Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 15,617 results for: NTS;

Clear keywords
Showing: 1061 - 1070 of 15617 results

  2. pCRISPEY-GAL-Kan-ADE2

    Plasmid
    #232105
    Purpose
    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
    Depositor
    Jeffrey Lewis
    Article
    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:
    Insert
    ADE2 gRNA and repair template
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Repair template introduces C at nt 466 of ADE2 to…
    Promoter
    GAL7
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232105-plasmid-map-sequence-id-462309

    Enlarge

  3. pCRISPEY-GAL-Nat-ADE2

    Plasmid
    #232103
    Purpose
    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
    Depositor
    Jeffrey Lewis
    Article
    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:
    Insert
    ADE2 gRNA and repair template
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Repair template introduces C at nt 466 of ADE2 to…
    Promoter
    GAL7
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232103-plasmid-map-sequence-id-461835

    Enlarge

  4. pU6-Universal.Sth.dCas9

    Plasmid
    #171088
    Purpose
    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    Use
    Toxoplasma gondii expression
    Mutation
    Note: A S245F mutation is present but did not app…
    Promoter
    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171088-plasmid-map-sequence-id-335785

    Enlarge

  9. pChlr-8CQ

    Plasmid
    #52171
    Purpose
    Encodes module 8 with amino acids 12C-16Q for recognition of nt 1A
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12C in module 8
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pChlr-8SE

    Plasmid
    #52172
    Purpose
    Encodes module 8 with amino acids 12S-16E for recognition of nt 1G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16E in module 8
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 1061 - 1070 of 15617 results