We narrowed to 13,224 results for: sgrna

1. lentiCRISPRv2_ZsG gRNA_1

   Plasmid

   #192685

   Purpose

   Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

   Depositor

   Jeffrey Miner

   Insert

   Cas9, ZsGreen targeting sgRNA #1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a, U6

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPRv2_ZsG gRNA_2

   Plasmid

   #192686

   Purpose

   Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

   Depositor

   Jeffrey Miner

   Insert

   Cas9, ZsGreen targeting sgRNA #2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a, U6

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCRISPRv2_ZsG gRNA_3

   Plasmid

   #192687

   Purpose

   Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

   Depositor

   Jeffrey Miner

   Insert

   Cas9, ZsGreen targeting sgRNA #3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a, U6

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. SaCas9 GFP V3

   Plasmid

   #226962

   Purpose

   CBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJR98

   Plasmid

   #187239

   Purpose

   CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJMP3

   Plasmid

   #79875

   Purpose

   Bacillus subtilis sgRNA expression vector; integrates into thrC

   Depositor

   Carol Gross, Stanley Qi, Jason Peters

   Article

   Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

   Insert

   sgRNA RR1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   veg

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX458 V3

   Plasmid

   #226957

   Purpose

   CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Pdg459-eSp(1.1) V3

   Plasmid

   #226965

   Purpose

   CBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PX458 eSp(1.1) V3

   Plasmid

   #226963

   Purpose

   CBh-eSpCas9(1.1)-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJMP2

    Plasmid

    #79874

    Purpose

    Bacillus subtilis sgRNA expression vector; integrates into amyE

    Depositor

    Carol Gross, Stanley Qi, Jason Peters

    Article

    Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

    Insert

    sgRNA RR1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    veg

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV_ACTB CRISPIE

    Plasmid

    #172853

    Purpose

    Actb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Inserts

    Actb intron 1 sgRNA
    DRS-2 sgRNA
    CRISPIE designer exon (phase 0-0) encoding mEGFP
    mRuby3
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFD5_w

    Plasmid

    #112645

    Purpose

    Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.

    Depositor

    Michael Boutros

    Insert

    U6:3-tRNA-sgRNA expression cassette
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWS082

    Plasmid

    #90516

    Purpose

    sgRNA entry vector

    Depositor

    Tom Ellis

    Insert

    Yeast sgRNA expression vector
    

    Use

    CRISPR; Yeast gap repair

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBHM2644

    Plasmid

    #229528

    Purpose

    Carries AID* tag, empty sgRNA, and NATR marker

    Depositor

    Hiten Madhani

    Insert

    AID* - empty sgRNA - NATR
    

    Use

    Tags

    2xFLAG

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBHM2645

    Plasmid

    #229529

    Purpose

    Carries mIAA7_opt tag, empty sgRNA, and NATR marker

    Depositor

    Hiten Madhani

    Insert

    mIAA7_opt - empty sgRNA - NATR
    

    Use

    CRISPR

    Tags

    2xFLAG

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBHM2650

    Plasmid

    #229992

    Purpose

    Carries AID* tag, empty sgRNA, and HYGR marker

    Depositor

    Hiten Madhani

    Insert

    AID* - empty sgRNA - HYGR
    

    Use

    Tags

    2xFLAG

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBHM2652

    Plasmid

    #229994

    Purpose

    Carries AID* tag, empty sgRNA, and amdS marker

    Depositor

    Hiten Madhani

    Insert

    AID* - empty sgRNA - amdS
    

    Use

    Tags

    2xFLAG

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDECKO_GFP_AAVS1_186

    Plasmid

    #159091

    Purpose

    targeting, non-essential locus control

    Depositor

    Rory Johnson

    Insert

    gRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDECKO_mCherry_AAVS1

    Plasmid

    #141212

    Purpose

    pDECKO_mCherry_AAVS1_186. Targeting, non-essential locus control

    Depositor

    Rory Johnson

    Insert

    gRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. gRNA telo/GFP

    Plasmid

    #137713

    Purpose

    Vector co-expressing sgRNA telo and GFP

    Depositor

    Dominik Niopek

    Insert

    sgRNA telo + GFP
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6/RSV

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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