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pCMV-Sport6-CD9-pHluorin
Plasmid#130905PurposeExpression of CD9-pHluorin for visualization of multivesicular body-plasma membrane fusion.DepositorInsertCD9-pHluorin (CD9 Aequorea victoria, Human)
UseTagspHluorinExpressionMammalianMutationphluorin inserted between Asn-50 and Asn-51 in ex…PromoterCMVAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-muOR-GFP11-T2A-GFP10-bArrestin1-T2A mCherry
Plasmid#113611PurposePPI reporterDepositorInsertmu opioid receptor
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS-21a his6-GST-WTPhi29DNAP
Plasmid#166863PurposeTo produce a dual affinity-tagged Phi29 DNA polymeraseDepositorInsertPhi29 Polymerase codon-optimized for overexpression in E. coli
UseTagsGST and his6ExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-BroccoliSamSensor#1
Plasmid#124363PurposeOverexpression of circular RNA (Broccoli-based SAM sensor)DepositorInsertTornado-BroccoliSamSensor#1
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2-nCas9n
Plasmid#47929Purposeexpression of an optimized Cas9 for genome-editing in zebrafishDepositorInsertnls-zcas9-nls
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28c-F30-Broccoli
Plasmid#66788PurposeExpresses Broccoli in the F30 scaffold in bacterial cellsDepositorInsertBroccoli in the F30 scaffold
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-gRNA
Plasmid#46759PurposegRNA cloning vector for in vitro transcription of target gRNADepositorInsertgRNA core
UseCRISPR; Zebrafish expressionTagsExpressionMutationPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LacI
Plasmid#46394PurposeOverproduction of LacIDepositorInsertLacI
UseTags6xHIS tagExpressionBacterialMutationsilent T to C mutation at position 19Promoterarabinose promoterAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-b2AR-GFP11-T2A-GFP10-bArrestin1-T2A mCherry
Plasmid#113610PurposePPI reporterDepositorInsertbeta 2 adrenergic receptor
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 V5 hHR23B
Plasmid#13054DepositorInserthHR23B (RAD23B Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAV5S-F30-2xdBroccoli
Plasmid#66845PurposeExpresses 5S rRNA fused to two dimeric Broccoli aptamers in the F30 scaffold in mammalian cellsDepositorInsertTwo dimeric Broccoli aptamers in the F30 scaffold
UseTags5S rRNAExpressionMammalianMutationPromoter5SAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
FusX DdCBE DddA11 1397N
Plasmid#221935PurposeBackbone plasmid for FusX-based assembly of DdCBE arm with DddA11 1397NDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGExpressionMutationDddA: S1330I, A1341V, N1342S, E1370K, T1380IPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FusX alphaDdCBE DddA6-11 1397C
Plasmid#221938PurposeBackbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA6/11 1397CDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGExpressionMutationTALE: Q231R, W232G, S233A | DddA: T1413IPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FusX alphaDdCBE DddA6 1397N
Plasmid#221939PurposeBackbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA6 1397NDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGExpressionMutationTALE: Q231R, W232G, S233A | DddA: Q1310R, S1330I,…PromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FusX alphaDdCBE DddA11 1397N
Plasmid#221940PurposeBackbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA11 1397NDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGExpressionMutationTALE: Q231R, W232G, S233A | DddA: S1330I, A1341V,…PromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FusX DdCBE DddA6-11 1397C
Plasmid#221933PurposeBackbone plasmid for FusX-based assembly of DdCBE arm with DddA6/11 1397CDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGExpressionMutationDddA: T1413IPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only
