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Addgene

We narrowed to 6,719 results for: pcDNA3.1+

Showing: 2101 - 2120 of 6719 results
  1. pCDNA3.1-nAChr-beta3-CFP

    Plasmid
    #50485
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Insert
    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    Use
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    CFP fusion in M3-M4 loop (after residue P379). O…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA3.1(+)_Nav1.6(K1425TAG, Y371C)-HA

    Plasmid
    #239766
    Purpose
    Expresses TTX-resistant (Y371C) variant of mouse Nav1.6 with a TAG codon at position 1425 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.
    Insert
    Scn8a (Scn8a Mouse)
    Use
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    K1425TAG, Y371C
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. pcDNA3.1(+)_Nav1.6(K1546TAG, Y371C)-HA

    Plasmid
    #239767
    Purpose
    Expresses TTX-resistant (Y371C) variant of mouse Nav1.6 with a TAG codon at position 1546 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.
    Insert
    Scn8a (Scn8a Mouse)
    Use
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    K1546TAG, Y371C
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pcDNA3.1-IARS2-H112A/P115A

    Plasmid
    #232338
    Purpose
    Expresses human IARS2 with putative isoleucine binding deficiency mutations (H112A and P115A) in mammalian cells, also carrying synonymous mutations described in Addgene #232340
    Depositor
    Insert
    IARS2 (IARS2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Putative isoleucine binding deficiency mutation H…
    Promoter
    CMV
    Available since
    June 9, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA3.1-IARS2-K664A/K667A

    Plasmid
    #232339
    Purpose
    Expresses human IARS2 with putative tRNA binding deficiency mutations (K664A and K667A) in mammalian cells, also carrying synonymous mutations described in Addgene #232340
    Depositor
    Insert
    IARS2 (IARS2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Putative tRNA binding deficiency mutation K664A A…
    Promoter
    CMV
    Available since
    June 9, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. pcDNA3.1-SARS2-Spike-HiBiT

    Plasmid
    #191212
    Purpose
    Transient expression in mammalian cells of SARS-CoV-2 (A1) spike protein tagged on the C-terminus with HiBiT, the high affinity small fragment of split nanoluciferase assay
    Depositor
    Insert
    SARS-CoV-2 spike_WT (S SARS CoV-2)
    Use
    Tags
    HiBiT (high affinity small nanoluciferase fragmen…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 2101 - 2120 of 6719 results