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We narrowed to 2,357 results for: buc

Showing: 201 - 220 of 2357 results

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pCS2-mCherry_SNAP_SNAPV_PACT_Dead

Plasmid

#173073

Purpose

Dead SNAP tag at the centrosome

Depositor

Heidi Hehnly

Article

Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

Insert

mCherry-SNAP-SNAP-Varied-PACT

Use

Tags

mCherry-SNAP-SNAP-Varied

Expression

Mammalian

Mutation

AA144 in both SNAP tags changed from a cysteine t…

Promoter

CMV IE94

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pJOE5751.1

Plasmid

#135083

Purpose

pBR322-based L-rhamnose-inducible vector.

Depositor

Josef Altenbuchner

Article

Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

Insert

P-rhaP-BAD-His tag-eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBW313lux-hrpR

Plasmid

#61436

Purpose

pBWF2620 encoding rbs33-hrpR-ter (pBWF2620: pSB3K3 carrying BBa_F2620 (PTet-rbs34-luxR-ter))

Depositor

Martin Buck, Baojun Wang

Article

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

F2620-rbs33-hrpR-ter

Use

Tags

Expression

Bacterial

Mutation

Promoter

plux

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pNB784

Plasmid

#60163

Purpose

Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus

Use

Tags

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

SIC1pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pEM01

Plasmid

#135414

Purpose

Expression of Hygromycin resistance from CMV promoter

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

CMVpr hph ADH1t

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

CMVpr

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCDB368

Plasmid

#110276

Purpose

gHH_44 with a N-terminal 10-His DsbC TEV-peptide fusion

Depositor

Christopher Bahl

Article

Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

Insert

gHH_44

Use

Tags

10-His, Tev recognition peptide, and mature DsbC …

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pJOE6089.4

Plasmid

#135081

Purpose

pBR322-based L-rhamnose-inducible vector.

Depositor

Josef Altenbuchner

Article

Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

Insert

P-rhaP-BAD-eGFP-Strep-tag II

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pNB695

Plasmid

#60144

Purpose

Disintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

GrLuc

Use

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

Dec. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pNB697

Plasmid

#60146

Purpose

Disintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

RdLuc

Use

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

Dec. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pNB788

Plasmid

#60165

Purpose

Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by RNR1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus

Use

Tags

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

RNR1pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pOP823

Plasmid

#235718

Purpose

Protein expression of His6-SUMO-HsRAB5A (Q79L C19S,C63S) 1-212 in bacteria for maleimide conjugation

Depositor

Roger Williams

Article

Buckles et al Biophys J. 2020 Dec 1;119(11):2205-2218.

Insert

RAB5A (RAB5A Human)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

Q79L, C19S, C63S, aa 1-212

Promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1645

Plasmid

#235719

Purpose

Protein expression of GST-tagged PX domain (Cys at 2 and C85S) in bacteria

Depositor

Roger Williams

Article

Buckles et al Biophys J. 2020 Dec 1;119(11):2205-2218.

Insert

p40phox PX domain (NCF4 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Cys at 2, C85S, aa 2-149

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Vibrio_vulnificus

Plasmid

#224405

Purpose

For expression of the type I CBASS system encoded by Vibrio vulnificus

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

Endonuclease_Phospholipase_VvCdnG_AGS-C

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Vibrio_campbellii

Plasmid

#224404

Purpose

For expression of the type I CBASS system encoded by Vibrio campbellii

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

Endonuclease_VcCdnG_AGS-C

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Pseudoalteromonas_sp._A2

Plasmid

#224401

Purpose

For expression of the type I CBASS system encoded by Pseudoalteromonas sp. A2

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

Psp.CdnB_AGS-C_Cap15

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Pectobacterium_parmentieri

Plasmid

#224400

Purpose

For expression of the type I CBASS system encoded by Pectobacterium parmentieri

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

PpCdnB_AGS-C_Cap15

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Alteromonas_sp._ALT199

Plasmid

#224399

Purpose

For expression of the type I CBASS system encoded by Alteromonas sp. ALT199

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

Asp.CdnB_AGS-C_Cap15

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Rhodanobacter_denitrificans

Plasmid

#224391

Purpose

For expression of the type I CBASS system encoded by Rhodanobacter denitrificans

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

Phospholipase_RdCdnA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGI3EM11

Plasmid

#135483

Purpose

Agrobacterium T-DNA with SpunH2Bpr driving hph-GFP

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunH2Bpr hph-GFP ADH1t

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Promoter

SpunH2Bpr

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGI3EM29

Plasmid

#135489

Purpose

Agrobacterium T-DNA with Spun H2Bpr driving hph-mCitrine

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr hph-mCitrine

Use

Tags

mCitrine

Expression

Bacterial and Yeast

Mutation

Promoter

SpunH2A/Bpr (divergent)

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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