

   Article

   Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

   Insert

   I-kappa-B kinase epsilon (IKBKE Human)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pSFG-TFII-I-GFP beta

   Plasmid

   #22200

   Depositor

   Ananda Roy

   Article

   Hakre et al Mol Cell. 2006 Oct 20. 24(2):301-8.

   Insert

   TFII-I (GTF2I Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. eDrTrkA

   Plasmid

   #183996

   Purpose

   Optically controlled eDrTrkA expression in mammalian cells

   Depositor

   Vladislav Verkhusha, Dmitry Gerashchenko

   Article

   Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

   Insert

   eDrTrkA (NTRK1 Human)
   

   Use

   Tags

   Ig kappa secretory signal, mCherry, Deinococcus r…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSFG-TFII-I-GFP delta

   Plasmid

   #22190

   Depositor

   Ananda Roy

   Article

   Desgranges et al Mol Cell Biol. 2005 Dec . 25(24):10940-5

   Insert

   TFII-I (GTF2I Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.4_7C11-1 light chain

   Plasmid

   #198252

   Purpose

   recombinant expression of light chain of monoclonal anti-AMP antibody 7C11-1

   Depositor

   Aymelt Itzen

   Article

   Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

   Insert

   light chain of monoclonal anti-AMP antibody 7C11-1 (mouse kappa)
   

   Use

   Tags

   IL10 signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX Nap4-SH2

    Plasmid

    #46456

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Nap4 (SOCS7 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 395-514

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pZac2.1-GfaABC1D -IgK- TurboID- NCAN IL-HA

    Plasmid

    #219808

    Purpose

    Secreted HA-tagged TurboID-NCAN-IL domain for extracellular proximity labeling

    Depositor

    Cagla Eroglu

    Article

    Irala et al Neuron. 2024 Mar 27:S0896-6273(24)00161-

    Insert

    IgK (Igk Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX-6p-1_GST-DmKHC[1-421]-1xGFP

    Plasmid

    #196973

    Purpose

    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and cleavable N-terminal GST tag

    Depositor

    Lukas Kapitein

    Insert

    Kinesin Heavy Chain (Khc Fly)
    

    Use

    Tags

    GFP and GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX-6p-1_GST-SNAP-DmKHC[1-421]

    Plasmid

    #196976

    Purpose

    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with N-terminal SNAP tag and cleavable N-terminal GST

    Depositor

    Lukas Kapitein

    Insert

    Kinesin Heavy Chain (Khc Fly)
    

    Use

    Tags

    GST and SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. eDrTrkB

    Plasmid

    #183997

    Purpose

    Optically controlled eDrTrkB expression in mammalian cells

    Depositor

    Vladislav Verkhusha, Dmitry Gerashchenko

    Article

    Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

    Insert

    eDrTrkB (NTRK2 Human)
    

    Use

    Tags

    Ig kappa secretory signal, mCherry, Deinococcus r…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET12a-AL-103 Y32F Y96F

    Plasmid

    #47092

    Depositor

    Marina Ramirez-Alvarado

    Article

    DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

    Insert

    AL-103 Y32F Y96F (IGKV3-20 Human)
    

    Use

    Tags

    ompT

    Expression

    Bacterial

    Mutation

    Y32F Y96F

    Promoter

    T7

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pEBG-TFII-I L30&37P

    Plasmid

    #22166

    Depositor

    Ananda Roy

    Article

    Sacristan et al J Biol Chem. 2004 Feb 20. 279(8):7147-58

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Tags

    GST and His

    Expression

    Mammalian

    Mutation

    Changed Leucine 30 to Proline and changed leucine…

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

17. GST-PS-Grb2 (XSB363)

    Plasmid

    #78505

    Purpose

    Construct for expressing Grb2 in E. Coli

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    Grb2 (GRB2 Human)
    

    Use

    Tags

    GST followed by a prescission protease claevage s…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. OMM-V5-LOV-Turbo

    Plasmid

    #199665

    Purpose

    expresses LOV-Turbo on the mammalian outer mitochondrial membrane, lentiviral vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Lentiviral

    Tags

    AKAP1 transmembrane domain and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBMN-HA-TBK1

    Plasmid

    #199205

    Purpose

    Used for the expression of TBK1 (SMC Internal No. 1996)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-scFv-T

    Plasmid

    #67843

    Purpose

    pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.

    Depositor

    Joanna Bereta

    Article

    Ossysek et al Immunol Lett. 2015 Jul 25. pii: S0165-24

    Insert

    antibody 1D8 light kappa chain
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge