We narrowed to 10,463 results for: T7

1. QUAS-0-T7-TetO-GFP_ColE1

   Plasmid

   #171662

   Purpose

   QUAS directly upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli. Contains the ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   QUAS-T7-TetO-GFP ssrA-T7 Stop
   

   Use

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET30-T7-lacO-His-sfGFP

   Plasmid

   #180754

   Purpose

   pET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminator

   Depositor

   Michael Black

   Article

   Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

   Insert

   superfolder green fluorescent protein
   

   Use

   Tags

   strep tag

   Expression

   Bacterial

   Mutation

   G140C- Please see depositor comments

   Promoter

   Available since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA T7-Gnat1 Mut-3UTR

   Plasmid

   #184027

   Purpose

   Expresses mouse Gnat1 protein with N-terminal T7 tag from cDNA that contains the mutant 3'-UTR where the TAG binding sites for MSI1 are mutated to TGA

   Depositor

   Peter Stoilov

   Article

   Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

   Insert

   Gnat1 (Gnat1 Mouse)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   TAG sites in the 3'-UTR are mutated to TGA

   Promoter

   CMV

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-20b-T7-Ntx-K49

   Plasmid

   #58715

   Purpose

   Expresses in Escherichia coli T7-Notexin with the amino acid substitution D49K

   Depositor

   Fiorella Tonello

   Article

   Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

   Insert

   Notexin
   

   Use

   Tags

   T7 peptide tag

   Expression

   Bacterial

   Mutation

   Changed Aspartic Acid 49 to Lysine

   Promoter

   T7

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3 T7 Jip1b(471-707)

   Plasmid

   #52428

   Purpose

   a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

   Depositor

   Roger Davis

   Article

   Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

   Insert

   JNK interacting protein-1b (JIP-1b)(471-707) ) (Mapk8ip1 Mouse)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   deleted amino acid 1-470

   Promoter

   Available since

   April 29, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pcdna3 T7 Jip1b(282-470)

   Plasmid

   #52125

   Purpose

   a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

   Depositor

   Roger Davis

   Article

   Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

   Insert

   JNK interacting protein-1b (JIP-1b)(282-470) (Mapk8ip1 Mouse)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   deleted amino acid 1-281 and 471-707

   Promoter

   Available since

   April 18, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pcdna3 T7 Jip1b(282-707)

   Plasmid

   #52126

   Purpose

   a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

   Depositor

   Roger Davis

   Article

   Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

   Insert

   JNK interacting protein-1b (JIP-1b)(282-707)) (Mapk8ip1 Mouse)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   deleted amino acid 1-281

   Promoter

   Available since

   April 18, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pCDNA3 T7 Jip1(1-470)

   Plasmid

   #51724

   Purpose

   " JIP-1 may suppress signal transduction by the JNK pathway"

   Depositor

   Roger Davis

   Article

   Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

   Insert

   Jnk interacting protein 1 (Mapk8ip1 Mouse)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   deleted 471-660

   Promoter

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA3 T7 Jip1(471-660)

   Plasmid

   #51766

   Purpose

   " JIP-1 may suppress signal transduction by the JNK pathway"

   Depositor

   Roger Davis

   Article

   Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

   Insert

   Jnk interacting protein 1 (Mapk8ip1 Mouse)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   deleted 1-470

   Promoter

   Available since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pCDNA3 T7 Jip1(1-281)

    Plasmid

    #51700

    Purpose

    " JIP-1 may suppress signal transduction by the JNK pathway"

    Depositor

    Roger Davis

    Article

    Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

    Insert

    Jnk interacting protein 1 (Mapk8ip1 Mouse)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted 282-660

    Promoter

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pCDNA3 T7 BimL(S58D,DE)

    Plasmid

    #25057

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    BimL (S58D,DE) (BCL2L11 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    Changed serine 58 to aspartic acid changed leuci…

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pCDNA3 T7 BimL(S44A,DE)

    Plasmid

    #25054

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    BimL (S44A,DE) (BCL2L11 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    Changed serine 44 to alanine changed leucine 92 …

    Promoter

    Available since

    Aug. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pCDNA3 T7 BimL(T56A,DE)

    Plasmid

    #25055

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    BimL (T56A,DE) (BCL2L11 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    Changed threonine 56 to alanine changed leucine …

    Promoter

    Available since

    Aug. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pCDNA3 T7 BimL(S58A,DE)

    Plasmid

    #25056

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    BimL (S58A,DE) (BCL2L11 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    Changed serine 58 to alanine changed leucine 92 …

    Promoter

    Available since

    Aug. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pPGK-T7/2-CD44v6-10 (fl)

    Plasmid

    #137820

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v6-10 (fl) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    full length

    Promoter

    PGK

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

    Plasmid

    #139989

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid

    #139987

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-28a(+)-T7-OmpA-MNaseA-6xHis

    Plasmid

    #214808

    Purpose

    Expression of 6xhis-tagged micrococcal nuclease A (MNaseA)

    Depositor

    Peter Sarin

    Article

    Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

    Insert

    OmpA-Micrococcal nuclease A
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Polymerase expression - pCMV-T7-EcKlenow (LM2692)

    Plasmid

    #208963

    Purpose

    Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    EcKlenow-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 1013 pcDNA3 T7 Akt1 T308A S473A

    Plasmid

    #9030

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    Akt1 T308A S473A (AKT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T308A S473A

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge