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pCFJ1360 - Intron GG3 - smu-1

Plasmid

#159806

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG3 - smu-1

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ1358 - Intron GG1 - smu-1

Plasmid

#159804

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG1 - smu-1

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRB20

Plasmid

#52559

Purpose

serves as PCR-template to generate HR donors for C-terminal YFP-tag

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

eYFP and Blasticigin resistance cassette

Use

Blunt cloning kit

Available Since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

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TAL3344

Plasmid

#42807

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-prothymosin alpha a-left (ptmaa Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
RSPH1_pCSdest

Plasmid

#53926

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

RSPH1 (RSPH1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pDGE7

Plasmid

#79462

Purpose

sgRNA shuttle vector; module 2

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE9

Plasmid

#79465

Purpose

sgRNA shuttle vector; module 3

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE11

Plasmid

#79467

Purpose

sgRNA shuttle vector; module 4E

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ150-cul4

Plasmid

#80353

Purpose

Pmex-5:GFP::H2B::PEST::cul-4 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Article

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::cul-4 3'UTR

Expression

Worm

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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HOXA9 (murine) HIS-tag pET

Plasmid

#8492

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HoxA9 (Hoxa9 Mouse)

Tags

His and T7

Expression

Bacterial

Mutation

BamHI/BalI fusion into amino acid #2 of HOXA9; ma…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
PIGP_pCSdest

Plasmid

#53827

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

PIGP (PIGP Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
MIS12-FRB-FLAG

Plasmid

#114040

Purpose

Kinetochore localised FRB tagged with FLAG

Depositor

Geert Kops

Article

Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

Insert

MIS12 (MIS12 Human)

Tags

FRB-FLAG-tagRFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pBT241_(pCA-ATG-intron-tTA2)

Plasmid

#36875

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Insert

tTA2

Expression

Mammalian

Mutation

insertion of a beta-globin intron with a loxP sit…

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pAB031a

Plasmid

#79221

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ HA4-Zif268; PlacIq lacI

Expression

Bacterial

Promoter

PlacZ; PlacIq

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAB029f

Plasmid

#79220

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ lambda cI-SH2ABL1; PlacIq lacI

Expression

Bacterial

Promoter

PlacZ; PlacIq

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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TFF1_pCSdest

Plasmid

#53908

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

TFF1 (TFF1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
MIS18A_pCSdest

Plasmid

#53821

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

MIS18A (MIS18A Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pENTR4_FLAG/HA_EWSR1_His6

Plasmid

#26364

Depositor

Thomas Tuschl

Article

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Insert

EWSR1 (EWSR1 Human)

Use

Entry vector

Tags

FLAG/HA and His6

Mutation

delta 657 (stop codon)

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
PSMG1_pCSdest

Plasmid

#53884

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

PSMG1 (PSMG1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pAB043c

Plasmid

#79223

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ lambda cI-p38-alpha; PlacIq lacI

Expression

Bacterial

Promoter

PlacZ; PlacIq

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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