We narrowed to 162,449 results for: addgene

1. pAG415Gal_Gcn4 Swap2

   Plasmid

   #232965

   Purpose

   Galactose iduced expression of Gcn4 k0.19 in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 k0.19
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG415Gal_Gcn4 Swap3

   Plasmid

   #232960

   Purpose

   Galactose iduced expression of Gcn4 k0.47 in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 k0.47
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAG415Gal_Gcn4 W120A

   Plasmid

   #232962

   Purpose

   Galactose iduced expression of Gcn4 W120A in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 W120A
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   W120A

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG415Gal_DBD

   Plasmid

   #232985

   Purpose

   Galactose iduced expression of Gcn4 DBD in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 DBD
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAG415Gal_Gcn4 F124I

   Plasmid

   #232964

   Purpose

   Galactose iduced expression of Gcn4 F124I in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 F124I
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   F124I

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG415Gal_Gcn4 Swap3HA

   Plasmid

   #232998

   Purpose

   Galactose iduced expression of Gcn4 k0.47HA in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 k0.47
   

   Use

   Tags

   3xHA and TEV cleavage site

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAG415Gal_DBDGFP

   Plasmid

   #232999

   Purpose

   Galactose iduced expression of Gcn4 DBDGFP in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   DBD
   

   Use

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSF503

   Plasmid

   #222587

   Purpose

   BB3 gsdA tet-on overexpression cassette with homologous flanks for integration at pyrG locus

   Depositor

   Matthias Steiger

   Article

   Matthias et al PLOS One

   Insert

   gsdA (gsdA )
   

   Use

   A. niger

   Tags

   Expression

   Bacterial

   Mutation

   Internal BsaI and BpiI sites removed from insert,…

   Promoter

   tet-on

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSF8

   Plasmid

   #222592

   Purpose

   BB2 gsdA tet-on overexpression cassette

   Depositor

   Matthias Steiger

   Article

   Matthias et al PLOS One

   Insert

   gsdA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Internal BsaI and BpiI sites removed from insert,…

   Promoter

   tet-on

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTYC10_pPromALB_STARRseq

    Plasmid

    #220289

    Purpose

    STARR-seq plasmid driven by minimal mouse Alb promoter; used to measure enhancer activity of a library of DNA sequences on interest. Useful for STARR-seq analysis in liver.

    Depositor

    David Waxman

    Article

    Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCJ1022

    Plasmid

    #228762

    Purpose

    Plasmid expressing Cas9 and 2 gRNAs for mouse Pkd2. Use for disruption of mouse Pkd2 in cultured cells.

    Depositor

    Bradley Yoder

    Insert

    Pkd2 gRNAs (Pkd2 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. 8V04

    Plasmid

    #220879

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    TMPRSS2 (TMPRSS2 Human)
    

    Use

    Baculovirus expresssion

    Tags

    EFVEHHHHHHHH and Honeybee melittin signal

    Expression

    Mutation

    S251D/R252D/Q253D/S254D/R255K

    Promoter

    Polyhedrin

    Available since

    Sept. 30, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. lentiGuide-dsRed

    Plasmid

    #217006

    Purpose

    lentiviral plasmid for gRNA cloning with selectable dsRed expression

    Depositor

    Deepta Bhattacharya

    Article

    D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. MAC3-N-GFP

    Plasmid

    #222602

    Purpose

    Expression of MAC3-tagged GFP (N-terminal)

    Depositor

    Markku Varjosalo

    Article

    Liu et al Methods Mol Biol. 2023;2690:281-297. doi

    Insert

    EGFP
    

    Use

    Tags

    MAC3

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. MAC3-C-GFP

    Plasmid

    #222601

    Purpose

    Expression of MAC3-tagged GFP (C-terminal)

    Depositor

    Markku Varjosalo

    Article

    Liu et al Methods Mol Biol. 2023;2690:281-297. doi

    Insert

    EGFP
    

    Use

    Tags

    MAC3

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pHR-IDR4-mCh-Cry2WT-NLS

    Plasmid

    #221920

    Purpose

    Expression of CBP IDR4 fragment fused to optogenetic protein mCh-Cry2WT

    Depositor

    Daniel Bose

    Insert

    CBP IDR4 (aa 662-787) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    insertion of the IDR4 region (aa 662-787); insert…

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-CFID-mCh-Cry2WT-NLS

    Plasmid

    #221923

    Purpose

    Expression of CBP CFID fragment fused to optogenetic protein mCh-Cry2WT

    Depositor

    Daniel Bose

    Insert

    CBP CFID (aa 1892-2441) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    insertion of the CBP-FUS interaction domain (CFID…

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHR-IDR3-mCh-Cry2WT-NLS

    Plasmid

    #221919

    Purpose

    Expression of CBP IDR3 fragment fused to optogenetic protein mCh-Cry2WT

    Depositor

    Daniel Bose

    Insert

    CBP IDR3 (aa 441-587) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    insertion of the IDR3 region (aa 441 - 587); inse…

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHR-AL-mCh-Cry2WT-NLS

    Plasmid

    #221924

    Purpose

    Expression of CBP autoregulatory loop (AL) fragment fused to optogenetic protein mCh-Cry2WT

    Depositor

    Daniel Bose

    Insert

    CBP AL (aa 1529-1607) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    insertion of the autoregulatory loop (AL) (aa 152…

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHR-IDR7-mCh-Cry2WT-NLS

    Plasmid

    #221922

    Purpose

    Expression of CBP IDR7 fragment fused to optogenetic protein mCh-Cry2WT

    Depositor

    Daniel Bose

    Insert

    CBP IDR7 (aa 2109-2434) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    insertion of the IDR7 region (aa 2109 - 2434); in…

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge