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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,865 results for: Vars

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Showing: 10661 - 10670 of 11865 results

  4. pVE2_11_p40S_QfDBD_VP16AD_cp_phl_DsRed_0xQUAS

    Plasmid
    #154229
    Purpose
    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_phl (no QUAS control)
    Depositor
    Arnold Driessen
    Article
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    p40S (AN0465 A. nidulans)
    Available Since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154229-plasmid-map-sequence-id-306038

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  5. pNSSCP

    Plasmid
    #183188
    Purpose
    PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under T
    Depositor
    Hsiao-Chun Huang
    Article
    Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1
    Inserts
    DivIVA and superfolder GFP fusion protein
    SpmX450 and T7 181+ fusion protein
    T7 RNAP 1-179 variant and SpmX450 fusion protein
    mRFP1 and popZ fusion protein
    Expression
    Bacterial
    Available Since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183188-plasmid-map-sequence-id-366663

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  6. pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

    Plasmid
    #141236
    Purpose
    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)
    Depositor
    Guohong Cui
    Article
    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do
    Insert
    NES-jRGECO1a
    Use
    AAV and Cre/Lox; Cre-off
    Tags
    6xHIS tag and nuclear export signal
    Promoter
    human elongation factor-1 alpha (EF-1 alpha)
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    141236-plasmid-map-sequence-id-432423

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  8. BPK3258 - human expression plasmid for eSpCas9(1.1)

    Plasmid
    #101176
    Purpose
    Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    K848A/K1003A/R1060A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101176-plasmid-map-sequence-id-193999

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Showing: 10661 - 10670 of 11865 results