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We narrowed to 215 results for: pichia pastoris

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Showing: 201 - 215 of 215 results

  1. pPICZ A-SLC-His+MFN2[∆TM]-Strep (SB260)

    Plasmid
    #227609
    Purpose
    Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region in Pichia pastoris
    Depositor
    Luke Chao
    Article
    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10
    Inserts
    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    Tags
    10xHis and Twin-StrepTag
    Expression
    Yeast
    Mutation
    contains aa 2-544, 651-757 only [TM deleted]
    Promoter
    AOX1
    Available Since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227609-plasmid-map-sequence-id-455572

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  3. pPICZ A-SLC(2-88)-MBP-His+MFN2-Strep (SB262)

    Plasmid
    #227611
    Purpose
    Inducible coexpression of His-tagged SLC25A46 truncated to its N-terminal region (2-88) fused to MBP and MFN2-Strep in Pichia pastoris
    Depositor
    Luke Chao
    Article
    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10
    Inserts
    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    Tags
    MBP, 10xHis and Twin-StrepTag
    Expression
    Yeast
    Mutation
    contains aa 2-88 only
    Promoter
    AOX1
    Available Since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227611-plasmid-map-sequence-id-455570

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  4. pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261)

    Plasmid
    #227610
    Purpose
    Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region and helical bundle 2 (HB2) in Pichia pastoris
    Depositor
    Luke Chao
    Article
    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10
    Inserts
    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    Tags
    10xHis and Twin-StrepTag
    Expression
    Yeast
    Mutation
    contains aa 2-400, 706-757 [TM and HB2 deleted]
    Promoter
    AOX1
    Available Since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227610-plasmid-map-sequence-id-455569

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  6. pPICZ:K2P2.1(mTREK-1)Cryst S112C

    Plasmid
    #224780
    Purpose
    Pichia pastoris expression vector for generating mouse TREK-1(21S-322T) + 3C cleavable site + eGFP + 10x His under the AOX1 promoter
    Depositor
    Dan Minor
    Article
    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1
    Insert
    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    Expression
    Yeast
    Mutation
    K84R / Q85E / T86K / I88L / A89R / Q90A / A92P / …
    Promoter
    AOX1 promoter
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    224780-plasmid-map-sequence-id-445194

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  7. pX131

    Plasmid
    #167149
    Purpose
    MoClo-compatible Level 0-SP promoterless vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    4'-phosphopantetheinyl transferase, npgA
    Use
    Synthetic Biology
    Available Since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167149-plasmid-map-sequence-id-335232

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  8. pX018

    Plasmid
    #167144
    Purpose
    MoClo-compatible Level 0-SP promoterless vector encoding Neonothopanus nambi luciferase nnLuz codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    fungal luciferase, nnLuz
    Use
    Luciferase and Synthetic Biology
    Available Since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    167144-plasmid-map-sequence-id-335106

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  9. Yeast Secrete and Detect

    Plasmid Kit
    #1000000166
    Purpose
    Assembly of transcription units for protein secretion in the yeasts Saccharomyces cerevisiae and Pichia pastoris.
    Depositor
    Sylvestre Marillonnet
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  10. pNK2657

    Plasmid
    #219741
    Purpose
    MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    hispidin- synthase from Mycena citricolor
    Use
    Synthetic Biology
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219741-plasmid-map-sequence-id-437095

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  11. pNK4169

    Plasmid
    #219744
    Purpose
    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Pichia pastoris
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    mutant of fungal hispidin-3-hydroxylase
    Use
    Synthetic Biology
    Mutation
    D37E, V181I, S323M, M385K
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219744-plasmid-map-sequence-id-437109

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  12. pNK845

    Plasmid
    #219746
    Purpose
    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Pichia pastoris, Homo sapiens
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    mutant of fungal luciferase
    Use
    Luciferase and Synthetic Biology
    Mutation
    I3S, N4T, F11L, I63T, T99P, T192S, A199P
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219746-plasmid-map-sequence-id-437111

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  13. pCri System

    Plasmid Kit
    #1000000058
    Purpose
    Plasmids used for cytoplasmic and periplasmic/extracellular heterologous protein expression in E. coli, Bacillus subtilis, and Pichia pastoris.
    Depositor
    F Xavier Gomis-Ruth
    Application
    Gene Expression and Labeling
    Vector Type
    Bacterial Expression, Yeast Expression
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. pSF18

    Plasmid
    #222593
    Purpose
    BB2 ZWF1 tet-on overexpression cassette
    Depositor
    Matthias Steiger
    Article
    Matthias et al PLOS One
    Insert
    ZWF1
    Expression
    Bacterial
    Mutation
    Internal BsaI and BpiI sites removed from insert
    Promoter
    tet-on
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    222593-plasmid-map-sequence-id-443809

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  15. pSF522

    Plasmid
    #222588
    Purpose
    BB3 ZWF1 tet-on overexpression cassette with homologous flanks for integration at pyrG locus
    Depositor
    Matthias Steiger
    Article
    Matthias et al PLOS One
    Insert
    ZWF1
    Use
    A. niger
    Expression
    Bacterial
    Mutation
    Internal BsaI and BpiI sites removed from insert
    Promoter
    tet-on
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    222588-plasmid-map-sequence-id-450454

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Showing: 201 - 215 of 215 results