We narrowed to 29,548 results for: des.2

1. pS44i8GH-2

   Plasmid

   #207526

   Purpose

   Conditionally-replicating in Pseudomonas vector, high-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; SmR/SpR

   Depositor

   Pablo Ivan Nikel

   Article

   Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

   Insert

   oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP
   

   Use

   CRISPR; Pseudomonas vector

   Available Since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFAP4_BHLH_3-2

   Plasmid

   #185055

   Purpose

   Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_5-1 or TFAP4_BHLH_5-2

   Depositor

   Constance Ciaudo

   Article

   Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

   Insert

   TFAP4_3_2_gRNA (Tcfap4 Mouse)
   

   Use

   Lentiviral

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFAP4_BHLH_5-2

   Plasmid

   #185056

   Purpose

   Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_3-2

   Depositor

   Constance Ciaudo

   Article

   Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

   Insert

   TFAP4_5_2_gRNA (Tcfap4 Mouse)
   

   Use

   Lentiviral

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. AP334-2

   Plasmid

   #66095

   Purpose

   co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

   Insert

   sgRNA for APs5
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6

   Available Since

   July 2, 2015

   Availability

   Academic Institutions and Nonprofits only

5. AP324-2

   Plasmid

   #66092

   Purpose

   co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

   Insert

   sgRNA for APs6
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6

   Available Since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

6. AP332-2

   Plasmid

   #66089

   Purpose

   co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

   Insert

   sgRNA for APs4
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6

   Available Since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pCB-2

   Plasmid

   #210388

   Purpose

   All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

   Depositor

   Gilles van Wezel

   Article

   Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

   Inserts

   dCas9
   CymR
   sgRNA cassette
   

   Use

   CRISPR; Integrative vector for use in streptomyces

   Mutation

   BsaI-flanked RFP cassette inserted in place of sp…

   Promoter

   SP11 and SP30

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFNC-2

   Plasmid

   #227667

   Purpose

   Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. KmR, easily curable via sucrose counterselection.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Insert

   KmR
   

   Expression

   Bacterial

   Available Since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCIFR-2

   Plasmid

   #227663

   Purpose

   Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Km) used for selecting for the integration can be removed in a later stage with pFNC.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Insert

   msfGFP
   

   Expression

   Bacterial

   Promoter

   p14G-BCD2

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Control-2

    Plasmid

    #204443

    Purpose

    Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

    Depositor

    Keir Neuman

    Article

    Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

    Insert

    hOpa1 isoform 1 (OPA1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L248A

    Promoter

    CMV

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pORTMAGE-2

    Plasmid

    #72677

    Purpose

    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.

    Depositor

    Csaba Pál

    Article

    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

    Insert

    mutL E32K
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    E32K mutation conferring dominant mutator phenoty…

    Promoter

    pL promoter

    Available Since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRDA355_sgCiPELO #2

    Plasmid

    #229020

    Purpose

    Expression of a CRISPRi doxycycline inducible guide targeting PELO

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    PELO gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. shFGF2#2

    Plasmid

    #157668

    Purpose

    Tet inducible knockdown of FGF2

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Fibroblast growth factor 2 (FGF2 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. L2E-2

    Plasmid

    #21194

    Depositor

    Paul Khavari

    Article

    Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

    Insert

    Mek2 (Esr1, MAP2K2 Human, Mouse)
    

    Use

    Retroviral

    Mutation

    deletion of residues EQQKKRLE, S222D S226D in Me…

    Available Since

    July 24, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pRDA052H_sgFOCAD #2

    Plasmid

    #229024

    Purpose

    Expression of a EnAsCas12a guide targeting FOCAD

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    FOCAD gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXPR_051d_sgCiChr2-2

    Plasmid

    #228940

    Purpose

    Expression of a CRISPRi control sgRNA that cuts an intergenic region on chromosome 2

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    sgChr2-2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Interface-2

    Plasmid

    #204433

    Purpose

    Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

    Depositor

    Keir Neuman

    Article

    Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

    Insert

    hOpa1 isoform 1 (OPA1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K614A H615A D835A

    Promoter

    CMV

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSPgRNA_BLK_E2-2

    Plasmid

    #124475

    Purpose

    For CRISPR-mediated epigenome editing of human BLK gene locus

    Depositor

    Iouri Chepelev

    Article

    Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

    Insert

    BLK_E2-2_gRNA (BLK Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. shAPI5#2

    Plasmid

    #157666

    Purpose

    Tet inducible knockdown of API5

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Apoptosis inhibitor 5 (API5 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO_FOXA1_#2

    Plasmid

    #70096

    Purpose

    Expression of shRNA to human FOXA1, puromycin selection

    Depositor

    William Hahn

    Article

    Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

    Insert

    FOXA1 (FOXA1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    u6

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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