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pMSCV_IRES_GFP_CD16a

Plasmid

#196189

Purpose

Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

Depositor

Ana Ruiz-Saenz

Publication

Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

Insert

CD16a (FCGR3A Human)

Use

Retroviral

Expression

Mammalian

Mutation

changed Phenylalanine 158 to Valine

Promoter

LTR

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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LentiGuide-TagRFP-2A-BSD

Plasmid

#167930

Purpose

Lentiviral gRNA expression with TagRFP fluorescence and blasticidin selection marker.

Depositor

Ronald Germain

Publication

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1_Floxed-STOP Fluc

Plasmid

#122962

Purpose

Firefly luciferase (Fluc)-based bioluminescent reporter for Cre-loxP DNA recombination reaction

Depositor

Moritoshi Sato

Publication

Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

Insert

pcDNA3.1_Floxed-STOP Fluc

Use

Cre/Lox and Luciferase

Expression

Mammalian

Promoter

CMV

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pTEF2

Plasmid

#43971

Depositor

Hal Alper

Publication

Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Promoter

TEF

Available since

April 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1(+)- e18

Plasmid

#131670

Purpose

Expresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.

Depositor

Alberto Ciccia

Publication

Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

Insert

e18 (engineered RAD18 with SAP domain deleted) (RAD18 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-rtTA

Plasmid

#175274

Purpose

AAV vector mediating neuron-specific expression of rtTA

Depositor

Wei Xu

Publication

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Has service

AAV1

Insert

Reverse tetracycline-controlled transactivator (rtTA)

Use

AAV

Promoter

Synapsin

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pACYC-Skp-TF

Plasmid

#83921

Purpose

Co-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.

Depositor

Karl Griswold

Publication

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
Trigger Factor

Expression

Bacterial

Promoter

T7

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pJW1311

Plasmid

#61254

Purpose

Template for cloning sgRNA(F+E) to generate new PU6::sgRNAs by PCR-fusion

Depositor

Jordan Ward

Publication

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

sgRNA(F+E) targeting Y61A9L9.1

Use

CRISPR; Cloning template

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mRNA-CleanCap-mNeonGreen

Plasmid

#188248

Purpose

Template plasmid to transcribe mRNA encoding mNeonGreen

Depositor

Howard Chang

Publication

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Expression

Mammalian

Promoter

T7

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pZ149-pcDNA3-BhCas12b_v4

Plasmid

#122446

Purpose

Expresses BhCas12b_v4 in mammalian cells

Depositor

Feng Zhang

Publication

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

CMV-NLS-BhCas12b_v4-NLS-3xHA

Expression

Mammalian

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHR_AXP_hrGFP

Plasmid

#84613

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into AXP locus

Depositor

Ian Wheeldon

Publication

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV-TRE-mTurquoise2

Plasmid

#99113

Purpose

An AAV genome with tet-inducible expression of the fluorescent protein mTurquoise2

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mTurquoise2

Use

AAV

Expression

Mammalian

Promoter

TRE

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

Plasmid

#175573

Purpose

Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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4787 TRV2-eTnpBc-HHreRNA4PDS

Plasmid

#251817

Purpose

Editing Photene desaturase (PDS) genes in Nicotiana benthamiana

Depositor

Savithramma Dinesh-Kumar

Publication

Nagalakshmi et al Nat Plants. 2026 Mar;12(3):503-511. doi:

Insert

Enhanced variant of ISDra2 eTnpBc with guide targeting NbPDS flanked by HH-HDV ribozymes

Tags

AtFDnls

Expression

Plant

Mutation

N4Y/R110K/V192L/L222I

Available since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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VV064: 1xCox8 - ratiometric pHluorin in fck

Plasmid

#58502

Purpose

Expresses the pH sensor ratiometric pHluorin in the mitochondria of mammalian cells

Depositor

Adam Cohen

Publication

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - Ratiometric pHluorin

Use

Lentiviral

Expression

Mammalian

Promoter

a-CamKII

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p416 103Q GAL

Plasmid

#1186

Depositor

Susan Lindquist

Publication

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 103Q (HTT Human)

Tags

GFP

Expression

Yeast

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pFH2.10_Gag-MCP-P2A-eGFP

Plasmid

#205525

Purpose

Express HIV Gag-MCP-P2A-eGFP

Depositor

Michael Elowitz

Publication

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Gag-MCP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc

Plasmid

#117728

Purpose

Integration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc gene

Depositor

Andrew Goodman

Publication

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

NanoLuc

Use

Luciferase

Expression

Bacterial

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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HF-PX459 (V2)

Plasmid

#118632

Purpose

Plasmid encoding SpCas9-HF1, a single guide RNA and puromycin resistance

Depositor

Mike McGrew

Publication

Idoko-Akoh et al Sci Rep. 2018 Oct 11;8(1):15126. doi: 10

Insert

hSpCas9-HF1-2A-Puro V2.0

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Asparagine 497 to Alanine, Arginine 661 to Alanin…

Promoter

Cbh

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pRRL-TetO-Pax7-IRES-EGFP

Plasmid

#109372

Purpose

Expresses Dox-inducible Pax7 together with a GFP reporter

Depositor

Nenad Bursac

Publication

Rao et al Nat Commun. 2018 Jan 9;9(1):126. doi: 10

Insert

Pax7

Use

Lentiviral

Expression

Mammalian

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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