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Plasmid#68233PurposeTwister plasmid to swap inserts from an alpha1 or alpha1R vector to any omega level vectorDepositorInsertSolanum lycopersicum intergenic region
UseSynthetic Biology; UnspecifiedTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYSD006
Plasmid#212903PurposeEncodes S. cerevisiae MFalpha1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD023
Plasmid#212909PurposeEncodes a hybrid S. cerevisiae OST1 pre- MFalpha1 pro- signal peptide (OST1MFapp) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD021
Plasmid#212907PurposeEncodes an engineered S. cerevisiae MFalpha1 pre-pro- signal peptide (MFapp8) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD024
Plasmid#212910PurposeEncodes the wildtype S. cerevisiae MFalpha1 pre-pro- signal peptide (MFappWT) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD022
Plasmid#212908PurposeEncodes an engineered S. cerevisiae MFalpha1 pre-pro- signal peptide (MFappOPT) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GABRA1-IRES2-EGFP
Plasmid#170820Purposehuman GABAA receptors alpha1 subunit and IRES2-EGFPDepositorInsertHuman GABAA receptor alpha1 subunit (GABRA1 Human)
UseTagsIRES2-EGFPExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
CaV2.1 (pcDNA6)
Plasmid#26578DepositorInsertCacna1a (Cacna1a Mouse)
UseTagsHis6 and V5ExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
Activin A (S2-2)
Plasmid#184621Purposefor transfection into S2 cells and expression of Activin A proteinDepositorInsertINHBA (INHBA Human)
UseTags8x His - streptavidin binding peptideExpressionInsectMutationPromoterAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBJ 2358
Plasmid#89245PurposeExpresses YFP fused to the N-terminus of Mouse Capping Protein Alpha1, using the Clontech vector PEYFP C-1.DepositorInsertCapza1 (Capza1 Mouse)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lck-mCherry-RGS
Plasmid#112935PurposePlasmid encodes a membrane located inhibitor of Ga12/13 signaling (RGS domain from p115)DepositorInsertp115RhoGEF-RGS domain
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
FB010
Plasmid#119708PurposeTranscriptional Unit (TU) for hygromicin resistance in fungi obtained by combining FB001+GB0211+FB002 into pDGB3alpha1. According to FungalBraid/GoldenBraid modular DNA assembly for ATMTDepositorInsertPtrpC:hph:Ttub
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
NES-EGFP-cPHx3
Plasmid#116855PurposePI(3,4)P2 biosensorDepositorInsertPLEKHA1 (PLEKHA1 Human)
UseTagsEGFP and X.leavis map2k1.L(32-44)ExpressionMammalianMutationamino acids 169-329 (tandem trimer)PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
NES-mCherry-cPHx3
Plasmid#116862PurposePI(3,4)P2 biosensorDepositorInsertPLEKHA1 (PLEKHA1 Human)
UseTagsmCherryExpressionMammalianMutationamino acids 169-329 (tandem trimer)PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
a1Ha-pcDNA3
Plasmid#45809DepositorInserta1Ha (CACNA1H Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP-2xPH-TAPP1 WT
Plasmid#161990PurposeExpresses two tandem repeats of Tapp1 PH domain that binds to PI(3,4)P2. Fused to eGFPDepositorInsertPH-TAPP1 (PLEKHA1 Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
NES-PAmCherry-cPHx3
Plasmid#116861PurposePI(3,4)P2 biosensorDepositorInsertPLEKHA1 (PLEKHA1 Human)
UseTagsPAmCherryExpressionMammalianMutationamino acids 169-329 (tandem trimer)PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GABRA1(A322D)-IRES2-EGFP
Plasmid#170821Purposehuman GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFPDepositorInsertHuman GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)
UseTagsIRES2-EGFPExpressionMammalianMutationchange Alanine 322 to AspartatePromoterAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
NES-EGFP-cPHx1
Plasmid#116853PurposePI(3,4)P2 biosensorDepositorInsertPLEKHA1 (PLEKHA1 )
UseTagsEGFP and X.leavis map2k1.L(32-44)ExpressionMammalianMutationAmino acids 169.329PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTub-alpha-EB3-NeonGreen
Plasmid#175263Purposelabelling of microtubule plus end and F-actin structuresDepositorInsertEB3 (MAPRE3 Human)
UseTagsmNeonGreenExpressionMammalianMutationNone (wt)PromoterpTub-alpha1Available sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only