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pASKt15C+SrtA

Plasmid

#64983

Purpose

Expresses the wild type of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

His6 tag and S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

tetracycline promoter/operator

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pGK415-rtetTAK8N, L131L

Plasmid

#165969

Purpose

Expresses rTetTA (K8N, L131L) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

tetR

Use

Synthetic Biology

Tags

three copies of VP16 (VP48)

Expression

Yeast

Promoter

pPGK1

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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Kohinoor-Tublin/pcDNA3

Plasmid

#67780

Purpose

Tublin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

b-Tubulin

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only
pY1

Plasmid

#50597

Purpose

Backbone: pRBS-01, Origin Of Replication: p15a, Selection Markers: Amp, Promoters: PLacUV5,

Depositor

Jay Keasling

Article

Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8

Type

Empty backbone

Use

Synthetic Biology; Empty vector

Expression

Bacterial

Promoter

PLacUV5

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only
Kohinoor-Clathrin

Plasmid

#67773

Purpose

Clathrin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

Clathrin

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2015

Availability

Academic Institutions and Nonprofits only
YeNL-H2B_pcDNA3

Plasmid

#89522

Purpose

Yellow color variant of bright luminescent protein, fused to histone 2B

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

YeNL

Tags

histone 2B

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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Kohinoor-mitochondria/pcDNA3

Plasmid

#67778

Purpose

Mitochondria-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

CoxVIII x 2

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
Kohinoor-H2B/pcDNA3

Plasmid

#67774

Purpose

Histon2B-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

H2B

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2015

Availability

Academic Institutions and Nonprofits only
tubulin-CeNL_pcDNA3

Plasmid

#89515

Purpose

Cyan color variant of bright luminescent protein, fused to b-tubulin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Tags

b-tubulin

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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YeNL-Actin_pcDNA3

Plasmid

#89521

Purpose

Yellow color variant of bright luminescent protein, fused to actin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

YeNL

Tags

Actin

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pET28b-MseLig1

Plasmid

#107071

Purpose

Expresses M. sedula DNA Ligase 1 in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

DNA ligase 1

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBsaHFm

Plasmid

#85103

Purpose

Heme-FMN domain of P450 BM3 variant (A74G)

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

Heme-FMN domain of P450 BM3

Use

Cloning vector

Mutation

A74G

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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OeNL-H2B_pcDNA3

Plasmid

#89527

Purpose

Orange color variant of bright luminescent protein, fused to histone 2B

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Tags

histone 2B

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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Kohinoor-Golgi/pcDNA3

Plasmid

#67779

Purpose

Golgi-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

GT

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only
Kohinoor-Zyxin/pcDNA3

Plasmid

#67777

Purpose

Zyxin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

Zyxin

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only
OeNL-Actin_pcDNA3

Plasmid

#89524

Purpose

Orange color variant of bright luminescent protein, fused to actin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Tags

Actin

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pET11a+D77R/T214V

Plasmid

#66135

Purpose

Expresses the D77R/T214V mutant of CYP119

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Expression

Bacterial

Mutation

D77R, T214V

Promoter

T7 promoter

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b-YC

Plasmid

#107072

Purpose

Host vector to construct a YPer or CyPet fusion protein

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

YPet-CyPet fusion protein

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA2

Plasmid

#64984

Purpose

Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

His6 tag and S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108A

Promoter

tetracycline promoter/operator

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtAwoH

Plasmid

#65017

Purpose

Expresses the wild type of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

tetracycline promoter/operator

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge