We narrowed to 8,554 results for: pCas

1. pBW2698_pCAG-PV1-pMag-L1-iCre-257C-BGHpA

   Plasmid

   #108822

   Purpose

   Expresses aa257 to C-terminus of Cre recombinase fused to pMag CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Cre recombinase fragment aa257 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   NLS and pMag

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBW1606_pCAG-PV1-PYL-L1-PhiC31-397C-BGHpA

   Plasmid

   #108800

   Purpose

   Expresses aa397 to C-terminus of PhiC31 integrase fused to PYL CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment aa397 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   NLS and PYL

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBW1608_pCAG-PV1-PYL-L1-PhiC31-572C-BGHpA

   Plasmid

   #108804

   Purpose

   Expresses aa572 to C-terminus of PhiC31 integrase fused to PYL CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment aa572 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   NLS and PYL

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-2/3-3'

   Plasmid

   #117325

   Purpose

   CRISPR-Cas9 for miR-124-2/3, 3'

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   gRNA miR-124-2/3-3' (MIR124-3 Synthetic)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1-3'

   Plasmid

   #117324

   Purpose

   CRISPR-Cas9 for miR-124-1, 3'

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   gRNA miR-124-1-3' (MIR124-1 Synthetic)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-3-5'

   Plasmid

   #117323

   Purpose

   CRISPR-Cas9 for miR-124-3, 5'

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   gRNA miR-124-3-5' (MIR124-3 Synthetic)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1/2-5'

   Plasmid

   #117322

   Purpose

   CRISPR-Cas9 for miR-124-1/2, 5'

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   gRNA miR-124-1/2-5' (MIR124-1 Synthetic)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCSD_SpCas9MT2-NLS-3xHA-NLS-SaCas9WT-2xNLS

   Plasmid

   #107321

   Purpose

   Expresses SpCas9 (R1333S) fused to SaCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9-SaCas9 fusion
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   SpCas9 (R1333S) is fused to SaCas9

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. BPK4387 - human expression plasmid for SpCas9 Cluster 5

   Plasmid

   #101191

   Purpose

   Human expression plasmid for SpCas9 Cluster 5 variant: CMV-T7-hSpCas9-Cluster5(K918A, V922A, R925A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster5(K918A/V922A/R925A)
   

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   K918A/V922A/R925A

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. MMW3645 - human expression plasmid for SpCas9 Cluster 3

    Plasmid

    #101187

    Purpose

    Human expression plasmid for SpCas9 Cluster 3 variant: CMV-T7-hSpCas9-Cluster3(T657A, G658A, W659A, R661A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster3(T657A/G658A/W659A/R661A)
    

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    T657A/G658A/W659A/R661A

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. MMW3770 - human expression plasmid for SpCas9 Cluster 4

    Plasmid

    #101189

    Purpose

    Human expression plasmid for SpCas9 Cluster 4 variant: CMV-T7-hSpCas9-Cluster4(F491A, M495A, T496A, N497A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster4(F491A/M495A/T496A/N497A)
    

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    F491A/M495A/T496A/N497A

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. MMW2993 - human expression plasmid for SpCas9 Cluster 2

    Plasmid

    #101185

    Purpose

    Human expression plasmid for SpCas9 Cluster 2 variant: CMV-T7-hSpCas9-Cluster2(G582A, V583A, E584A, D585A, N588A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster2(G582A/V583A/E584A/D585A/N588A)
    

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    G582A/V583A/E584A/D585A/N588A

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBW769_pCAG-PV3-FRT-loxP-iRFP720-BGHpA

    Plasmid

    #87576

    Purpose

    PV3 for 2-input, 4-output decoder circuit

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    iRFP720
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBW806_pCAG-PV3-FRT-loxP-GFP-BGHpA

    Plasmid

    #87582

    Purpose

    PV3 for 3-input, 2-output Full Adder

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    EGFP
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCASCADE-fabI-gltA1-gltA2-udhA-zwf

    Plasmid

    #87148

    Purpose

    fabI-gltA1-gltA2-udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Insert

    fabI-gltA1-gltA2-udhA-zwf guide array
    

    Expression

    Bacterial

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCASCADE-fabI-gltA1-gltA2-udhA-gapAP1

    Plasmid

    #87154

    Purpose

    fabI-gltA1-gltA2-udhA-gapAP1 targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Insert

    fabI-gltA1-gltA2-udhA-GapAP1 guide array
    

    Expression

    Bacterial

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCSDest2-SpCas9-MT1-NLS-3XHA-NLS

    Plasmid

    #69221

    Purpose

    Expresses R1333K mutant SpCas9 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Arginine 1333 is changed to Lysine

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCSDest2-SpCas9-MT2-NLS-3XHA-NLS

    Plasmid

    #69222

    Purpose

    Expresses R1333S mutant SpCas9 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Arginine 1333 is changed to Serine

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpCas9(BB)-2A-GFP (PX458)

    Plasmid

    #48138

    Purpose

    Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-TSF-TBK1

    Plasmid

    #216709

    Purpose

    Mammalian expression of TBK1

    Depositor

    James Hurley

    Article

    Tudorica et al J Cell Biol. 2024 Jul 1;223(7):e20230901

    Insert

    TBK1 (TBK1 Human)
    

    Tags

    TSF

    Expression

    Mammalian

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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