We narrowed to 1,079 results for: transfection

1. pUCHR-inLuc

   Plasmid

   #58955

   Purpose

   HIV-1 transfer vector, encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is silent in the transfected cells and expressed in the infected cells.

   Depositor

   Dmitriy Mazurov, David Derse

   Article

   Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788.

   Insert

   inLuc
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pARv-RFP

   Plasmid

   #60021

   Purpose

   pARv-RFP is a fluorescent reporter plasmid, that allows fast and easy evaluation of ZFN, TALEN or CRISPR/Cas9 activity in the transfected cells.

   Depositor

   Radislav Sedláček

   Article

   Kasparek et al FEBS Lett. 2014 Sep 18. pii: S0014-5793(

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Nov. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMPRA1

   Plasmid

   #49349

   Purpose

   Basic backbone for transient transfection of Massively Parallel Reporter Assay (MPRA) libraries

   Depositor

   Tarjei Mikkelsen

   Article

   Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-GluA1-scFV-Halo

   Plasmid

   #230065

   Purpose

   Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag. Can be used to produce scFv in HEK cells or directly transfected into neurons.

   Depositor

   Matthew Kennedy

   Insert

   Anti-GluA1 single chain variable fragment (scFv)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   Chicken beta actin (CAG)

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCSmp38ERT(#143)

   Plasmid

   #184077

   Purpose

   cyclofen-inducible P38 activation via mammalian cell transfection or mRNA synthesis

   Depositor

   Sophie Vriz

   Insert

   5myc-ZfP38a-ERT2
   

   Use

   Synthetic Biology

   Tags

   5xMyc

   Expression

   Mammalian

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   sCMV+SP6

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-SNAP-hCB1_IRES_EGFP

   Plasmid

   #223505

   Purpose

   Expression of CB1 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection marker

   Depositor

   James Frank

   Insert

   SNAP-tag-CNR1 and EGFP (CNR1 Synthetic, Human)
   

   Tags

   SNAP-tag

   Expression

   Mammalian

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSFV3-lacZ

   Plasmid

   #92074

   Purpose

   This is an expression plasmid for production of recombinant genomic SFV RNA encoding beta-galactosidase.

   Depositor

   Henrik Garoff

   Article

   Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

   Insert

   lacZ
   

   Use

   Lacz expression plasmid, for run-off transcriptio…

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMVTO-BFP

   Plasmid

   #179651

   Purpose

   BFP co-transfection marker

   Depositor

   Xiaojing Gao

   Article

   Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

   Insert

   BFP
   

   Expression

   Mammalian

   Available Since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PGK-mCherry

   Plasmid

   #236119

   Purpose

   Fluorescent transfection marker encoding mCherry under PGK promoter

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Insert

   mCherry
   

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-GluA1-scFv-Halo

    Plasmid

    #230055

    Purpose

    AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 fused to halotag. Can be directly transfected into neurons or used to prepare AAV expressing GluA1-scFv-Halo.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA1 single chain variable fragment (scFv)
    

    Use

    AAV

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    human synapsin

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC1809_pmax-RBM38-FRB

    Plasmid

    #118642

    Purpose

    Transient transfection; Expresses RBM38-FRB; CAGGS promoter

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    RBM38-FRB
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAC1810_pmax-FRB-RBM38

    Plasmid

    #118643

    Purpose

    Transient transfection; Expresses FRB-RBM38; CAGGS promoter

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    FRB-RBM38
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCSZPKIamutHAERT(#119)

    Plasmid

    #184076

    Purpose

    cyclofen-inducible PK inhibitor alpha (mut) activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Insert

    ZfmutPKAinhiba-HA-ERT2
    

    Use

    Synthetic Biology

    Tags

    internal tag: HA

    Expression

    Mammalian

    Mutation

    ZfPKIa: R19A, R20A; ERT2: G400V, M543A, L544A, de…

    Promoter

    sCMV+SP6

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSZPKIaHAERT(#118)

    Plasmid

    #184075

    Purpose

    cyclofen-inducible PK inhibitor alpha (wt) activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Insert

    ZfPKAinhiba-HA-ERT2
    

    Use

    Synthetic Biology

    Tags

    internal tag: HA

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSm5En2DER4(#1062)

    Plasmid

    #184093

    Purpose

    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Insert

    5myc-chkEn2(C>D)-ERT2
    

    Use

    Synthetic Biology

    Tags

    5xMyc

    Expression

    Mammalian

    Mutation

    chkEn2: C175D; ERT2: G400V, M543A, L544A, deleted…

    Promoter

    sCMV+SP6

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. C205

    Plasmid

    #174359

    Purpose

    For expressing siRNA-resistant frog ePAB in human cell lines via transfection

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    ePAB
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Multiple silent mutations introduced to be resist…

    Availability

    Academic Institutions and Nonprofits only

    

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17. Guzit

    Bacterial Strain

    #196902

    Purpose

    Guzit is a RNase HI(rnhA) His-tagged E. coli strain made for a cell-free expression system.

    Depositor

    Kate Adamala

    Article

    Sato et al Biotechnol Bioeng. 2023 Jul;120(7):1986-

    Bacterial Resistance

    Kanamycin

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

18. RNase III-His

    Bacterial Strain

    #196903

    Purpose

    RNase III-His is an E. coli strain made for a cell-free expression system.

    Depositor

    Kate Adamala

    Article

    Sato et al Biotechnol Bioeng. 2023 Jul;120(7):1986-

    Bacterial Resistance

    Kanamycin

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

19. pAC1801_pmax-dCasRx

    Plasmid

    #118634

    Purpose

    Transient transfection; Expresses dCasRx; CAGGS promoter

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    dCasRx
    

    Expression

    Mammalian

    Mutation

    R239A/H244A/R858A/H863A

    Promoter

    CAGGS

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLPCX-IRES-GFP

    Plasmid

    #65436

    Purpose

    For transfection or retroviral production. Mammalian expression of EGFP driven by the ECMV IRES.

    Depositor

    Trond Aasen

    Article

    Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

    Insert

    IRES-GFP
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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