We narrowed to 2,603 results for: pCas

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Tad-CBE2.4

Plasmid

#235055

Purpose

Expresses Tad-CBE2.4 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE2.4-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE3.6

Plasmid

#235058

Purpose

Expresses Tad-CBE3.6 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE3.6-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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ABE-RA2.1

Plasmid

#208300

Purpose

Expresses ABE-RA2.1 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA2.1-SpCas9 D10A

Expression

Mammalian

Mutation

P48A, R51H, D108G, K110R, H122R, M126I, N127K

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGG184

Plasmid

#165604

Purpose

Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFP

Depositor

Marcus Noyes

Article

Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

Insert

hEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

'CAGCG' PAM replacing the 'CGGCG&#…

Promoter

lac

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9noSsrA

Plasmid

#102285

Purpose

Modified from pCas9-CR4 (Addgene: 62655) to remove the ssrA tag from Cas9.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Mutation

removed ssrA tag

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Drosha_3

Plasmid

#79862

Purpose

CRISPR/Cas9 DROSHA

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

sgRNA Drosha

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pIVT-A3A-DEN2

Plasmid

#238019

Purpose

for DNA-free cytosine base editing in rice and wheat or other plants

Depositor

Caixia Gao

Article

Qiu et al Plant Biotechnol J. 2025 Apr;23(4):1348-

Insert

A3A-nSpCas9(D10A)-UGI

Expression

Plant

Mutation

D10A for SpCas9

Promoter

T7

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pSJX067

Plasmid

#232328

Purpose

Genome editing in Agrobacterium fabrum

Depositor

Wei Zhao

Article

Sun et al Nucleic Acids Res. 2025 Apr 22;53(8):gka

Inserts

sgRNA
SpCas9M

Use

CRISPR

Tags

H-arms and sfGFP

Expression

Bacterial

Promoter

PJ23119 and Pvan

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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HiFi iFE

Plasmid

#190141

Purpose

Expresses HiFiCas9-POLB (HiFi iFE)

Depositor

Timothy Lu

Article

Nakade et al bioRxiv 2022.12.05.518807

Insert

HiFi Cas9-POLB

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

SpCas9 R691A mutant, Codon Optimized for mammalia…

Promoter

CBh

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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px330-RPL26 sgRNA2

Plasmid

#134642

Purpose

contains sgRNA targeting to the C-terminus of human RPL26 for gene editing

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

RPL26 sgRNA2 (RPL26 Human)

Expression

Mammalian

Available Since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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px330-RPL26 sgRNA1

Plasmid

#134641

Purpose

contains sgRNA targeting to the C-terminus of human RPL26 for gene editing

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

RPL26 sgRNA2 (RPL26 Human)

Expression

Mammalian

Available Since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Dgcr8_3

Plasmid

#73527

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

DGCR8

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Dgcr8_4

Plasmid

#73528

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

DGCR8

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Dgcr8_2

Plasmid

#73526

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

DGCR8

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
pFastBac-WT-REPAIR

Plasmid

#252296

Purpose

Expresses REPAIR (WT) in insect cells.

Depositor

Hiroshi Nishimasu

Article

Ishikawa et al Nat Struct Mol Biol. 2025 Aug;32(8):1567

Insert

REPAIR (WT)

Expression

Insect

Mutation

dPspCas13b H133A/H1058A; ADAR2DD E488Q

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pnCas9-miniSdd3-PBE

Plasmid

#204857

Purpose

For cytosine base editing using miniSdd3 in monocotyledon plants (transient transformation)

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd3-nSpCas9(D10A)-UGI

Use

CRISPR

Expression

Plant

Mutation

D10A in SpCas9

Promoter

ZmUbi-1

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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PNLM-pcABE

Plasmid

#237618

Purpose

PNLM-pcABE generated by pre-trained Protein-Nucleic Acid constrained Language Model exhibits high efficiency, a condensed editing window, and a shortened size.

Depositor

Xiaohui Zhang

Article

Ren et al Nat Commun. 2025 Dec 13;16(1):11207. doi

Insert

ecTadA(8e)-nSpCas9

Use

CRISPR

Expression

Mammalian

Mutation

deleted amino acids 2-8 and 147-152

Promoter

pCMV

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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psgRNA-GST-EGFP-GPIDAF

Plasmid

#213719

Purpose

To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF under a CMV promoter.

Depositor

Qilai Huang

Article

Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

Insert

EGFP

Use

CRISPR

Tags

GST

Promoter

CMV

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA2.0

Plasmid

#208299

Purpose

Expresses ABE-RA2.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA2.0-SpCas9 D10A

Expression

Mammalian

Mutation

P48A, R51H, I76F, D108G, K110R, H122R, M126I, N12…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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Yeast_Target-AID3S

Plasmid

#188646

Purpose

Expresses Target-AID3S in yeasts

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Insert

nCas9-AID2S-UGI

Tags

UGI

Expression

Yeast

Mutation

D10A for SpCas9

Promoter

pGal1

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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