We narrowed to 8,881 results for: lic

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pGex-4T-2_GEC1

Plasmid

#73945

Purpose

For recombinant expression of human GEC1/GABARAPL1 in E. coli

Depositor

Dieter Willbold

Publication

Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

Insert

GEC1 (GABARAPL1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pMpGE_En01

Plasmid

#71534

Purpose

Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

Depositor

Ikuko Hara-Nishimura, Takayuki Kohchi

Publication

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Available since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV_Cas9–superNLS

Plasmid

#232430

Purpose

Expression plasmid for the SpCas9 nuclease with superNLS

Depositor

Gil Westmeyer

Publication

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

Cas9–superNLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pMAH2-CageCys

Plasmid

#71404

Purpose

Expresses tRNA and synthetase for mammalian encoding of a photocaged cysteine

Depositor

Huiwang Ai

Publication

Ren et al J Am Chem Soc. 2015 Feb 18;137(6):2155-8

Insert

aminoacyl-tRNA synthetase for a caged cysteine

Promoter

CMV

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pET28a-SARS-CoV-2 NSP13

Plasmid

#176492

Purpose

Expresses the N-terminal His6-tagged SARS-CoV2 helicase (NSP13) in E coli.

Depositor

Xiaodong Cheng

Publication

White et al J Phys Chem Lett. 2020 Nov 5;11(21):9144

Insert

SARS-CoV-2 NSP13 (ORF1ab SARS-CoV)

Tags

His6 and T7

Expression

Bacterial

Mutation

I570 from SARS-CoV NSP13 was mutated to V to conv…

Promoter

T7

Available since

Jan. 10, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pAM4787

Plasmid

#120088

Purpose

Cyanobacteria synthetic plasmid to test the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS).

Depositor

Susan Golden

Publication

Chen et al Microbiology. 2016 Dec;162(12):2029-2041

Insert

Plasmid assembled from 4 CYANO-VECTOR modular devices: CV-pANS, CV-oribom, CV-aadA, PconII-LTRBS-yemGFP.

Use

Other

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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AAT-PB-CG2APtk

Plasmid

#86003

Purpose

homology donor for AAT locus with piggyBac-flanked eGFP_T2A_puroTK cassette

Depositor

Tobias Cantz

Publication

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

eGFP_T2A_puro_dTK

Use

Piggybac exciseable

Expression

Mammalian

Promoter

CAG

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Delta DED FLIP-L D/A

Plasmid

#13791

Depositor

Guy Salvesen

Publication

Boatright et al Biochem J. 2004 Sep 1. 382(Pt 2):651-7.

Insert

DELTA DED FLIP LONG (CFLAR Human)

Tags

8 x His tag

Expression

Bacterial

Mutation

Delta DED removes first 186 residues Asp to Ala m…

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only

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pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX)

Plasmid

#86424

Purpose

PCR tagging module with yeast codon optimized mTurquoise2

Depositor

Sue Jaspersen

Publication

Burns et al Elife. 2015 Sep 15;4. doi: 10.7554/eLife

Insert

mTurquoise2

Expression

Yeast

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-H2B-6

Plasmid

#55056

Purpose

Localization: Nucleus/Histones, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Publication

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

H2B (H2BC11 Human)

Tags

mCherry

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
MSCV N-term HA-FLAG puro BRD7

Plasmid

#41934

Depositor

Stephen Elledge

Publication

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Tags

HA-Flag

Expression

Mammalian

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
pLG028 (hhe)

Plasmid

#157906

Purpose

Expresses the hhe defense system

Depositor

Feng Zhang

Publication

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

hhe (DUF4011-helicase-Vsr-DUF3320)

Expression

Bacterial

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSb_init containing the MBP sybody Sb_MBP#1

Plasmid

#132699

Purpose

expresses the MBP sybody Sb_MBP#1 in E.coli, to use as positive control in ELISA

Depositor

Markus Seeger

Publication

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

sybody_MBP#1

Expression

Bacterial

Promoter

pBAD

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAM1414

Plasmid

#40237

Depositor

Susan Golden

Publication

Andersson et al Methods Enzymol. 2000;305:527-42.

Insert

promoterless luxAB reporter

Use

Cyanobacteria cloning vector

Promoter

n/a

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAR4-NLS

Plasmid

#138217

Purpose

Nuclear-targeted FRET-based PKA activity reporter.

Depositor

Jin Zhang

Publication

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

AKAR4-NLS

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-21a_2xNLS_LbCpf1_protein_expression

Plasmid

#114366

Purpose

Protein expression plasmid for 2xNLS LbCpf1 in pET-21a backbone

Depositor

Scot Wolfe

Publication

Xu et al Blood. 2019 Jan 31. pii: blood-2019-01-8

Insert

2xNLS_LbCpf1

Use

CRISPR

Tags

NLS

Expression

Bacterial

Promoter

T7

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCMV-Rev(NL4.3)

Plasmid

#115776

Purpose

pCMV-RevNL4.3 allows the expression of Rev protein.

Depositor

Damian Purcell

Publication

Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

Insert

HIV-1 Rev protein (NL4.3)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Tus pGST-//

Plasmid

#53305

Purpose

Expresses GST-tagged E.coli replication terminator protein Tus.

Depositor

John Lis

Publication

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Insert

E.Coli Tus replication terminator protein

Tags

GST

Expression

Bacterial

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLSLGFP

Plasmid

#51494

Purpose

pLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid

Depositor

Daniel Voytas

Publication

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

GFP
us:NPTII repair template

Use

Plant t-dna plasmid

Tags

NLS and flag

Promoter

virion-sense LIR and 2x35S

Available since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBBR_Cas9_sp1

Plasmid

#154851

Purpose

Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome

Depositor

John van der Oost

Publication

Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.

Inserts

Spcas9 gene
sgRNA scaffold

Use

CRISPR

Tags

6xHis

Mutation

Codon harmonized variant for expression in Rhodob…

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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