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We narrowed to 6,478 results for: nls

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Showing: 2161 - 2180 of 6478 results

  2. pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139087
    Purpose
    Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    SpCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139087-plasmid-map-sequence-id-273883

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  3. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139089
    Purpose
    Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    xCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139089-plasmid-map-sequence-id-273885

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  5. pCAG-hAsCas12a-enRR(E174R/S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1077)

    Plasmid
    #114095
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a-enRR(E174R/S542R/K607R) nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-RR (E174R/S542R/K607R)
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    E174R, S542R and K607R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114095-plasmid-map-sequence-id-220556

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  7. ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-hSHH

    Plasmid
    #179586
    Purpose
    ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette with SHH expression (Red module-dsRed/Green module-NeonGreen T2A SHH)
    Depositor
    Ali Hemmati Brivanlou
    Article
    De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:
    Insert
    NLS-NG-T2A-hSHH
    Use
    Piggybac
    Tags
    NeonGreen
    Promoter
    TRE
    Available Since
    Feb. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    179586-plasmid-map-sequence-id-354597

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  11. pCC_03 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9-NLS-2A-Puro-WPRE

    Plasmid
    #139088
    Purpose
    Expresses human codon-optimized xCas9 3.7 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    xCas9 3.7
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    A262T, R324L,S409I, E480K, E543D, M694I, E1219V
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139088-plasmid-map-sequence-id-273884

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  13. pCAG-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1065)

    Plasmid
    #114092
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a (S542R/K548V/N552R)
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    S542R, K548V and N552R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114092-plasmid-map-sequence-id-230539

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  15. pSB-CMV-MCS-Puro ArfGAP1_ALPS1_ALPS2-mKate2-3NLS-P2A-EGFP-LMNB1

    Plasmid
    #250908
    Purpose
    Dual color expression of mKate2-3NLS tagged human ARFGAP1 ALPS 1-2 and EGFP tagged zebrafish LaminB1 in mammallian cell lines
    Depositor
    Philipp Niethammer
    Article
    Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/
    Insert
    ArfGAP1-mKate2-3xNLS-P2A-EGFP-Lamin B1 (lmnb1, ARFGAP1 Zebrafish, Human)
    Tags
    EGFP on LMNB1 and mKate2-3xNLS on ArfGAP1
    Expression
    Mammalian
    Mutation
    ARFGAP1 amino acids 192–304 only
    Promoter
    CMV
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    250908-plasmid-map-sequence-id-498469

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  16. pSB-CMV-MCS-Puro-ArfGAP1_ALPS1-mKate2-3NLS-P2A-eGFP-LMNB1

    Plasmid
    #250914
    Purpose
    Dual color expression of mKate2-3xNLS tagged rat ALPS1 and eGFP tagged zebrafish Lamin B1 in mammalian cell lines
    Depositor
    Philipp Niethammer
    Article
    Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/
    Insert
    ArfGAP1-mKate2-3xNLS-P2A-EGFP-LMNB1 (lmnb1, Arfgap1 Zebrafish, Rat)
    Tags
    EGFP on LMNB1 and mKate2 on ArfGAP1
    Expression
    Mammalian
    Mutation
    ARFGAP1 amino acids 196–234 only
    Promoter
    CMV
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    250914-plasmid-map-sequence-id-498467

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Showing: 2161 - 2180 of 6478 results