We narrowed to 8,936 results for: Arch

1. pLKO-CreERT2 stuffer v3

   Plasmid

   #158046

   Purpose

   lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pEGFP-C1-BBS2

   Plasmid

   #218712

   Purpose

   Expresses N-terminally EGFP-tagged BBS2 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

   Insert

   BBS2 (BBS2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDARMO_HUWE1_3xFLAG

   Plasmid

   #187155

   Purpose

   mammalian cell expression of HUWE1-3xFLAG

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe puro c-met WT

   Plasmid

   #17493

   Depositor

   Joan Brugge

   Article

   Wrobel et al J Cell Biol. 2004 Apr 26. 165(2):263-73.

   Insert

   c-Met (Met Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST_FLAG-HUWE1

   Plasmid

   #187120

   Purpose

   mammalian cell expression of FLAG-HUWE1

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Tags

   1xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSF-G1TFAKRS

   Plasmid

   #177311

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(trifluoroacetyl)-L-lysine (TFA-Lys) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Orton et al J Am Chem Soc. 2021 Nov 15. doi: 10.1021

   Insert

   tRNA synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti Lifeact-EGFP BlastR

   Plasmid

   #84383

   Purpose

   Lentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neurons

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Insert

   Lifeact
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   CMV immediate early

   Available since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpRY Cas9

   Plasmid

   #188491

   Purpose

   Expresses FLAG tagged SpRY Cas9

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

   Promoter

   EF1a

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA-FLAG-METTL3

   Plasmid

   #160250

   Purpose

   Express FLAG-METTL3 in human cells

   Depositor

   Alessandro Fatica

   Article

   Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

   Insert

   methyltransferase like 3 (METTL3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PB-3xCTCF-TetO

    Plasmid

    #213023

    Purpose

    piggyBac donor vector containing an 8-kb TetO operator array flanked by 3xCTCF sites for random integration using PBase transposase

    Depositor

    Luca Giorgetti

    Article

    Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

    Insert

    3xCTCF-TetO
    

    Use

    Mouse Targeting; Piggybase integration (transposo…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3XFlag pCMV5-TOPO TAZ WT

    Plasmid

    #24809

    Depositor

    Jeff Wrana

    Article

    Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

    Insert

    TAZ (WWTR1 Human)
    

    Use

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pJT121_GalL_nCDA1-VQRBE3

    Plasmid

    #145086

    Purpose

    Expressing base editor nCDA1-VQRBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    nCDA1-VQRBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A, D1135V, R1335Q, T1337R)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti Lifeact-iRFP670 BlastR

    Plasmid

    #84385

    Purpose

    Lentiviral expression of iRFP670-tagged Lifeact with Blasticidin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mutation

    Promoter

    CMV immediate early

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO-Cre stuffer v4

    Plasmid

    #158032

    Purpose

    lenti-viral construct with Cre recombinase and U6 driven sgRNA casette with modified tracrRNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pcDNA3.1(+)-HA-Flag-natT1R2

    Plasmid

    #113944

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    residues 22-839

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. 6599-SUMO1GG

    Plasmid

    #13377

    Depositor

    Anne Dejean

    Article

    Seeler et al Mol Cell Biol. 2001 May . 21(10):3314-24

    Insert

    SUMO1 (SUMO1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mature form ending with GG

    Promoter

    Available since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTS2362_Tier3(SB)-rtTA-PuroR

    Plasmid

    #169658

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven rtTA expression and PRPBSA-driven PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXs-IP spGFP-ERGIC53

    Plasmid

    #38270

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Autophagy. 2010 Aug;6(6):764-76.

    Insert

    ERGIC-53 (LMAN1 Human)
    

    Use

    Retroviral

    Tags

    EGFP with Calreticulin signal peptide at N-termin…

    Expression

    Mammalian

    Mutation

    deleted signal peptide (1-30 amino acids)

    Promoter

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG PSAM4 GlyR IRES EGFP

    Plasmid

    #119739

    Purpose

    Chemogenetic inhibitor expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    CAG

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-PE1

    Plasmid

    #132774

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    PE1
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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