We narrowed to 4,418 results for: 1-Oct;

1. pCin4-3xFLAG-MKL1 S454A

   Plasmid

   #19844

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 S454A (MRTFA Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Serine 454 to Alanine

   Available Since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

2. HeFSpCas9

   Plasmid

   #92355

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   Cbh

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MBC1-PuroIII

   Plasmid

   #153419

   Purpose

   MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genes

   Depositor

   Maki Nakayama

   Article

   Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

   Insert

   murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-dSpCas9-HF1-VP64-6xHis

   Plasmid

   #92118

   Purpose

   Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A, N497A, R661A, Q695A, H840A, Q926A

   Promoter

   T7

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEM-HE-Venus-bPAC(F198Y)

   Plasmid

   #165487

   Purpose

   Xenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activity

   Depositor

   Thomas Oertner

   Article

   Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

   Insert

   Venus-bPAC(F198Y)-myc
   

   Use

   Cdna expression, xenopus oocyte

   Tags

   Myc and Venus

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBW2656_pCAG-PV1-nMag-L1-CTD-ENTRY-BGHpA

   Plasmid

   #108849

   Purpose

   Insert protein fragment into KpnI/EcoRI to fuse to nMag tag on N-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   nMag

   Expression

   Mammalian

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pX330-Flag-eSpCas9 (without sgRNA)

   Plasmid

   #92354

   Purpose

   Expression plasmid for human codon-optimized high-fidelity eSpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   3xFLAG-NLS-Streptococcus pyogenes enhanced Cas9-NLS
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   Cbh

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBW2655_pCAG-PV1-NTD-ENTRY-L1-pMag-NLS-BGHpA

   Plasmid

   #108848

   Purpose

   Insert protein fragment into MluI/BspEI to fuse to pMag/NLS tags on C-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   pMag,NLS

   Expression

   Mammalian

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX330-Flag-dHeFSpCas9

   Plasmid

   #92116

   Purpose

   Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive HeFSpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

   Promoter

   Cbh

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBW2653_pCAG-PV1-NTD-ENTRY-L1-nMag-NLS-BGHpA

    Plasmid

    #108846

    Purpose

    Insert protein fragment into MluI/BspEI to fuse to nMag/NLS tags on C-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    nMag,NLS

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBW2067_pCAG-PV1-GAI-L1-CTD-ENTRY-BGHpA

    Plasmid

    #108845

    Purpose

    Insert protein fragment into KpnI/EcoRI to fuse to GAI tag on N-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    GAI

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBW2065_pCAG-PV1-GID1-L1-CTD-ENTRY-BGHpA

    Plasmid

    #108843

    Purpose

    Insert protein fragment into KpnI/EcoRI to fuse to GID1 tag on N-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    GID1

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HA-HIF1a (S668A)

    Plasmid

    #49463

    Purpose

    HA-tagged HIF-1a Ser668 resuide mutated to Ala

    Depositor

    Wafik El-Deiry

    Article

    Warfel et al Cell Cycle. 2013 Oct 25;12(23).

    Insert

    Hypoxia inducible factor 1 alpha (HIF1A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Serine 668 to Alanine

    Promoter

    T7

    Available Since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pET-dHeFSpCas9-VP64-6xHis

    Plasmid

    #92119

    Purpose

    Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive HeFSpCas9-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

    Promoter

    T7

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p3xFLAG-MKL1 D301-380

    Plasmid

    #19852

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 (MRTFA Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    deleted amino acids 301 to 380

    Available Since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pBW1309_pCAG-PV1-FKBP-L1-CTD-ENTRY-BGHpA

    Plasmid

    #108837

    Purpose

    Insert protein fragment into KpnI/EcoRI to fuse to FKBP tag on N-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FKBP

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBW1312_pCAG-PV1-NTD-ENTRY-L1-ABI-NLS-BGHpA

    Plasmid

    #108840

    Purpose

    Insert protein fragment into MluI/BspEI to fuse to ABI/NLS tags on C-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    ABI,NLS

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBW1313_pCAG-PV1-PYL-L1-CTD-ENTRY-BGHpA

    Plasmid

    #108841

    Purpose

    Insert protein fragment into KpnI/EcoRI to fuse to PYL tag on N-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    PYL

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBW2654_pCAG-PV1-pMag-L1-CTD-ENTRY-BGHpA

    Plasmid

    #108847

    Purpose

    Insert protein fragment into KpnI/EcoRI to fuse to pMag tag on N-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    pMag

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBW1308_pCAG-PV1-NTD-ENTRY-L1-FRB-NLS-BGHpA

    Plasmid

    #108836

    Purpose

    Insert protein fragment into MluI/BspEI to fuse to FRB/NLS tags on C-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FRB,NLS

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge