We narrowed to 25,030 results for: Nes

1. pAG415-GPDp-EGFP-rap1-K3CR

   Plasmid

   #84534

   Purpose

   This plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeast

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   K(462,463,651)R

   Promoter

   GPD

   Available Since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pAG415-GPDp-EGFP-rap1-K9R

   Plasmid

   #84535

   Purpose

   This plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeast

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   K(43,61,69,117,240,246,527,582,651)R

   Promoter

   GPD

   Available Since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pDONR221-rap1-K462-463-651R

   Plasmid

   #84564

   Purpose

   This plasmid can be used as a donor for subcloning into Gateway destination vectors.

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Gateway donor plasmid

   Tags

   Expression

   Mutation

   K462-463-651R

   Promoter

   none

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221-rap1-K43-117-118R

   Plasmid

   #84565

   Purpose

   This plasmid can be used as a donor for subcloning into Gateway destination vectors.

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Gateway donor plasmid

   Tags

   Expression

   Mutation

   K43-117-118R

   Promoter

   none

   Available Since

   Oct. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.2/V5 hsa-mir-220a

   Plasmid

   #26314

   Depositor

   David Bartel

   Article

   Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

   Insert

   hsa-mir-220a (MIR220A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pBa.GFP-KIF13AS1371D

   Plasmid

   #224417

   Purpose

   Mammalian expression of KIF 13a S1371D

   Depositor

   Marvin Bentley

   Article

   Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

   Insert

   KIF 13a S1371D (Kif13a Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   S1371D

   Promoter

   Chicken Beta actin

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBa.GFP-KIF13AS1371A

   Plasmid

   #224409

   Purpose

   Mammalian expression of KIF 13a S1371A

   Depositor

   Marvin Bentley

   Article

   Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

   Insert

   KIF 13a S1371A (Kif13a Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   S1371A

   Promoter

   Chicken Beta actin

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBa.GFP-KIF13AWLDLE

   Plasmid

   #224410

   Purpose

   Mammalian expression of KIF 13a WLDLE

   Depositor

   Marvin Bentley

   Article

   Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

   Insert

   KIF 13a WLDLE (Kif13a Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   WLDLE

   Promoter

   Chicken Beta actin

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C1-IFT57

   Plasmid

   #218728

   Purpose

   Expresses N-terminally EGFP-tagged IFT57 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Funabashi et al J Cell Biol. 2018 Aug 6;217(8):2867-2876

   Insert

   IFT57 (IFT57 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-UDP1.0-WPRE

    Plasmid

    #220849

    Purpose

    AAV virus packaging plasmid for neuronal expression of an extracellular UDP sensor (UDP1.0)

    Depositor

    Yulong Li

    Article

    Umpierre et al Neuron. 2024 Apr 10:S0896-6273(24)00195-

    Insert

    UDP1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin (hSyn)

    Available Since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLPC-Flag-BirA-Linker-Myc-mRap1

    Plasmid

    #184644

    Purpose

    Retroviral expression of BirA-mRap1

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    BirA-rap1
    

    Use

    Retroviral

    Tags

    BirA FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. puc19_N_tag

    Plasmid

    #186650

    Purpose

    Empty donor plasmid.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    N-terminal 3xFlag-3xHA Tag
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLPC-Flag-BirA-Linker-Myc-mRap1-I312R

    Plasmid

    #184645

    Purpose

    Retroviral expression of BirA Rap1 I312R

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    BirA-rap1-I312R
    

    Use

    Retroviral

    Tags

    BirA FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJCC_164 SpCas9 R1333A/R1335A

    Plasmid

    #179528

    Purpose

    For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 R1333A/R1335A
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed arginines 1333 and 1335 to alanine

    Promoter

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMP71-mGFP-llo190-mc38minor

    Plasmid

    #174604

    Purpose

    expresses palmitoylated GFP with a C terminal fusion of the LLO190 and minigenes of 3 neoantigens from the MC38 tumor in genes Aatf, irgq and cpne1

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    palmitoyl-GFP C-term fusion LLO190 and minigenes of 3 neoantigens from MC38 tumor in genes Aatf irgq cpne1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-3)

    Plasmid

    #159664

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-2)

    Plasmid

    #159663

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-1)

    Plasmid

    #159662

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-1)

    Plasmid

    #159656

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-dt/deltaEGFP

    Plasmid

    #163919

    Purpose

    PGK-driven dTomato expression. Lacks the CBA-promoter controlling EGFP expression.

    Depositor

    John Engelhardt

    Article

    Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

    Insert

    dtomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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