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Showing: 201 - 220 of 4413 results

  1. SOX2-P2A-tagBFP-HDR

    Plasmid
    #163751
    Purpose
    HDR knock-in template for tagging the human endogenous SOX2 gene with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.
    Depositor
    Jennifer Mitchell
    Article
    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.
    Insert
    SOX2 (SOX2 Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    P2A-tagBFP
    Expression
    Mutation
    Does not contain start codon to avoid random inte…
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    163751-plasmid-map-sequence-id-326711

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  5. AAVS1-TRE3G-SOX2-3xHA-P2A-tagBFP

    Plasmid
    #163701
    Purpose
    Dox-inducible SOX2-3xHA HDR knock-in cassette into the AAVS1 locus with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.
    Depositor
    Jennifer Mitchell
    Article
    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.
    Inserts
    SOX2-3xHA-P2A-tagBFP (SOX2 Human)
    rtTA
    T2-sgRNA
    Use
    AAV and CRISPR
    Tags
    3x-HA and P2A-tagBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and TRE3G
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    163701-plasmid-map-sequence-id-325288

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  6. pET21b(+)_SARS-CoV-2_3CLpro-Q306A

    Plasmid
    #177334
    Purpose
    bacterial expression of active SARS-CoV-2 3C-like proteinase
    Depositor
    Christopher Overall
    Article
    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Use
    Tags
    Factor Xa site-3xFlag-Myc-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available since
    Nov. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    177334-plasmid-map-sequence-id-348602

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  7. pET21b(+)_SARS-CoV-2_3CLpro-C145A-Q306A

    Plasmid
    #177335
    Purpose
    bacterial expression of inactive SARS-CoV-2 3C-like-C145A proteinase
    Depositor
    Christopher Overall
    Article
    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:
    Insert
    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Use
    Tags
    Factor Xa site-3xFlag-Myc-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available since
    Nov. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    177335-plasmid-map-sequence-id-348601

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  9. pET12a-HAL

    Plasmid
    #47097
    Depositor
    Marina Ramirez-Alvarado
    Insert
    HAL
    Use
    Tags
    ompT
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    47097-plasmid-map-sequence-id-215940

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  10. pET12a-JOH1

    Plasmid
    #47098
    Depositor
    Marina Ramirez-Alvarado
    Insert
    JOH1 (IGKV3-20 Human)
    Use
    Tags
    ompT
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  11. pET12a-DAH

    Plasmid
    #47096
    Depositor
    Marina Ramirez-Alvarado
    Insert
    DAH (IGKV3-20 Human)
    Use
    Tags
    ompT
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. pEXP5-pOsmB::mCherry

    Plasmid
    #53313
    Purpose
    mCherry reporter plasmid for OsmB promoter
    Depositor
    William DeGrado
    Insert
    OsmB (osmB E.coli)
    Use
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. pEXP5-pSoxS::mCherry

    Plasmid
    #53311
    Purpose
    mCherry reporter plasmid for SoxS promoter
    Depositor
    William DeGrado
    Insert
    SoxS promoter (soxS E.coli)
    Use
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 201 - 220 of 4413 results