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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,065 results for: SPL;

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Showing: 2101 - 2120 of 4065 results

  3. NODAL-MYC-DYK-N72A-N199A

    Plasmid
    #115252
    Purpose
    For mammalian expression of the human NODAL open reading frame (with two mutated N-glycosylation sites) with a C-terminal MYC-DYK tag
    Depositor
    Lynne Postovit
    Article
    Findlay et al (2018) bioRxiv 276170
    Insert
    NODAL (NODAL Human)
    Tags
    MYC-DYK
    Expression
    Mammalian
    Mutation
    N72A, N199A
    Promoter
    CMV
    Available Since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    115252-plasmid-map-sequence-id-222234

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  4. NODAL-MYC-DYK-C312A

    Plasmid
    #115253
    Purpose
    For mammalian expression of the human NODAL open reading frame (with a mutated Cysteine residue) with a C-terminal MYC-DYK tag
    Depositor
    Lynne Postovit
    Article
    Findlay et al (2018) bioRxiv 276170
    Insert
    NODAL (NODAL Human)
    Tags
    MYC-DYK
    Expression
    Mammalian
    Mutation
    C312A
    Promoter
    CMV
    Available Since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    115253-plasmid-map-sequence-id-222237

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  8. Rp51a_10bp 5'SS_No BP or pBP_pUC18

    Plasmid
    #100989
    Purpose
    Rp51a with a tGGTAtGTta->AGGTAAGTAT 5'SS mutant with potential to form 10bps with the U1 snRNA. Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation
    Depositor
    Aaron Hoskins
    Article
    Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1
    Insert
    RP51A (RPS17A Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    tGGTAtGTta->AGGTAAGTAT 5'SS mutant, TACTA…
    Available Since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    100989-plasmid-map-sequence-id-194327

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  9. Rp51a_SUS1 5'ss_No_BP_pBP-pUC18

    Plasmid
    #100990
    Purpose
    Contains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence). Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation
    Depositor
    Aaron Hoskins
    Article
    Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1
    Insert
    RP51A (RPS17A Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    GGTAtGT->tGTAtGa 5'SS mutant, TACTAAC->…
    Available Since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    100990-plasmid-map-sequence-id-194330

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  17. pET11a-Z-N-wtBlc

    Plasmid
    #168258
    Purpose
    N-fragment of the wtBlc‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain wtBlc‐split-Zip protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:
    Insert
    N-fragment of wtBlc + Leucine Zipper
    Tags
    His-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168258-plasmid-map-sequence-id-332729

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  18. pMRBad-C-wtBlc

    Plasmid
    #168257
    Purpose
    C-fragment. This plasmid can be co-transformed with either Plasmid 168259: pET11a-N-wtBlc or Plasmid 168472: pET11a-N-DiB2 to obtain wtBlc‐split or DiB2‐split protein, correspondingly
    Depositor
    Jens Meiler
    Article
    Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:
    Insert
    C-fragment of wtBlc
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168257-plasmid-map-sequence-id-332728

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  19. pMRBad-Z-C-wtBlc

    Plasmid
    #168256
    Purpose
    C-fragment. This plasmid can be co-transformed with either Plasmid 168258: pET11a-Z-N-wtBlc or Plasmid 168473: pET11a-Z-N-DiB2 to obtain wtBlc‐split-Zip or DiB2‐split-Zip protein, correspondingly
    Depositor
    Jens Meiler
    Article
    Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:
    Insert
    Leucine Zipper + C-fragment of wtBlc
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168256-plasmid-map-sequence-id-332726

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  20. IBMc120

    Plasmid
    #161950
    Purpose
    Contains elements for building reporter plasmid for IBM. Built upon pSB4A3. ECF20 reporter plasmid
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB4A3-PECF20-B30-mScarlet-I-B15
    Expression
    Bacterial
    Promoter
    PECF20
    Available Since
    March 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    161950-plasmid-map-sequence-id-328229

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Showing: 2101 - 2120 of 4065 results