We narrowed to 3,063 results for: PHI-1

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pX330-PITCh-TUBA1B

Plasmid

#207763

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of TUBA1B (TUBA1B Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Solo-mStayGold-TJP1

Plasmid

#227300

Purpose

Donor template for mStayGold insertion into the N-terminus of the TJP1 locus. For tight junction visualization. To be co-transfected with sgRNA plasmid px330-TJP1 (Addgene #227299)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

TJP1 Homology Arms flanking a mStayGold Tag (TJP1 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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EcNGsC-MutY-pKK223

Plasmid

#131376

Purpose

Low level expression of E.coli G.stearothermophillus MutY chimera protein

Depositor

Martin Horvath

Article

Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

Insert

EcNGsC MutY chimera

Expression

Bacterial

Mutation

Fusion of two MutY genes. Residues 1-225 are from…

Promoter

tac

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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PL-452 C-EGFP

Plasmid

#19178

Depositor

Patrik Verstreken

Article

Venken et al Nucleic Acids Res. 2008 Oct;36(18):e114.

Insert

EGFP

Use

Cre/Lox

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
PL-452 N-EGFP

Plasmid

#19173

Depositor

Patrik Verstreken

Article

Venken et al Nucleic Acids Res. 2008 Oct;36(18):e114.

Insert

EGFP

Use

Cre/Lox

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
PL-452 C-Flag-4C

Plasmid

#19180

Depositor

Patrik Verstreken

Article

Venken et al Nucleic Acids Res. 2008 Oct;36(18):e114.

Insert

FLAG tetracysteine tag

Use

Cre/Lox

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
PL-452 C-mDsRed

Plasmid

#19179

Depositor

Patrik Verstreken

Article

Venken et al Nucleic Acids Res. 2008 Oct;36(18):e114.

Insert

mDsRed

Use

Cre/Lox

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
PL-452 N-Flag-4C

Plasmid

#19175

Depositor

Patrik Verstreken

Article

Venken et al Nucleic Acids Res. 2008 Oct;36(18):e114.

Insert

FLAG tetracysteine tag

Use

Cre/Lox

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
PL-452 C-YPet

Plasmid

#19177

Depositor

Patrik Verstreken

Article

Venken et al Nucleic Acids Res. 2008 Oct;36(18):e114.

Insert

YPet

Use

Cre/Lox

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
PL-452 N-YPet

Plasmid

#19172

Depositor

Patrik Verstreken

Article

Venken et al Nucleic Acids Res. 2008 Oct;36(18):e114.

Insert

YPet

Use

Cre/Lox

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
PL-452 N-mDsRed

Plasmid

#19174

Depositor

Patrik Verstreken

Article

Venken et al Nucleic Acids Res. 2008 Oct;36(18):e114.

Insert

mDsRed

Use

Cre/Lox

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
PL-452 C-CyPet

Plasmid

#19176

Depositor

Patrik Verstreken

Article

Venken et al Nucleic Acids Res. 2008 Oct;36(18):e114.

Insert

CyPet

Use

Cre/Lox

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
PL-452 N-CyPet

Plasmid

#19171

Depositor

Patrik Verstreken

Article

Venken et al Nucleic Acids Res. 2008 Oct;36(18):e114.

Insert

CyPet

Use

Cre/Lox

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
EF2451_pET21a(+)

Plasmid

#13840

Depositor

SeWon Suh

Article

Kang et al Acta Crystallograph Sect F Struct Biol C

Insert

PPAT

Tags

His

Expression

Bacterial

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

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ClhN-HIS5

Plasmid

#207014

Purpose

Plasmid backbone for yeast expression, use auxotrophic marker HIS5 (Schizosaccharomyces Pombe)

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-MET15

Plasmid

#207013

Purpose

Plasmid backbone for yeast expression, use auxotrophic marker MET15 (Saccharomyces cerevisiae)

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-URA3

Plasmid

#207012

Purpose

Plasmid backbone for yeast expression, use auxotrophic marker URA3 (Kluyveromyces lactis)

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMT2 Beta1b GFP

Plasmid

#89893

Purpose

Mammalian expression of Beta1b GFP

Depositor

Annette Dolphin

Article

Page et al J Biol Chem. 2016 Sep 23;291(39):20402-1

Insert

Cacnb1 (Cacnb1 Rat)

Tags

GFP

Expression

Mammalian

Mutation

GFP sequence inserted just before the Stop codon

Promoter

adenovirus major late promoter

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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Cav2.1 HA pCAGGS

Plasmid

#206076

Purpose

expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Promoter

CMV/B-actin

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMT2 Beta1b mCherry

Plasmid

#89892

Purpose

Mammalian expression of Beta1b mCherry

Depositor

Annette Dolphin

Article

Page et al J Biol Chem. 2016 Sep 23;291(39):20402-1

Insert

Cacnb1 (Cacnb1 Rat)

Tags

mCherry

Expression

Mammalian

Mutation

mCherry sequence inserted just before the Stop co…

Promoter

adenovirus major late promoter

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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