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pBLO 36.02_ProCas9TEV

Plasmid

#121624

Purpose

TetR_tetR/tetA promoters_bCas9-CP199-TEVlinker-2xflag_CM_P15A

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Promoter

p15A

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pXP116

Plasmid

#26813

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pXP120

Plasmid

#26826

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pMAT9

Plasmid

#112590

Purpose

Used for expression of protein in E. coli as Thrombin cleavable N-termina 6His-tag-MBP fusions and without NcoI site within MBP.

Depositor

Marko Hyvönen, Johan Peränen

Article

Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

Type

Empty backbone

Tags

6His-MBP

Expression

Bacterial

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pMST634-BB3_L_AB_hyg_KanOri_pyrG5

Plasmid

#90279

Purpose

BB3 recipient for homologous integration in pyrG locus with linker AB

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pHL 275

Plasmid

#64905

Purpose

KanR + ColE1 + PLlacO-1::RBS(st7):cfp::T1 terminator

Depositor

Han Lim

Article

Hussein et al Biochim Biophys Acta. 2015 May 14;1849(8

Type

Empty backbone

Use

Synthetic Biology; E. coli

Expression

Bacterial

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only
pGGEV_5'_Linker

Plasmid

#49291

Purpose

Golden Gate entry vector at position 5, small linker insert, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pMST636_BB3_L_AD_hyg_KanOri_pyrG5

Plasmid

#90281

Purpose

BB3 recipient for homologous integration in pyrG locus with linker AD

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pKEW270-pTet-empty

Plasmid

#115669

Purpose

Vector for anti-CRISPR expression with pTet

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

pTet

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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CA6

Plasmid

#38992

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

CA6 (CA6 Human)

Tags

Tev His Flag

Expression

Bacterial

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only