We narrowed to 5,308 results for: pcdna3.1

1. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S KK93EE

   Plasmid

   #166796

   Purpose

   Mammalian cell expression vector for FLAG-tagged DPP9S. KK93EE mutant is partial loss of CARD8 binding.

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Use

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Mutation

   KK93EE

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S E597R

   Plasmid

   #166800

   Purpose

   Mammalian cell expression vector for FLAG-tagged DPP9S. E597R mutant is partial loss of CARD8 binding.

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Use

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Mutation

   E597R

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1 Flag YFP hNKCC1 HA-ECL3 (NT933)

   Plasmid

   #49064

   Purpose

   Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #3 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Use

   Tags

   3x Flag and mVenus

   Expression

   Mammalian

   Mutation

   2xHA epitope in ECL3 inserted at aa460 in hNKCC1 …

   Promoter

   CMV

   Available since

   Nov. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p5064 pcDNA3.1/V5-His-TOPO hBrd4 (1-461)

   Plasmid

   #14443

   Depositor

   Peter Howley

   Article

   You et al Cell. 2004 Apr 30. 117(3):349-60.

   Insert

   Brd4 (1-461) (BRD4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   amino acids 1-461

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pCDNA3.1(-) + SLNCR1 _462-572 (conserved sequence)

   Plasmid

   #86818

   Purpose

   mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

   Depositor

   Carl Novina

   Article

   Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

   Insert

   LINC00673 (LINC00673 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1(+) Laccase2 MCS-ZKSCAN1 548-1047

   Plasmid

   #69896

   Purpose

   Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1 (stw, ZKSCAN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1 Flag YFP hNKCC1 A734C (NT508)

   Plasmid

   #50888

   Purpose

   Expresses human NKCC1 A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   A734C in hNKCC1 in NT17

   Promoter

   CMV

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. NC12 pCDNA3.1 VENUS WTX delta ETGE

   Plasmid

   #36967

   Depositor

   Randall Moon

   Article

   Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

   Insert

   WTX (delta ETGE motif) (AMER1 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   lacks amino acids 288 –291

   Promoter

   CMV

   Available since

   July 24, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1(+)_Nav1.6(K1425TAG, Y371C)-P2A-eGFP

   Plasmid

   #239769

   Purpose

   Expresses independantly TTX-resistant (Y371C) variant of mouse Nav1.6 with a TAG codon at position 1425 and eGFP in mammalian cells.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

   Insert

   Scn8a (Scn8a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K1435TAG, Y371C

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FLAG-GRP78-No UTRs AAA Mutant (pcDNA3.1)

    Plasmid

    #238430

    Purpose

    Expresses GRP78 AAA mutant without the UTRs fused to FLAG tag

    Depositor

    Amy Shiu Lee

    Article

    Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

    Insert

    78-kDa glucose-regulated protein (HSPA5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    The UTRs of the gene have been deleted. The PPP t…

    Promoter

    Available since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FLAG-GRP78-No UTRs Wild-Type (pcDNA3.1)

    Plasmid

    #238429

    Purpose

    Expresses GRP78 Full Length without UTRs fused to the FLAG Tag

    Depositor

    Amy Shiu Lee

    Article

    Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

    Insert

    78-kDa glucose-regulated protein (HSPA5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    The UTRs of the gene have been deleted.

    Promoter

    Available since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-L928A-M929A

    Plasmid

    #233065

    Purpose

    Expresses tethered-agonist dead mouse ADGRL3-L928A-M929A (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.

    Depositor

    Jonathan Javitch

    Article

    Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

    Insert

    ADGRL3 (Adgrl3 Mouse)
    

    Use

    Tags

    ALFA and HiBiT

    Expression

    Mammalian

    Mutation

    Leucine 928 to Alanine; Methionine 929 to Alanine

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-SLC25A46[G249D]-P2A-Electra1 (pAH10)

    Plasmid

    #227615

    Purpose

    Overexpression of SLC25A46 with G249D mutation in mammalian cell lines

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Use

    Tags

    P2A-mElectra1

    Expression

    Mammalian

    Mutation

    G249D

    Promoter

    CMV, T7

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-HA-GTSE1-S91, 262 ,331, 724A

    Plasmid

    #224738

    Purpose

    For expression of HA-tagged GTSE1 protein with S91, 262 ,331, 724A mutation

    Depositor

    Michele Pagano

    Article

    García-Vázquez et al bioRxiv 2024.06.26.600797

    Insert

    GTSE1 (GTSE1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Serine 91, 262 ,331, 724 to Alanine

    Promoter

    CMV

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA

    Plasmid

    #228530

    Purpose

    Firefly luciferase in fusion with a fragment of human Skp1 protein through a flexible linker and a TEV viral protease cleavage site. Expression of fLuc protein is deinhibited by TEV site cleavage.

    Depositor

    Roman Jerala

    Article

    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

    Insert

    myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA (SKP1 Human, Photinus pyralis, Synthetic)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-SLC25A46[R257Q]-P2A-Electra1 (pAH11)

    Plasmid

    #227616

    Purpose

    Overexpression of SLC25A46 with R257Q mutation in mammalian cell lines

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Use

    Tags

    P2A-mElectra1

    Expression

    Mammalian

    Mutation

    R257Q

    Promoter

    CMV, T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-SLC25A46[R340C]-P2A-Electra1 (pAH12)

    Plasmid

    #227617

    Purpose

    Overexpression of SLC25A46 with R340C mutation in mammalian cell lines

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Use

    Tags

    P2A-mElectra1

    Expression

    Mammalian

    Mutation

    R340C

    Promoter

    CMV, T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-SLC25A46[I349D]-P2A-Electra1 (pAH13)

    Plasmid

    #227618

    Purpose

    Overexpression of SLC25A46 with I349D mutation in mammalian cell lines

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Use

    Tags

    P2A-mElectra1

    Expression

    Mammalian

    Mutation

    I349D

    Promoter

    CMV, T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. His-Precission-NEDD4L (381-975)_pcDNA3.1(+)

    Plasmid

    #187725

    Purpose

    Express his-tagged NEDD4L W2-W3-W4-HECT (381) in mammalian cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 381-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Mammalian

    Mutation

    deletion of residues 1-380

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. His-Precission-NEDD4L (492-975)_pcDNA3.1(+)

    Plasmid

    #187726

    Purpose

    Express his-tagged NEDD4L W3-W4-HECT in mammalian cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 492-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Mammalian

    Mutation

    deletion of residues 1-491

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge