We narrowed to 13,217 results for: CRISPR-Cas9

1. AV44_pCAG.Cas9-D10A.gRNA.S1

   Plasmid

   #199258

   Purpose

   Expression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNA

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Inserts

   SpCas9-D10A nickase
   AAVS1-targeting gRNA
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG promoter and RNA polymerase III promoter for …

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. AV15_pCAG.Cas9.gRNA.S1

   Plasmid

   #199259

   Purpose

   Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Inserts

   SpCas9 nuclease
   AAVS1-targeting gRNA
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter and RNA polymerase III promoter for …

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMC-6-AtSpCas9-9

   Plasmid

   #197778

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   AtSpCas9
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAct:dCas9-scFv

   Plasmid

   #78900

   Purpose

   Expresses scFv-VP64 under actin promoter for SunTag CRISPRa in Drosophila cells

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   scFV-VP64
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Insect

   Mutation

   Promoter

   pActin (Drosophila)

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. AV13_pCAG.Cas9.gRNA.NT

   Plasmid

   #199260

   Purpose

   Expression construct encoding SpCas9 nuclease and a control non-targeting guide RNA

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Inserts

   SpCas9 nuclease
   Non-targeting (NT) control gRNA
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter and RNA polymerase III promoter for …

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. EF1a-dCas9-KRAB-GFP backbone

   Plasmid

   #194281

   Purpose

   Vector for CRISPRi-GFP expression ready for gateway cloning of sgRNA

   Depositor

   Elizabeth Kirby

   Article

   Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

   Insert

   dCas9-KRAB-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EFS-CjCas9-eGFP-HIF1a

   Plasmid

   #137929

   Purpose

   Expression of cjCas9 with Hif1a targeting sgRNA

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

   Insert

   CjCas9
   

   Use

   AAV and CRISPR

   Tags

   SV40NLS-HA-T2A-EGFP

   Expression

   Mutation

   Promoter

   EF-1alpha

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PET-HiFi SpCas9-NLS-6xHis

   Plasmid

   #207376

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HiFi SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R691A

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-CFP-SaCas9-HF (without sgRNA)

   Plasmid

   #134470

   Purpose

   Human expression vector for SaCas9-HF variant.

   Depositor

   Zongli Zheng

   Article

   Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p

   Insert

   Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
   

   Use

   CRISPR

   Tags

   SV40NLS-3xFLAG

   Expression

   Mammalian

   Mutation

   R245A, N413A, N419A, R654A

   Promoter

   CAG

   Available since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA

    Plasmid

    #176458

    Purpose

    Vector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT fusion driven by CAG promoter and tagBFP driven by CMV promoter

    Depositor

    Hui Yang

    Article

    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

    Insert

    humanized S. pyogenes Cas9-XTEN-Ec73RT fusion
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-dCas9-MQ1(S317A)-EGFP

    Plasmid

    #89636

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR

    Tags

    3*FLAG and 6*His

    Expression

    Mammalian

    Mutation

    S317A, AGC-->GCC

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. PET-Sniper SpCas9-NLS-6xHis

    Plasmid

    #207374

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    Sniper SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F539S, M763I, K890N

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-dCas9-MQ1(C141S)-EGFP

    Plasmid

    #89635

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR

    Tags

    3*FLAG, 6*His, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    C141S, TGC-->TCC

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-dSpCas9-HF1-VP64-6xHis

    Plasmid

    #92118

    Purpose

    Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, N497A, R661A, Q695A, H840A, Q926A

    Promoter

    T7

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-Flag-eSpCas9 (without sgRNA)

    Plasmid

    #92354

    Purpose

    Expression plasmid for human codon-optimized high-fidelity eSpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFLAG-NLS-Streptococcus pyogenes enhanced Cas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    Cbh

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT3TS-spCas9 ABE-Umax-ex1

    Plasmid

    #222141

    Purpose

    Adenine base editor with modified TadA variant for extended window for A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcs2+spCas9 ABE-Umax-nanos

    Plasmid

    #222142

    Purpose

    Adenine base editor with modified TadA variant for germline A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9 with nanos UTR
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    SP6

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. PET-HypaR-SpCas9-NLS-6xHis

    Plasmid

    #207387

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HypaR-SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R661A, N692A, M694A, Q695A, H698A

    Promoter

    T7

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. PET-SpCas9-HF1-NLS-6xHis

    Plasmid

    #207382

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    T7

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PET-Blackjack SpCas9-NLS-6xHis

    Plasmid

    #207375

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    Blackjack SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    amino acids 1005-1013 replaced with two glycine

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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