We narrowed to 5,560 results for: pcas

1. pCAG-GPHN-FingR-mVenus-CCR5TC

   Plasmid

   #231783

   Purpose

   Visualization of inhibitory post-synapse using GPHN-FingR-mVenus

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   GPHN-FingR-mVenus-CCR5TC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Actin promoter with CMV enhancer

   Available since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-GPHN-FingR-mGold2t-CCR5TC

   Plasmid

   #231786

   Purpose

   Visualization of inhibitory post-synapse using GPHN-FingR-mGold2t

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   GPHN-FingR-mGold2t-CCR5TC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Actin promoter with CMV enhancer

   Available since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGEN-Dre-nls (JDW 1131)

   Plasmid

   #229809

   Purpose

   A CAGGS-driven dre recombinase followed by a c-terminal 1x nls sequence

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   DRE recombinase-nls
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS puro human RLIM N581K

   Plasmid

   #232242

   Purpose

   Mammalian expression of Human RLIM N581K

   Depositor

   Francisco Bustos

   Article

   Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

   Insert

   Human RLIM N581K (RLIM Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N581K

   Promoter

   Available since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-Twin-strep-TEV-DGKδ2

   Plasmid

   #223720

   Purpose

   Express human diacylglycerol kinase delta 2 protein (N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

   Depositor

   Chiaki Murakami

   Insert

   DGKD (DGKD Human)
   

   Use

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted start codon

   Promoter

   CAG and chicken β-actin promoter

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-PHOSPHO2-TEV-Twin-Strep

   Plasmid

   #222016

   Purpose

   Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

   Depositor

   Chiaki Murakami

   Insert

   PHOSPHO2 (PHOSPHO2 Human)
   

   Use

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted stop codon (TAA)

   Promoter

   chicken β-actin promoter

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-PHOSPHO1-TEV-Twin-Strep

   Plasmid

   #222015

   Purpose

   Express human PHOSPHO1 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

   Depositor

   Chiaki Murakami

   Insert

   PHOSPHO1 (PHOSPHO1, PHOSPHO1 Human)
   

   Use

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   stop codon (TGA) is deleted

   Promoter

   chicken β-actin promoter

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-U6-BRD4-HaloTag-Guide-hspCas9

   Plasmid

   #228576

   Purpose

   Expression of Halo-tagged human BRD4 gRNA; CRISPR-mediated gene insertion

   Depositor

   Marcin Imielinski

   Insert

   BRD4 gRNA (BRD4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

   Plasmid

   #223323

   Purpose

   Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).

   Depositor

   Jonathan Arias

   Article

   Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

   Insert

   SpCas9 and sgRNA-T2 (cas9 )
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCA528 IST1 L328D, L355A (1-366)

    Plasmid

    #193040

    Purpose

    Bacterial expression plasmid for full-length IST1 (1-366) with L328D and L355A mutations that diminish binding to MIT domains.

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    L328D, L355A

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG CAPN7 V18D,F98D-OSF

    Plasmid

    #193045

    Purpose

    Mammalian expression plasmid encoding full-length CAPN7 with V18D and F98D mutations that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    OSF (One-Strep-FLAG)

    Expression

    Mammalian

    Mutation

    V18D, F98D

    Promoter

    Chicken Beta-actin

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCA528 CAPN7 V18D,F98D (1-165)

    Plasmid

    #193034

    Purpose

    Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with V18D and F98D mutations that abolish binding to IST1.

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    V18D, F98D, Residues 1-165

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT3TS-spCas9 ABE-Umax-ex1

    Plasmid

    #222141

    Purpose

    Adenine base editor with modified TadA variant for extended window for A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcs2+spCas9 ABE-Umax-nanos

    Plasmid

    #222142

    Purpose

    Adenine base editor with modified TadA variant for germline A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9 with nanos UTR
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    SP6

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

    Plasmid

    #221552

    Purpose

    Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human Rab7A (RAB7A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJS-BCD-Strep-PCaspase-R153A

    Plasmid

    #214036

    Purpose

    Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) R153A mutant from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    PCaspase
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    R153A

    Promoter

    T7 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS-3xFLAG-bIV spectrin (repeats 13-15)

    Plasmid

    #213728

    Purpose

    Mammalian cell expression vector for overexpression of bIV spectrin (repeats 13-15) in NIH/3T3 cells.

    Depositor

    Chaolin Zhang

    Article

    Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

    Insert

    bIV spectrin (repeats 13-15) (Sptbn4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-3xFLAG-bII spectrin (repeats 13-15)

    Plasmid

    #213729

    Purpose

    Mammalian cell expression vector for overexpression of bII spectrin (repeats 13-15) in NIH/3T3 cells.

    Depositor

    Chaolin Zhang

    Article

    Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

    Insert

    bII spectrin (repeats 13-15) (Spnb3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAGG-GST-TEV-mCherry-NAP1 (WT)

    Plasmid

    #198036

    Purpose

    Used for the expression and purification of mCherry NAP1 in a mammalian expression system (SMC Internal No. 2065)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NAP1 (AZI2 Human)
    

    Use

    Tags

    GST-TEV and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCaUra3-CUbo(K48R/K63R)-yeGFP

    Plasmid

    #212774

    Purpose

    C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP in budding yeast with CaUra3 selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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