We narrowed to 7,220 results for: 11

1. pReporter_3

   Plasmid

   #60564

   Purpose

   A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Reporter_3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pReporter_4

   Plasmid

   #60565

   Purpose

   A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Reporter_4
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBN1

   Plasmid

   #86710

   Purpose

   C. elegans injection marker

   Depositor

   Peter Askjaer

   Article

   Rodenas et al Mol Biol Cell. 2012 Mar;23(5):930-44. do

   Insert

   Plmn-1::mCherry::his-58::pie-1 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS1479-HTP-KanMX6

   Plasmid

   #154085

   Purpose

   For C-terminal his6-TEV-ProtA-tagging in S. pombe using KanMX6 as resistance marker.

   Depositor

   Lidia Vasiljeva

   Article

   Kilchert et al Cell Rep. 2015 Dec 22;13(11):2504-2515.

   Insert

   his6-TEV-protein A-KanR
   

   Use

   Pcr-based tagging in yeast

   Tags

   his6-TEV-protein A

   Expression

   Mutation

   Promoter

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUW-tetO-hSOX2

   Plasmid

   #20724

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

   Insert

   SOX2 (SOX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSP1E3D1_D73C

   Plasmid

   #39328

   Depositor

   Stephen Sligar

   Article

   Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

   Insert

   MSP1E3D1_D73C (APOA1 synthetic gene)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   cys mutant of MSP1E3D1

   Promoter

   T7

   Available since

   Sept. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

7. HBV 1.3-mer P-null replicon

   Plasmid

   #65462

   Purpose

   containing 1.3 units of the P-null HBV genome (subtype ayw)

   Depositor

   Wang-Shick Ryu

   Article

   Wang et al J Virol. 2009 Jun;83(11):5815-24. doi: 1

   Insert

   1.3 units of the P-null HBV genome
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. DYSF-3HA

   Plasmid

   #29767

   Depositor

   Steven Vogel

   Article

   Covian-Nares et al J Cell Sci. 2010 Jun 1. 123(Pt 11):1884-

   Insert

   DYSF (DYSF Human)
   

   Use

   Tags

   3xHA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSC234

   Plasmid

   #216357

   Purpose

   xhNup93-Nb3t (+2Cys) Nanobody production

   Depositor

   Dirk Görlich

   Article

   Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

   Insert

   xhNup93-Nb3t (+2Cys)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   14xHis-NEDD8

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-His6-GFP-C-LACE

    Plasmid

    #133913

    Purpose

    Bacterial expression of His6-GFP-C-LACE

    Depositor

    Jeffrey Bode

    Article

    Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

    Insert

    superfolder green fluorescent protein
    

    Use

    Tags

    LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEasy Flox

    Plasmid

    #11725

    Depositor

    Klaus Rajewsky

    Article

    Schenten et al Eur J Immunol. 2002 Nov . 32(11):3152-60

    Insert

    pEasyFlox
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC0096 RESCUE truncation (Cas13 delta 892-1095)

    Plasmid

    #130663

    Purpose

    CMV-dCas13b6(delta 892-1095)-mapkNES-GS-RESCUE

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

    Insert

    dRanCas13b(delta 892-1095)-ADAR2dd
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEBG-hUBP43

    Plasmid

    #12455

    Depositor

    Dong-Er Zhang

    Article

    Malakhova et al EMBO J. 2006 Jun 7. 25(11):2358-67.

    Insert

    UBP43 (USP18 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    pEBG vector is from Bruce Mayer, may be different…

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pET-21a(+)-AK1-His

    Plasmid

    #118977

    Purpose

    Bacterial expression plasmid for Adenylate kinase isoenzyme 1

    Depositor

    Sebastian Maerkl

    Article

    Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

    Insert

    adenylate kinase isoenzyme 1 AK1 (AK1 Chicken)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSP1E1D1

    Plasmid

    #161605

    Purpose

    Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

    Insert

    MSP1E1D1
    

    Use

    Tags

    7-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1D1; contains repeat of h…

    Promoter

    T7

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLH-sgRNA-Sirius-8XMS2

    Plasmid

    #121939

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-8XMS2
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p3868 HA-E6-AP

    Plasmid

    #8648

    Depositor

    Peter Howley

    Article

    Talis et al J Biol Chem 1998 Mar 13;273(11):6439-45.

    Insert

    E6-AP (UBE3A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCI SMN1

    Plasmid

    #72286

    Purpose

    SMN1 exon 7 splicing cassette

    Depositor

    Elliot Androphy

    Article

    Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

    Insert

    SMN1 (SMN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    exons 6-7-8 including introns

    Promoter

    CMV

    Available since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pCAG-3xGFP11-mem-3xControlgRNA

    Plasmid

    #224583

    Purpose

    Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    3xGFP11
    

    Use

    CRISPR

    Tags

    Membrane Localization Signal

    Expression

    Mutation

    Codon optimized

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX459-HypaCas9-mR2-AAVS1_sgRNA

    Plasmid

    #183890

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    AAVS1 sgRNA spacer (AAVS1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge