We narrowed to 11,340 results for: SHA

1. pG12-SEAP

   Plasmid

   #170271

   Purpose

   Secreted alkaline phosphatase (SEAP) expression in mammalian cells. Reporter plasmid.

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

   Insert

   SEAP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   12xUAS with TATA-box minimal promoter

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mEmerald-Keratin-17

   Plasmid

   #54134

   Purpose

   Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

   Insert

   Keratin (KRT18 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   V282L

   Promoter

   CMV

   Available since

   July 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pQP-iRIS

   Plasmid

   #102584

   Purpose

   Regulation of protein localization (near-infrared-blue-light-inducible shuttle)

   Depositor

   Vladislav Verkhusha

   Article

   Redchuk et al Nat Chem Biol. 2017 Mar 27. doi: 10.1038

   Insert

   BphP1-mVenus-CAAX-IRES2-NES-mCherry-QPAS1-AsLOV2cNLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPAmCherry1-C1

   Plasmid

   #31929

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

   Insert

   PAmCherry1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

   Promoter

   CMV

   Available since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMito-iRFP670

   Plasmid

   #45462

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP670
   

   Use

   Tags

   human cytochrome C oxidase subunit VIII

   Expression

   Mammalian

   Mutation

   T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

   Promoter

   CMV

   Available since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mNG2(1-10)_tdTomato

   Plasmid

   #184169

   Purpose

   Lentiviral vector for expression and selection of the mNG2(1-10) split fluorescent protein.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Inserts

   mNG2(1-10)
   tdTomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTagRFP675-N1

   Plasmid

   #44275

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

   Insert

   TagRFP675
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

   Promoter

   CMV

   Available since

   May 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. piGECInano-N1

   Plasmid

   #186190

   Purpose

   Expression of small FRET-based near-infrared genetically-encoded calcium indicator in mammalian cells

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

   Insert

   iGECInano
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mNeonGreen-mRuby2-FRET-10

   Plasmid

   #58179

   Purpose

   Localization: Cytosolic FRET, Excitation: , Emission:

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2013 May;10(5):407-9. doi:

   Type

   Empty backbone

   Use

   Tags

   mNeonGreen and mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pWS5321-CRISPRai-Markerless

    Plasmid

    #182828

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - markerless

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pscAAV-CBh-aSyn-WPRE3-enSV40pA

    Plasmid

    #194244

    Purpose

    Expresses human alpha-Synuclein WT

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    aSynuclein(WT) (SNCA Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CBh

    Available since

    Jan. 17, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. mOrange2-Lysosomes-20

    Plasmid

    #57968

    Purpose

    Localization: Lysosome Membrane, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    LAMP1
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTcI42-Wasabi

    Plasmid

    #86118

    Purpose

    mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI42

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TcI42
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T, K6N, S33T, Y61H, L119P, F122C

    Promoter

    pR/pL and pR/pL, LacI

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBAD/His-miRFP703

    Plasmid

    #79985

    Purpose

    Monomeric near-infrared fluorescent protein miRFP703 in bacterial plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    miRFP703
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBAD/HisB-PAmKate

    Plasmid

    #32691

    Depositor

    Vladislav Verkhusha

    Article

    Gunewardene et al Biophys J. 2011 Sep 21;101(6):1522-8. Ep

    Insert

    PAmKate
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    F84W/S148N/S165G compared to mKate (but numbering…

    Promoter

    araB

    Available since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTlpA-Wasabi-NF

    Plasmid

    #86100

    Purpose

    Nonfluorescent variant of mWasabi reporter gene gated by wild type TlpA

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D341A and S71T, G73A

    Promoter

    TlpA and TlpA, LacI

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTlpA-Wasabi

    Plasmid

    #85455

    Purpose

    mWasabi reporter gene gated by wild type TlpA

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D341A

    Promoter

    TlpA and TlpA, LacI

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3 HA Sam68 WT

    Plasmid

    #17690

    Depositor

    David Shalloway

    Article

    Lin et al J Biol Chem. 1997 Oct 24. 272(43):27274-

    Insert

    Sam68 (Khdrbs1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWS3799-gRNA-Csy4-template

    Plasmid

    #182827

    Purpose

    SpCas9 gRNA-Csy4 template

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Insert

    SpCas9 gRNA scaffold + Csy4 sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTcI-Wasabi

    Plasmid

    #86101

    Purpose

    mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TcI
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T

    Promoter

    pR/pL and pR/pL, LacI

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge