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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,092 results for: pcdna3.1

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Showing: 2121 - 2140 of 7092 results

  2. pcDNA3.1-HaloCKAR2.2-T2A-EGFP

    Plasmid
    #216493
    Purpose
    Expression of chemigenetic PKC biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells
    Depositor
    Jin Zhang
    Publication
    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HaloCKAR2.2
    Tags
    T2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    216493-plasmid-map-sequence-id-431037

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  3. pcDNA3.1-HaloEKAR2.2-T2A-EGFP

    Plasmid
    #216495
    Purpose
    Expression of chemigenetic ERK biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells
    Depositor
    Jin Zhang
    Publication
    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HaloEKAR2.2
    Tags
    T2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    216495-plasmid-map-sequence-id-431035

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  4. pcDNA3.1-HaloAKAR2.1-T2A-EGFP

    Plasmid
    #216489
    Purpose
    Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and EGFP transfection marker in mammalian cells
    Depositor
    Jin Zhang
    Publication
    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HaloAKAR2.1
    Tags
    T2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    216489-plasmid-map-sequence-id-431030

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  5. pcDNA3.1-FLT3-D835Y-V5/HIS

    Plasmid
    #236007
    Purpose
    expression of the D835Y kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia and Acute Lymphoblastic Leukemia
    Depositor
    Pavel Krejčí
    Publication
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human FLT3-D835Y receptor tyrosine kinase, full length (FLT3 Human)
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    D835Y substitution
    Promoter
    CMV
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    236007-plasmid-map-sequence-id-469607

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  8. pcDNA3.1-MS2-HA-Hs4E-T_AA

    Plasmid
    #148233
    Purpose
    Mammalian Expression of Hs4E-T
    Depositor
    Elisa Izaurralde
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Hs4E-T (EIF4ENIF1 Human)
    Expression
    Mammalian
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148233-plasmid-map-sequence-id-473836

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  10. pcDNA3.1-RIαΔ(D/D+linker)-EGFP

    Plasmid
    #181843
    Purpose
    GFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.
    Depositor
    Jin Zhang
    Publication
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RIα-(D/D+linker)EGFP (PRKAR1A Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Deletion of residues 12-112 in PKA RIα.
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    181843-plasmid-map-sequence-id-364312

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  11. pcDNA3.1(+)-cpPKN-mRuby2-GFP1-10

    Plasmid
    #181859
    Purpose
    Part of FluoSTEP-RhoA biosensor; must be expressed with GFP11-tagged RhoA plasmid (Addgene #181860) or in GFP11-RhoA gene-edited cells.
    Depositor
    Jin Zhang
    Publication
    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cp-PKN-mRuby2-GFP(1-0)
    Tags
    mRuby2 and sfGFP(1-10)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    181859-plasmid-map-sequence-id-363186

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  12. pcDNA3.1(+)-mCherry-p115(S240A)

    Plasmid
    #181997
    Purpose
    Mammalian expression vector encoding S240A-mutated p115 RhoGEF tagged with mCherry.
    Depositor
    Jin Zhang
    Publication
    Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-p115(S240A) (ARHGEF1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Serine 240 changed to alanine in p115.
    Promoter
    CMV
    Available since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181997-plasmid-map-sequence-id-362770

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  20. pcDNA3.1-mCherry-CyrCR1.mutant

    Plasmid
    #128746
    Purpose
    Expresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.
    Depositor
    David Bartel
    Publication
    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)
    Expression
    Mammalian
    Mutation
    5 point mutations disrupting the miR-7 site
    Promoter
    CMV
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128746-plasmid-map-sequence-id-254125

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Showing: 2121 - 2140 of 7092 results