We narrowed to 7,250 results for: puc

1. pU3-gRNA

   Plasmid

   #53063

   Purpose

   Transcription of guide RNA (gRNA) in rice cells

   Depositor

   Caixia Gao

   Article

   Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

   Insert

   OsU3 promoter + gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Tags

   Expression

   Mutation

   Promoter

   rice U3

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPRIME-dsRed-shscramble

   Plasmid

   #71384

   Purpose

   Expresses dsRed and scramble shRNA under the control of the CMV promoter in eukaryotic cells

   Depositor

   William Wisden

   Article

   Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

   Insert

   CMV promoter-dsRed-mir30-shscramble
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer/promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMO746

   Plasmid

   #117480

   Purpose

   upp in an artificial operon with npt from pMO9071 and AmpR-pUC ori from pCR4/TOPO, Pnpt-npt-upp;, KanR

   Depositor

   Judy Wall

   Article

   Parks et al Science. 2013 Mar 15;339(6125):1332-5. d

   Insert

   uracil phosphoribosyltransferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAsCpf1(TYCV)(BB) (pY211)

   Plasmid

   #89352

   Purpose

   Expresses humanized AsCpf1 TYCV PAM variant and crRNA guide

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   S542R/K607R

   Promoter

   CBh

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAsCpf1(TATV)(BB) (pY221)

   Plasmid

   #89354

   Purpose

   Expresses humanized AsCpf1 TATV PAM variant and crRNA guide

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1 RVR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   S542R/K548V/N552R

   Promoter

   CBh

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPRIME-dsRed-shvgat

   Plasmid

   #71385

   Purpose

   Expresses dsRed and shRNA against vgat under the control of the CMV promoter in eukaryotic cells

   Depositor

   William Wisden

   Article

   Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

   Insert

   CMV promoter-dsRed-mir30-shvgat
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer/promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAd388-shLuc

   Plasmid

   #83275

   Purpose

   shRNA targeting luciferace

   Depositor

   Joe Landry

   Article

   Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

   Insert

   shRNA to Luciferase
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   pUC ori

   Available since

   Dec. 2, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAAV-CMV-dSa VP64 Hbb

   Plasmid

   #99693

   Purpose

   Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Hbb promoter, vector allows for activation of mouse Hbb, can be packaged and delivered as AAV

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9 and gRNA targeting Hbb (gRNA: GGGGTAAGGGGAGCAAGGTC) (Hbb Mouse, Synthetic)
   

   Use

   AAV and CRISPR

   Tags

   VP64

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. KG#797

   Plasmid

   #110916

   Purpose

   Expresses the active zone - enriched protein Sentryn-GFP in a subset of cholinergic ventral nerve cord motor neurons

   Depositor

   Kenneth Miller

   Article

   Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

   Inserts

   unc-129 promoter
   STRN-1 (Sentryn)-GFP
   unc-54 3' control region with 1 intron just upstream and 1 intron in control region
   

   Use

   Tags

   GFP (contains 3 artificial introns)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. KG#920

    Plasmid

    #110937

    Purpose

    Expresses INS-22-Emerald in the cholinergic motor neuron DA9 for imaging Dense Core Vesicles in a single neuron in a living animal

    Depositor

    Kenneth Miller

    Article

    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

    Inserts

    mig-13 promoter
    INS-22-Emerald
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. KG#493

    Plasmid

    #110932

    Purpose

    Expresses proteins in C. elegans cholinergic neurons in head, ventral nerve cord, and tail

    Depositor

    Kenneth Miller

    Article

    Williams et al Genes Dev. 2007 Nov 1;21(21):2731-46. do

    Inserts

    unc-17 promoter
    unc-54 3' control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. KG#94

    Plasmid

    #110931

    Purpose

    Expresses proteins in C. elegans cholinergic neurons in head, ventral nerve cord, and tail

    Depositor

    Kenneth Miller

    Article

    Williams et al Genes Dev. 2007 Nov 1;21(21):2731-46. do

    Inserts

    unc-17 promoter
    unc-54 3' control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

    Plasmid

    #42335

    Purpose

    A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Has Service

    Cloning Grade DNA

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    Available since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBR_attB(bxb)_lox

    Plasmid

    #183762

    Purpose

    Bxb1-specific donor plasmid for the cloning of payloads <20 kb

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. CLYBL-Bxb1-LP-v2-TC

    Plasmid

    #194327

    Purpose

    CLYBL targeting construct with the Bxb1 landing pad version 2 cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Stem Cell Res. 2023 Feb;66:102991. doi:

    Insert

    loxP-EBFP2-attP-BleoR-lox251
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1-Bxb1-LP-v2-TC

    Plasmid

    #194326

    Purpose

    AAVS1 targeting construct with the Bxb1 landing pad version 2 cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Stem Cell Res. 2023 Feb;66:102991. doi:

    Insert

    loxP-EBFP2-attP-BleoR-lox251
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEFBOS_CreIRESpuro

    Plasmid

    #183812

    Purpose

    Expresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoter

    Depositor

    Ed Stanley

    Article

    Davis et al Nat Protoc. 2008;3(10):1550-8. doi: 10.1

    Insert

    Cre recombinase
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTn7CdrA-gfp(ASV)C

    Plasmid

    #111617

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(ASV)-T0-T1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTn7CdrA-gfpC

    Plasmid

    #111616

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(Mut3)-T0-T1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pU3-gRNA

    Plasmid

    #53063

    Purpose

    Transcription of guide RNA (gRNA) in rice cells

    Depositor

    Caixia Gao

    Article

    Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

    Insert

    OsU3 promoter + gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    Expression

    Mutation

    Promoter

    rice U3

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge